Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Kadar Malondialdehida Serum Darah dan Ekspresi HSP70 pada Otak Tikus (Rattus norvegicus) Model Traumatic Brain Injury

Kayeme, Asdia Nosase (2017) Pengaruh Terapi Ekstrak Kulit Manggis (Garcinia mangostana L.) terhadap Kadar Malondialdehida Serum Darah dan Ekspresi HSP70 pada Otak Tikus (Rattus norvegicus) Model Traumatic Brain Injury. Sarjana thesis, Universitas Brawijaya.

Abstract

Traumatic Brain Injury merupakan cedera yang terjadi karena adanya tekanan mekanik eksternal yang mengenai cranium dan komponen intrakranial, sehingga menimbulkan kerusakan sementara atau permanen pada otak. Pasca terjadinya cedera otak, terjadi over produksi radikal bebas yang menyebabkan stres oksidatif. Ekstrak kulit manggis mengandung xanthone yang bekerja sebagai antiinflamasi dan penangkal radikal bebas. Tujuan penelitian ini untuk mengetahui pengaruh terapi ekstrak kulit manggis terhadap penurunan kadar MDA serum darah dan ekspresi HSP70 pada otak tikus (Rattus norvegicus) model TBI. Penelitian ini menggunakan hewan coba tikus putih yang dikelompokkan menjadi 4 perlakuan yaitu kelompok negatif, kelompok positif (model TBI), kelompok TBI dengan terapi ekstrak kulit manggis dosis 0,5 mL/hari selama 5 hari dan kelompok TBI dengan terapi minocycline dosis 0,5 mL/hari selama 5 hari. Kadar MDA serum darah tikus diukur menggunakan uji Thiobarbituric Acid (TBA) sedangkan ekspresi HSP70 pada otak dianalisis secara deskriptif sel-sel yang terekspresi serta dihitung jumlah sel yang terekspresi. Hasil penelitian menunjukkan bahwa terapi ekstrak kulit manggis menurunkan kadar MDA serum darah sebesar 23,41%. Terapi ekstrak kulit manggis memperbaiki gambaran histopatologi dan menurunkan ekspresi HSP70 pada jaringan otak tikus sebesar 39,36%. Hasil analisa statistik uji kadar MDA dan ekspresi HSP70 menunjukkan bahwa terdapat perbedaan yang signifikan antar kelompok perlakuan (p<0,01). Penelitian ini dapat disimpulkan bahwa terapi ekstrak kulit manggis dapat digunakan untuk terapi pasca terjadinya cedera otak.

English Abstract

Traumatic Brain Injury is an injury that occurs due to external mechanical forces that affect the cranium and intracranial components, causing temporary or permanent damage to the brain. After the occurrence of brain injury, there is over-production of free radicals that cause oxidative stress. Mangosteen pericarp extract contains xanthone that work as anti-inflammatory and free radical antidote. The purpose of this study was to determine the effect of mangosteen pericarp extract therapy on decreasing blood serum MDA level and HSP70 expression of brain on TBI rats. This study used white mouse experimental animals grouped into 4 groups of treatment: negative group, positive group (TBI model), TBI group with mangosteen pericarp extract therapy dose 0.5 mL / day for 5 days and TBI group with minocycline therapy dose 0, 5 mL / day for 5 days. Blood serum MDA level was measured using Thiobarbituric Acid (TBA) test while HSP70 expression in brain was analyzed descriptively by expressed cells and calculated number of expressed cells. The results showed that mangosteen pericarp extract therapy decreased blood serum MDA level up to 23.41%. Treatment of mangosteen pericarp extracts improved histopathology and reduced HSP70 expression of brain up to 39.36%. The result of statistical analysis of MDA and HSP70 expression showed that there were significantly differences between treatment groups (p<0.01). This study can be concluded that mangosteen pericarp extract therapy can be used for therapy after the occurrence of brain injury.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FMIPA/2017/343/051709683
Uncontrolled Keywords: Garcinia mangostana L., HSP70, malondialdehida, traumatic brain injury.
Subjects: 500 Natural sciences and mathematics > 543 Analytical chemistry > 543.599 3 Analytical chemistry (Mammals-Mouse)
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam > Kimia
Depositing User: Nur Cholis
Date Deposited: 18 Oct 2017 06:43
Last Modified: 23 Nov 2021 06:57
URI: http://repository.ub.ac.id/id/eprint/3948
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