Salsabila, Nabila and dr. Holipah, PhD and dr. Dian Sukma Hanggara, Sp. PK, M.Biomed. (2024) Pengaruh Pemberian Kombinasi Metformin dan Lactobacillus plantarum Dad-13 terhadap Indeks Lee dan Profil Lipid Tikus Model NAFLD. Sarjana thesis, Universitas Brawijaya.
Abstract
Non-Alcoholic Fatty Liver Disease (NAFLD) adalah kasus penyakit kronis pada liver yang paling sering terjadi. Kasus NAFLD terus meningkat sejalan dengan peningkatan kasus obesitas dan sindrom metabolik akibat perubahan gaya hidup dan diet. Western Diet diketahui terlibat aktif dalam perkembangan obesitas, resistensi insulin dan disbiosis mikrobiota yang diperkirakan menjadi faktor yang mendasari patogenesis dan progresivitas NAFLD. Metformin diketahui menjadi obat yang cukup menjanjikan dalam meningkatkan sensitivitas insulin, menurunkan berat badan dan kadar lemak dalam darah. Lactobacillus plantarum Dad-13, probiotik asal Indonesia, diketahui dapat menyeimbangkan proporsi dan komposisi mikrobiota usus serta dapat menurunkan IMT pada pasien overweight. Kombinasi dua terapi farmakologi yang diberikan secara bersamaan diharapkan memberikan efek terapeutik yang sinergis dan lebih baik. Penelitian eksperimental ini bertujuan untuk menganalisis pengaruh pemberian kombinasi metformin dan L. plantarum Dad-13 terhadap Indeks Lee dan profil lipid tikus model NAFLD. Desain penelitian memakai posttest-only controlled group. Tikus Rattus norvegicus galur Wistar jantan sejumlah 21, dibagi kedalam 3 kelompok perlakuan : Kelompok Diet Normal (KDN), Kelompok Western Diet (KWD), Kelompok Metformin Probiotik (KMP). Diet diberikan selama 28 minggu setiap hari dan pemberian kombinasi metformin dan probiotik diberikan pada kelompok KMP di 10 minggu terakhir penelitian. Berdasarkan hasil analisis data, ditunjukkan bahwa secara signifikan terdapat perbedaan kadar trigliserida (p = 0,13) antara kelompok KDN dengan masing-masing kelompok lainnya yaitu KWD dan KMP. Pada variabel lain seperti Indeks Lee (p=0,226), HDL (p=0,159), LDL (p=0,389), dan Total Kolesterol (p=0,785) tidak ditemukan perbedaan yang signifikan.
English Abstract
Non-Alcoholic Fatty Liver Disease (NAFLD) is the most common chronic liver disease. Incidence of NAFLD continues to rise in parallel with the increasing cases of metabolic syndrome, including obesity, as a result of lifestyle and diet changes. Western Diet is acknowledged to actively contribute to several conditions such as obesity, insulin resistance, and gut microbiota dysbiosis, which are believed to be underlying factors in the disease's progression and the worsening of NAFLD. Metformin is known to be a promising drug in increasing insulin sensitivity, reducing body weight and blood fat levels. Lactobacillus plantarum Dad-13, a probiotic from Indonesia, is known to balance the proportion and composition of the intestinal microbiota and can reduce BMI in overweight patients. The combination of two pharmacological therapies given simultaneously is expected to provide a synergistic and better therapeutic effect. This experimental study aims to determine the effects of the combination of metformin and L. plantarum Dad-13 on the Lee Index and lipid profile of a NAFLD rat model. The research design uses a posttest-only controlled group. Male Wistar strain Rattus norvegicus rats (21 in total) were divided into three treatment groups: Normal Diet Group (KDN), Western Diet Group (KWD), and Metformin Probiotic Group (KMP). Diet was given every day for 28 weeks and a combination of metformin and probiotics was given to the KMP group in the last 10 weeks of the study. The results indicate a significant difference in triglyceride levels (p = 0.13) between the KDN group and each of the other groups, KWD and KMP. For other variables such as the Lee Index (p=0.226), HDL (p=0.159), LDL (p=0.389), and Total Cholesterol (p=0.785), no significant differences were found.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0524060072 |
| Uncontrolled Keywords: | Metformin, Lactobacillus plantarum Dad-13, Indeks Lee, profil lipid. |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Endang Susworini |
| Date Deposited: | 13 Feb 2024 06:06 |
| Last Modified: | 13 Feb 2024 06:06 |
| URI: | http://repository.ub.ac.id/id/eprint/214667 |
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