Jat, Wiwit Wicaksono and Prof. Dr. Abdul Latief Abadi, and Luqman Qurata Aini (2022) Seleksi Isolat Trichoderma sp. Sebagai Agen Hayati Penyakit Busuk Akar dan Pangkal Batang Tebu (Xylaria sp.). Magister thesis, Universitas Brawijaya.
Abstract
Penyakit pada tanaman tebu merupakan faktor pembatas produktivatas. Penyakit busuk akar dan pangkal batang tebu Xylaria sp. di Lampung, Sumatra Selatan menyebabkan kerugian hasil yang tinggi. Serangan penyakit 25% dan 26% dapat menurunkan produksi gula berturut-turut 12.3% dan 15.4%. Pengendalian yang dilakukan menggunakan fungisida di kebun tebu tidak efektif dan mahal. Oleh karena itu diperlukan pengendalian menggunakan agen hayati. Tujuan penelitian ini adalah Memperoleh Isolat jamur Trichoderma sp. Yang dapat digunakan sebagai agen hayati dalam mengendalikan penyakit busuk akar dan pangkal batang tebu Xylaria sp., Mengetahui mekanisme penghambatan patogen Xylaria sp., dan mengetahui spesies Trichoderma sp. sebagai agen hayati terhadap jamur Xylaria sp. Penelitian ini dilakukan di Laboratorium Penyakit dan Laboratorium analisa terpadu atau Laboratorium Jasa Pusat Penelitian Perkebunan Gula Indonesia serta Laboratorium Pusat Penelitian Bioteknologi dan Bioindustri Indonesia, Bogor, Jawa Barat. Metode yang dilakukan menggunakan metode seleksi bertahap. Seleksi awal dilakukan terhadap kerapatan dan viabilitas Trichoderma sp., seleksi kedua berdasarkan uji antagonisme dual kultur dan uji 2 sisi terhadap isolat terpilih pada seleksi awal. Tahap ketiga pada isolat terpilih dilakukan pengujian indeks kitinolitik dan penghitingan jumlah ekstrak kasar kitinase yang dinyatakan dengan aktivitas enzim kitinase. Pemilihan isolat calon agen hayati berdasarkan hasil uji antagonis dan nilai indeks kitinolotik tertinggi. Isolat terpilih dilakukan identifikasi secara molekuler dengan sequensing DNA. Hasil penelitian menunjukkan bahwa isolat T10 merupakan isolat yang dapat menghambat pertumbuhan Xylaria sp. Mekanisme antagonism yaitu kompetisi, antibiosis dan mikoparasitisme. Mekanisme mikoparasitisme ditandai dengan rusaknya hifa dan lilitan hifa jamur antagonis serta aktivitas enzim kitinase yang tinggi. Isolat Trichoderma sp. T10 teridentifikasi sebagai Trichoderma asperellum
English Abstract
Disease in sugarcane is a limiting factor for productivity. Disease root and base rot of sugarcane stems Xylaria sp. in Lampung, South Sumatra caused high yield losses. Disease attacks of 25% and 26% can reduce sugar production by 12.3% and 15.4%, respectively. Controls carried out using fungicides in sugarcane plantations are ineffective and expensive. Therefore it is necessary to control using biological agents. The purpose of this study was to obtain isolates of the fungus Trichoderma sp. Which can be used as biological agents in controlling root and stem rot disease of sugarcane Xylaria sp., knowing the mechanism of inhibition of Xylaria sp. pathogens, and knowing Trichoderma sp. species. as a biological agent against the fungus Xylaria sp. This research was conducted at the Disease Laboratory and the Integrated Analysis Laboratory or the Services Laboratory of the Indonesian Sugar Plantation Research Center and the Indonesian Center for Biotechnology and Bioindustry Research Laboratory, Bogor, West Java. The method used is the stepwise selection method. The initial selection was carried out on the density and viability of Trichoderma sp., second selection based on dual culture antagonism test and 2-sided test on isolates selected in the initial selection. In the third step, the selected isolates were tested for the chitinolytic index and calculated the amount of the crude extract of chitinase which was expressed by the activity of the chitinase enzyme. Selection of candidate isolates of biological agents based on the results of the antagonist test and the highest chitinolytic index value. Selected isolates were identified molecularly by DNA sequencing. The results showed that the T10 isolate was an isolate that could inhibit the growth of Xylaria sp. The mechanisms of antagonism are competition, antibiosis and mycoparasitism. The mechanism of mycoparasitism is characterized by the destruction of hyphae and hyphae binding of antagonistic fungi and high chitinase enzyme activity. Isolates of Trichoderma sp. T10 was identified as Trichoderma asperellum
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | 0422040045 |
| Uncontrolled Keywords: | antagonisme, kitinolotik, tanaman tebu, Trichoderma sp., Xylaria sp, antagonism, chitinolytic, sugarcane, Trichoderma sp., Xylaria sp. |
| Subjects: | 600 Technology (Applied sciences) > 630 Agriculture and related technologies |
| Divisions: | S2/S3 > Magister Ilmu Tanaman, Fakultas Pertanian |
| Depositing User: | Nur Cholis |
| Date Deposited: | 16 Jan 2023 04:21 |
| Last Modified: | 16 Jan 2023 04:21 |
| URI: | http://repository.ub.ac.id/id/eprint/196644 |
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