Sekarsari, Prajana Luci and Luqman Qurata Aini,, S.P., M.Si., Ph.D (2020) Eksplorasi Bakteri Rizosfer Tanaman Wortel Organik Yang Berpotensi Sebagai Agens Pengendali Hayati Pectobacterium Carotovorum Penyebab Penyakit Busuk Lunak Pada Tanaman Wortel. Sarjana thesis, Universitas Brawijaya.
Abstract
Wortel adalah salah satu jenis sayuran yang banyak dikonsumsi oleh penduduk Indonesia. Produksi wortel seringkali mengalami penurunan akibat organisme pengganggu tanaman. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum adalah salah satu penyakit terpenting pada wortel yang menyebabkan penurunan produksi wortel. Para petani wortel lebih suka menggunakan pestisida kimiawi untuk mengendalikan P. carotovorum. Namun, berdampak negatif terhadap lingkungan. Penggunaan pengendalian biologis dengan organisme biokontrol merupakan metode yang ramah lingkungan. Organisme biokontrol yang dapat digunakan adalah bakteri antagonis dari rizosfer tanaman wortel organik. Tujuan dari penelitian ini adalah untuk mengetahui beberapa bakteri antagonis dari rhizosfer wortel organik yang dapat mengendalikan P. carotovorum. Penelitian ini dilaksanakan pada bulan Januari-Juli 2020 di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Kota Malang. Tahapan penelitian yaitu pengambilan sampel tanah dari rizosfer tanaman wortel organik, eksplorasi bakteri rizosfer dari rizosfer tanaman wortel organik, isolasi bakteri patogen P. carotovorum, karakterisasi dan identifikasi bakteri patogen P. carotovorum, uji patogenisitas terhadap isolat patogen pada tanaman wortel, seleksi uji antagonis bakteri rizosfer terhadap patogen P. carotovorum dan uji hipersensitif, uji antagonis bakteri rizosfer terhadap bakteri P. carotovorum, uji in vivo penekanan penyakit busuk lunak pada umbi wortel dengan bakteri antagonis terpilih, karakterisasi morfologi bakteri antagonis. Berdasarkan hasil penelitian yang telah dilakukan, diperoleh 76 bakteri hasil eksplorasi rizosfer tanaman wortel organik. Terdapat 5 isolat bakteri (K7, K10, K11, M4, M19) yang telah terpilih memiliki zona hambat terbesar yang digunakan dalam uji antagonis terhadap patogen P. carotovorum. Pada pengujian secara in vitro, perlakuan bakterisida menunjukkan rerata zona hambat terbesar dibandingkan perlakuan lainnya. Sedangkan pada pengujian secara in vivo menunjukkan hasil bahwa seluruh perlakuan bakteri rizosfer antagonis dan perlakuan dengan Streptomycin sulfat memiliki kemampuan menekan gejala penyakit P. carotovorum yang setara. Kemudian, hasil identifikasi morfologi pada 5 isolat bakteri antagonis memiliki karakteristik berbeda-beda dengan sebagian besar koloni bakteri yang didapatkan berwarna putih, berbentuk bulat, berukuran kecil, berelevasi cembung, memiliki tepi koloni rata dengan permukaan koloni halus
English Abstract
Carrots are one type of vegetable that is widely consumed by Indonesian residents. The carrot production often has decreased due to plant disturbing organism. Soft rot disease caused by Pectobacterium carotovorum is one of the most important diseases on carrot causing the decrease of carrot production. The carrot farmers prefer to use chemical pesticides to control P. carotovorum. But, it had a negative impact on the environment. The use of biologically control with biocontrol organisms is an eco-friendly method. Biocontrol organisms that can be used are antagonistic bacteria from the rhizosphere of organic carrot plants. The purpose of this study was to determine some antagonist bacteria from the rhizosphere of the organic carrot that can control P. carotovorum. This research was conducted in January-July 2020 at the Plant Disease Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University, Malang City. Stages of research are soil sampling from the rhizosphere of organic carrot plants, exploration of rhizosphere bacteria from the rhizosphere of organic carrot plants, isolation of pathogenic bacteria P. carotovorum, characterization and identification of pathogenic bacteria P. carotovorum, pathogenicity test of pathogenic isolates in carrot plants, selection of bacterial antagonistic tests rhizosphere to pathogen P. carotovorum and hypersensitivity test, antagonist test of rhizosphere bacteria to P. carotovorum bacteria, in vivo test suppression of soft rot in carrot bulbs with selected antagonistic bacteria, morphological characterization of antagonistic bacteria. Based on the results of research that has been done, obtained 76 bacteria rhizosphere exploration result of organic carrots. There are 5 bacterial isolates (K7, K10, K11, M4, M19) with the largest inhibition zone used in the antagonists test against the pathogens P. carotovorum. From in vitro testing, bactericide treatment showed the largest average inhibition zone compared to other treatments. Meanwhile, the in vivo test showed that all treatments of rhizosphere antagonist bacteria and treatment with Streptomycin sulfate had the same ability to suppress the symptoms of P. carotovorum. Then, the morphological identification results of the five antagonistic bacterial isolates had different characteristics with most of the bacterial colonies that were found to be white, round, small in size, convex elevated, had flat colony edges with smooth colony surfaces.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520040063 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.6 Animal pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 10 Oct 2022 01:25 |
| Last Modified: | 10 Oct 2022 01:25 |
| URI: | http://repository.ub.ac.id/id/eprint/195445 |
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