Penggunaan Metode In Silico pada Formulasi Insektisida Alami Berbasis Minyak Serai Wangi dan Minyak Cengkeh serta Uji Aktivitas terhadap Serangga Ulat Grayak (Spodoptera litura)

Nindyasiwi, Putri (2017) Penggunaan Metode In Silico pada Formulasi Insektisida Alami Berbasis Minyak Serai Wangi dan Minyak Cengkeh serta Uji Aktivitas terhadap Serangga Ulat Grayak (Spodoptera litura). Sarjana thesis, Universitas Brawijaya.

Abstract

Insektisida alami merupakan salah satu jenis pestisida yang berbahan dasar alami berasal dari tumbuhan sebagai alternatif pestisida sintetis untuk membunuh serangga. Dalam penelitian ini telah dilakukan uji aktivitas insektisida alami berbasis minyak serai wangi dan minyak cengkeh. Formulasi insektisida ditentukan dengan pendekatan in silico menggunakan perangkat lunak AutoDock. Pada proses docking digunakan reseptor ulat grayak dengan kode PDB 2DJC, sedangkan ligan yang digunakan adalah ligan geraniol, sitronelal, sitronelol dan eugenol. Nilai Ki hasil docking digunakan untuk penentukan nilai IC50. Dari nilai IC50 ini dipadukan dengan kadar komponen utama minyak serai wangi dan minyak cengkeh yang digunakan untuk menentukan formulasi insektisida. Uji aktivitas insektisida diaplikasikan terhadap hama serangga ulat grayak (S.litura) dengan metode kontak racun dan kontak daun. Pengujian ini dilakukan selama 24 jam. Dari hasil uji in silico diperoleh formula insektisida minyak serai wangi dan minyak cengkeh yaitu 4 : 1. Hasil uji aktivitas konsentrasi 0,25% pada metode kontak racun dan 1% pada metode kontak daun efektif digunakan sebagai insektisida. Nilai LC50 dan LC90 berdasarkan analisis probit menggunakan SPSS untuk metode kontak racun adalah 0,052% dan 0,194%, sedangkan LC50 dan LC90 untuk metode kontak daun adalah 0,308% dan 0,476%.

English Abstract

Natural insecticide is one of nature-based pesticide from plants as an alternative of synthetic pesticide to kill insect. In this research has been tested the activity of natural insecticide based on citronella oil and clove oil. Insecticidal formulations were determined using the in silico approach using AutoDock software. Docking process used receptor Spodoptera litura with PDB code 2DJC, while ligands used are geraniol ligands, citronellal, citronellol and eugenol. Ki value of docking result is used for determination IC50 value. The value of IC50 is combined with the content of the main components of the lemon grass oil and clove oil used to determine insecticide formulations. Insecticidal activity test was applied to Spodoptera litura insect by contact method of toxic and leaf contact. This test is done for 24 hours. From the results of in silico test, the insecticide formula of citronella oil and clove oil is 4: 1. The result of 0.25% concentration activity test on toxic contact method and 1% on leaf contact method is effective as insecticide. The values of LC50 and LC90 based on probit analysis using SPSS for toxic contact method were 0.052% and 0.194%, whereas LC50 and LC90 for leaf contact method were 0.308% and 0.476%.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FMIPA/2017/344/051709684
Uncontrolled Keywords: pemodelan molekuler, minyak serai wangi, minyak cengkeh, aktivitas insektisida, LC50
Subjects: 600 Technology (Applied sciences) > 633 Field and plantation crops > 633.8 Other crops grown for industrial processing > 633.898 Insecticide-producing plants
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Nur Cholis
Date Deposited: 18 Oct 2017 06:48
Last Modified: 23 Nov 2021 06:56
URI: http://repository.ub.ac.id/id/eprint/3952
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