Potensi Metabolit Sekunder Bakteri Rizosfer Ub Forest Dalam Menghambat Penyakit Hawar Daun Bakteri (Xanthomonas Oryzae Pv.Oryzae) Pada Tanaman Padi

A’yun, Elly Qurrotu and Prof. Dr. Ir. Abdul Latief Abadi,, MS. and Restu Rizkyta Kusuma,, SP., MP., M.Sc (2021) Potensi Metabolit Sekunder Bakteri Rizosfer Ub Forest Dalam Menghambat Penyakit Hawar Daun Bakteri (Xanthomonas Oryzae Pv.Oryzae) Pada Tanaman Padi. Sarjana thesis, Universitas Brawijaya.

Abstract

vii RINGKASAN ELLY QURROTU A’YUN 165040207111111. Potensi Metabolit Sekunder Bakteri Rizosfer UB Forest dalam Menghambat Penyakit Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) Pada Tanaman Padi. Di bawah bimbingan Prof. Dr. Ir. Abdul Latief Abadi, MS. dan Restu Rizkyta Kusuma, SP., MP., M.Sc Padi menjadi sumber kebutuhan pangan manusia hampir setengah dari dunia termasuk Indonesia. Salah satu permasalahan tanaman padi diantaranya yaitu serangan penyakit Hawar Daun Bakteri (HDB) yang disebabkan oleh bakteri Xanthomonas oryzae pv. oryzae (Xoo). Penyakit HDB menyerang pada stadia anakan, generatif dan pemasakan, dapat juga menyerang pada malai tanaman padi, yang menyebabkan pengisian gabah padi tidak maksimal. Dampak dari serangan penyakit HDB menyebabkan penurunan hasil panen yang bervariasi, tergantung stadia tanaman terserang. Persentase kerugian berupa penurunan hasil akibat dari serangan HDB bisa mencapai 50%. Tujuan penelitian ini mengetahui potensi bakteri rizosfer UB Forest dan metabolit sekundernya sebagai agen antagonis terhadap hawar daun bakteri dan meningkatkan ketahanan tanaman padi terhadap Xoo. Penelitian dilaksanakan mulai bulan Desember 2019 sampai Agustus 2020 di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya, Malang. Pelaksanaan penelitian ini dilakukan beberapa tahapan yaitu: pembuatan media NA untuk menumbuhkan bakteri Xoo dan isolat bakteri rizosfer UB Forest, purifikasi bakteri Xoo dan rizosfer UB Forest, uji patogenesitas, uji antagonis bakteri Xoo dengan bakteri rizosfer UB Forest, ekstraksi metabolit sekunder bakteri rizosfer UB Forest yang memiliki sifat antagonis, uji metabolit sekunder bakteri rizosfer UB Forest secara in vitro, pembibitan padi di green house dan pengaplikasian hasil ekstraksi metabolit sekunder secara in vivo, dan analisis data. Hasil penelitian menunjukkan bahwa hasil dari uji patogenesitas gejala hawar daun bakteri muncul setalah 3 hari inokulasi pada daun padi dengan cara menggunting daun kemudian direndam pada suspensi Xoo. Hasil uji antagonis dari 15 bakteri rizosfer UB Forest terdapat 11 bakteri yang bersifat antagonis terhadap Xoo. Hasil uji antagonis dilakukan uji lanjut DMRT 5%, kemudian diambil 3 isolat bakteri dengan indeks penghambatan tertinggi sebesar 2.78 cm, 1.78 cm dan 1.45 cm yaitu Bacillus sp. N3, Pantoea sp. N23 dan Clostridium sp. N10. Hasil uji antibiosis menunjukkan 11 bakteri tersebut bersifat bakteriostatik. Metabolit sekunder isolat Bacillus sp. N3, Pantoea sp.N23 dan Clostridium sp. N10 juga menunjukkan kemampuan penghambatan terhadap pathogen Xoo sebesar 2.63 cm, 2.55 cm, dan 2.55 cm, meskipun masih lebih rendah dibandingkan kontrol. Pada percobaan di pembibitan padi, menunjukkan aplikasi bakteri rizosfer dan metabolit sekundernya berpengaruh terhadap persentase perkecambahan, panjang akar dan persentase serangan hawar daun. Persentase perkecambahan tertinggi yaitu pada perlakuan Bacillus sp. N3 dan Pantoea sp. N10 sedangkan persentase terendah pada perlakuan bakterisida. Panjang akar tertinggi pada perlakuan Bacillus sp. N3. Persentase serangan hawar daun terendah pada Bacillus sp. N3 , Pantoea sp. N23 dan matabolit sekunder Bacillus sp. yang dibandingkan dengan kontrol postif dan bakterisida

