Efek Paparan Partikel Debu Batu Bara Terhadap Aktivitas SOD dan Kerusakan Oksidatif DNA pada Mencit Model Asma.

Budianto, Windy Yuliana (2018) Efek Paparan Partikel Debu Batu Bara Terhadap Aktivitas SOD dan Kerusakan Oksidatif DNA pada Mencit Model Asma. Magister thesis, Universitas Brawijaya.

Abstract

Asma merupakan inflamasi kronis saluran pernapasan yang dapat diperparah dengan paparan alergen secara terus menerus. Partikel debu batu bara merupakan jenis alergen yang didapat dari polutan udara yang diketahui dapat meningkatkan hipersensitivitas, inflamasi saluran pernapasan, dan menimbulkan stres oksidatif pada kondisi asma. Meski demikian, hingga saat ini patomekanisme asma dan debu batu bara belum diketahui secara pasti. Oleh karena itu, penelitian ini bertujuan untuk mengetahui efek paparan partikel debu batu bara terhadap aktivitas SOD dan kerusakan oksidatif DNA pada mencit model asma. Sebanyak 24 ekor mencit balb/c betina dibagi kedalam empat kelompok. Kelompok pertama merupakan kelompok kontrol. Kelompok kedua adalah mencit yang diberi paparan inhlasi partikel debu batu bara PM5 12,5 mg/m3 selama 4 minggu. Kelompok ketiga yaitu mencit yang disensitisasi OVA melalui injeksi intraperitoneal dan inhalasi OVA 1% dalam NaCl 8 mL 3 kali dalam seminggu selama 8 minggu. Kelompok keempat adalah mencit yang disensitisasi OVA melalui injeksi intraperitoneal dan inhalasi OVA 1% dalam NaCl 8 mL 3 kali dalam seminggu selama 8 minggu serta diberi paparan inhalasi partikel debu batu bara PM5 12,5 mg/m3 selama 4 minggu. Pengolahan partikel debu batu bara dilakukan menggunakan alat pulverizer yang terdiri dari Ball, Ring, dan Raymond Mild di Laboratorium Carsurin Coal Banjarmasin. Proses tersebut menghasilkan partikel debu batu bara berukuran 75 μm. Agar partikel debu batubara dapat masuk ke dalam saluran pernapasan bawah, maka partikel debu batubara tersebut kemudian di lakukan penyaringan kembali menggunakan filter Mesh MicroSieve (BioDesign, Newyork, Ny, USA). sehingga diperoleh partikel debu batubara dengan diameter 5 μm (PM5). Sensitisasi OVA yang diberikan adalah albumin yang berasal dari Dried Egg White, crude (nomer produk A0198, TCI Chemical, India, Pvt, Ltd). Sensitisasi awal diberi melalui injeksi OVA secara intraperitoneal pada hari ke-0 dan 14. Sensitisasi ulang diberikan pada hari ke-21melalui inhalasi OVA 1% dengan menggunakan ultrasonic nebulizer Omron (tipe NE-U17, Shimadzu, Konotsubo, Terado-cho, Muko, Kyoto, Japan) selama 20 menit secara berkala 3 kali dalam seminggu selama 8 minggu. Stres oksidatif dilihat dengan mengukur aktivitas SOD dengan metode NBT, dan konsentrasi 8-OHdG dengan enzyme linked immunosorbent assay (ELISA) kit mouse 8-Hydroxy-desoxyguanosine (Cat.No. E0187Mo, Bioassay Technology Laboratory, Shanghai, China). Hasil penelitian menunjukkan tidak terdapat penurunan yang signifikan pada aktivitas SOD serum serta tidak terdapat peningkatan yang signifikan pada konsentrasi 8-OHdG serum dari masing-masing kelompok (p>0,05). Paparan partikel debu batu bara selama 4 minggu belum mampu menyebabkan penurunan aktivitas SOD serum dan peningkatan 8-OHdG serum pada mencit model asma

English Abstract

Asthma is a chronic inflammation of the respiratory tract which can be aggravated by continuous exposure to allergens. Coal dust particles are a type of allergen obtained from air pollutants that are known to increase hypersensitivity and inflammation of the respiratory tract as well as trigger the oxidative stress in asthma conditions. However, to date, coal dust and asthma pathomechanism remain unclear. Thus, this study aimed to examine the effect of coal dust particulatematter exposure on the SOD activity and the oxidative DNA damage in asthma mice model. Twenty-fourth female balb/c mice were divided into four groups. The first group was the controlled group. The second group was composed of mice exposed to 12.5 mg/m3 PM5 coal dust for four weeks. The third group was composed of OVA-sensitized mice by peritoneal injection and inhalation of 1% OVA diluted in 8 mL NaCl, three times a week for eight weeks. The fourth group was composed of OVA-sensitized mice by peritoneal injection and inhalation of 1% OVA diluted in 8 mL NaCl, three times a week for eight weeks, and exposed to inhalation of 12.5 mg/m3 PM5 coal dust particles for four weeks. The coal dust particles were produced using pulverizer consisting of Ball, Ring, and Raymond Mill at Carsurin Coal Laboratory in Banjarmasin. The process produced coal dust particles with a diameter of 75 μm. Coal dust particle that was engineered to reach the lower respiratory tract was confirmed to have the diameter of fewer than 5 μm (PM5). It was obtained by filtering the coal dust that has been grinded priorly using a Mesh MicroSieve (BioDesign, Newyork, Ny, USA). The OVA used was albumin from Dried Egg White, crude (Product number A0198, TCI Chemical, India, Pvt. Ltd). The initial sensitization was performed by intraperitoneally injecting of OVA. The re-sensitization was performed on day 21 by inhalation of 1% OVA using Omron Ultrasonic Nebulizer (type NE-U17, Konotsubo, Terado-cho, Muko, Kyoto, Japan) for 20 minutes periodically three times a week for eight weeks. The oxidative stress was examined by measuring the SOD activity employing the NBT method, and the 8-OHdG concentration using the mouse 8- Hydroxy-deoxyguanosine enzyme linked immunosorbent assay (ELISA) kit (Cat. No.E0187Mo, Bioassay Technology Laboratory, Shanghai, China). The results reported that there were no significant decrease in SOD activity and also no significant increase in 8-OHdG serum concentration from each group (p>0.05). Combination of OVA and coal dust showed the worst effect on IL-13, 8-OHdG, and SOD activity as well. Coal dust exposure for four weeks isn’t adequate to induce oxidative DNA damage systemically in asthma mice model.

Other obstract

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Item Type:	Thesis (Magister)
Identification Number:	TES/614.581/BUD/e/2018/041901093
Subjects:	600 Technology (Applied sciences) > 614 Forensic medicine; incidence of injuries, wounds, disease; public preventive medicine > 614.5 Incidence of and public measure to prevent specific disease and kinds of diseases
Divisions:	S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran
Depositing User:	Endang Susworini
Date Deposited:	30 Sep 2022 01:32
Last Modified:	30 Sep 2022 01:32
URI:	http://repository.ub.ac.id/id/eprint/195187

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