Pengaruh Pemberian Ekstrak Phaseolus Vulgaris L. Terhadap Persentase Sel Punca Mesenkimal Darah Perifer Dan Derajat Artritis Pada Tikus Model Artritis Reumatoid

Dantara, Tri Wahyudi Iman and Dr. dr. Cesarius Singgih Wahono, SpPD., K-R and dr. Perdana Aditya Rahman, SpPD-KR (2023) Pengaruh Pemberian Ekstrak Phaseolus Vulgaris L. Terhadap Persentase Sel Punca Mesenkimal Darah Perifer Dan Derajat Artritis Pada Tikus Model Artritis Reumatoid. Magister thesis, Universitas Brawijaya.

Abstract

Pendahuluan: Pengobatan berbasis sel punca mesenkimal dinilai cukup menjanjikan pada artritis reumatoid, namun selain mahal, teknologi pengembangannya masih belum berkembang di Indonesia. Kacang hitam (Phaseolus vulgaris L.) merupakan bahan pakan yang murah dan mudah ditemui. Tujuan: Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak Phaseolus vulgaris L. terhadap persentase sel punca mesenkimal dan derajat artritis pada hewan coba model artritis reumatoid. Metode: Sebanyak 28 tikus Wistar betina umur 7-12 minggu dibagi menjadi 7 kelompok: tanpa perlakuan [K(-)], hewan coba model artritis reumatoid [K(+)], K(+) + Plerixafor 1,25 mg/kgBB/hari (KP1), K(+) + metotreksat 0,2 mg/kgBB/hari (KP2), dan K(+) + ekstrak Phaseolus vulgaris L. dosis [100 mg (P1); 500 mg (P2) dan 1.000 mg/kgBB/hari (P3)]. Pembuatan hewan coba dilakukan dengan memberikan imunisasi emulsi kolagen tipe II dan adjuvan Freund tidak lengkap sebanyak 2 dosis. 21 hari pasca imunisasi pertama, masing-masing perlakuan diberikan selama 21 hari. Derajat artritis dihitung menggunakan skor artritis. Persentase sel punca mesenkimal (CD29+CD90+CD45-) pada darah diukur menggunakan flowsitometri. Hasil Penelitian: Ekstrak Phaseolus vulgaris L. dosis 1.000 mg/kgBB menurunkan derajat artritis (8.00 ± 5.42 vs 13.25 ± 2.22; p=0.03) dan meningkatkan persentase sel punca mesenkimal (55.87 ± 10.83 vs 28.18 ± 9.65 %; p=0.04) darah perifer secara signifikan pada tikus model artritis reumatoid. Kesimpulan: Ekstrak Phaseolus vulgaris L. meningkatkan persentase sel punca mesenkimal darah perifer dan menurunkan derajat artritis pada tikus model artritis reumatoid.

English Abstract

Background: Mesenchymal stem cell-based treatment is considered quite prom-ising in rheumatoid arthritis, but apart from being expensive, its development technology is still underdeveloped in Indonesia. Black bean (Phaseolus vulgaris L.) is a cheap feed ingredient and easy to find. Aim: This study aims to examine the effect of various doses of Phaseolus vulgaris L. extract on the degree of arthritis and the percentage of mesenchymal stem cells in rheumatoid arthritis animal models. Methods: Amount of 28 female Wistar rats aged 7-12 weeks were divided into 7 groups: no treatment [K(-)], rheumatoid arthritis model experimental animals [K(+)], K(+) + Plerixafor 1.25 mg/kgBW/ intraperitoneal day (KP1), K(+) + methotrexate 0.2 mg/kgBW/day, orally (KP2), and K(+) + Phaseolus vulgaris L. extract orally various doses [100 mg (P1); 500 mg (P2) and 1,000 mg/kgBW/day (P3). Arthritis rheumatoid rat models were made by immunizing 2 doses of type II collagen emulsion and incomplete Freund's adjuvant. 21 days after the first immunization, each treatment was given for 21 days. The degree of arthritis is calculated using the arthritis score. The percentage of mesenchymal stem cells (CD29+CD90+CD45-) in blood was measured using flow cytometry. Results: Administration of Phaseolus vulgaris L. extract at a dose of 1,000 mg/kgBW increased the percentage of mesenchymal stem cells (55.87 ± 10.83 vs 28.18 ± 9.65 %; p=0.04) and reduced the degree of arthritis (8.00 ± 5.42 vs 13.25 ± 2.22; p=0.03) significantly in rheumatoid arthritis rat models. Conclusion: Phaseolus vulgaris L. extract increases the percentage of peripheral blood mesenchymal stem cells and reduces the degree of arthritis in rheumatoid arthritis rat models.

Other obstract

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Item Type: Thesis (Magister)
Identification Number: '0423060008
Uncontrolled Keywords: Artritis reumatoid, Phaseolus vulgaris L., sel punca mesenkimal, artritis, kacang hitam
Subjects: 600 Technology (Applied sciences) > 616 Diseases
Divisions: Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran
Depositing User: Endang Susworini
Date Deposited: 17 Jul 2023 09:02
Last Modified: 17 Jul 2023 09:02
URI: http://repository.ub.ac.id/id/eprint/201906
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