English Abstract

Rice is a source of human food needs for almost half of the world, including Indonesia. One of the problems in rice plants is the attack of Bacterial Leaf Blight (HDB) which is caused by the bacteria Xanthomonas oryzae pv. oryzae (Xoo). HDB disease attacks the tiller, generative and ripening stage, it can also attack rice panicles, which causes the filling of paddy grain is not optimal. The impact of HDB disease causes a decrease in yields that vary, depending on the stage of the plant affected. The percentage of losses in the form of reduced yield due to HDB attacks can be up to 50%. The purpose of this study was to determine the potential of UB Forest rhizosphere bacteria and their secondary metabolites as antagonistic agents against bacterial leaf blight and the potential in increasing the resistance of rice plants to Xoo. This research was conducted from December 2019 to August 2020 at the Plant Disease Laboratory, Agriculture Faculty, Brawijaya University, Malang. The implementation of this research was carried out in several stages, namely: making NA media to grow Xoo bacteria and UB Forest rhizosphere bacteria isolates, purification of Xoo bacteria and UB Forest rhizosphere, pathogenicity test, Xoo bacteria antagonism test with UB Forest rhizosphere bacteria, extraction of secondary metabolites of UB Forest rhizosphere bacteria which have antagonistic properties, in vitro test for secondary metabolites of UB Forest rhizosphere bacteria, rice nurseries in green houses and application of secondary metabolite extraction results in vivo, and data analysis. The results showed that the results of the pathogenicity test of bacterial leaf blight symptoms appeared after 3 days of inoculation on rice leaves. The antagonist test results of 15 UB Forest rhizosphere bacteria contained 11 bacteria that were antagonistic to Xoo. The results of the antagonist test were carried out further DMRT 5% test, then 3 bacterial isolates were taken with the highest inhibition indexes of 2.78 cm, 1.78 cm and 1.45 cm, namely Bacillus sp. N3, Pantoea sp. N23 and Clostridium sp. N10. The results of the antibiosis test showed that the 11 bacteria were bactericidal. Secondary metabolites of Bacillus sp. N3, Pantoea sp. N23 and Clostridium sp. N10 also showed an inhibitory ability against pathogen Xoo of 2.63 cm, 2.55 cm, and 2.55 cm, although they were still lower than the control. Experiments in rice nurseries showed that the application of rhizosphere bacteria and their secondary metabolites affected the germination percentage, root length and reduced the percentage of leaf blight attack. The highest germination percentage was in the treatment of Bacillus sp. N3 and Pantoea sp. N10 while the lowest percentage was in the bactericide treatment of 88%. The highest root length in the treatment of Bacillus sp. N3. The lowest percentage of leaf blight attack on Bacillus sp. N3, Pantoea sp. N23 and the secondary metabolites Bacillus sp. which were compared with positive control treatment and bactericid

Other obstract

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Item Type: Thesis (Sarjana)
Identification Number: 0520040006
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.6 Animal pests
Divisions: Fakultas Pertanian > Hama dan Penyakit Tanaman
Depositing User: Nur Cholis
Date Deposited: 27 Sep 2022 04:12
Last Modified: 27 Sep 2022 04:12
URI: http://repository.ub.ac.id/id/eprint/194951
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