Pengaruh Suplementasi Vitamin E Terhadap Kadar Malondialdehid (Mda) Plasma Pada Individu Dengan Aktivitas Fisik Submaksimal

Permadi, Gerry and dr. Putu Moda Arsana,, SpPD-KEMD and dr. Laksmi Sasiarini,, SpPD-KEMD (2018) Pengaruh Suplementasi Vitamin E Terhadap Kadar Malondialdehid (Mda) Plasma Pada Individu Dengan Aktivitas Fisik Submaksimal. Magister thesis, Universitas Brawijaya.

Abstract

Peserta Program Pendidikan Dokter Spesialis I Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Brawijaya, Rumah Sakit Umum Daerah dr. Saiful Anwar Malang 2.3Supervisor, Konsultan Divisi Endokrinologi, Metabolik dan Diabetes, Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Brawijaya Malang, Rumah Sakit Umum Daerah dr.Saiful Anwar Malang Latar belakang: Vitamin E adalah salah satu zat antioksidan yang cukup poten dan menjadi pilihan suplemen antioksidan oleh masyarakat karena harganya relatif murah dan banyak tersedia di pasaran. Aktivitas fisik submaksimal merupakan salah satu penyebab peningkatan radikal bebas. Peningkatan radikal bebas dapat dideteksi dengan peningkatan MDA yaitu produk akhir dari peroksidasi lemak. Tujuan Penelitian: Mengetahui efek suplementasi antioksidan vitamin E terhadap radikal bebas yang ditimbulkan oleh aktivitas fisik submaksimal dengan mengukur MDA plasma. Metode penelitian: Double blind controlled trial pada individu dengan aktivitas fisik submaksimal yaitu lari 1500 m dalam 12 menit menggunakan treadmill dengan kecepatan 7,5 km/jam. Subjek berjumlah 20 orang mahasiswa Fakultas Ilmu Olahraga Universitas Negeri Malang, terbagi atas 2 kelompok, yaitu kelompok perlakuan diberikan vitamin E (α-tokoferol 100 IU) dan kelompok kontrol diberikan plasebo. Suplemen diberikan satu tablet per hari selama empat belas hari sebelum aktivitas fisik submaksimal. Kadar MDA plasma diambil sebelum, 2 jam dan 48 jam setelah aktivitas fisik submaksimal. Analisis hasil penelitian menggunakan uji ANOVA dan post hoc dengan Tukey. Penelitian telah dinilai layak secara etik oleh tim Komisi Etik Penelitian Kesehatan Fakultas Kedokteran Universitas Brawijaya. Hasil: Terdapat peningkatan kadar MDA plasma pada 2 jam dan 48 jam setelah aktivitas fisik submaksimal pada kelompok kontrol dan kelompok dengan suplementasi vitamin E. Kadar MDA plasma pada kelompok dengan suplementasi vitamin E lebih rendah dan berbeda secara signifikan (p<0.05) pada kondisi basal dan 2 jam setelah aktivitas fisik submaksimal dibandingkan kelompok kontrol. Kesimpulan: Aktivitas fisik submaksimal meningkatkan kadar MDA plasma. Vitamin E dapat mencegah kenaikan kadar MDA plasma pada kondisi basal dan 2 jam setelah aktivitas fisik submaksimal yang diduga melalui mekanisme sebagai scavengers radikal bebas dan meningkatkan produksi antioksidan endogen melalui peningkatan ekspresi Nuclear factor erythroid 2-related factor 2 (Nrf2)

English Abstract

1Resident at Internal Medicine Department, Medical Faculty Brawijaya University, Saiful Anwar General Hospital, Malang 2,3Supervisor and Consultant at Division of Endocrinology, Metabolic Disease, and Diabetes Mellitus, Internal Medicine Department, Medical Faculty Brawijaya University, Saiful Anwar General Hospital, Malang Background: Vitamin E is among the potent anti-oxidant which become popular choice in society. It is relatively cheap and widely available in the market. Submaximal physical activity can cause increase of free radicals in circulation. Increase of free radicals can be detected through the increase of MDA which is the end product of lipid peroxidation. Objectives: This study aimed to measure the effect of Vitamin E supplementation against the free radicals from submaximal physical activity with MDA plasma concentration measurement. Method: A double blind controlled trial was performed in subjects with submaximal physical activity, which was defined as 1500 meters running in 12 minutes using treadmill with the speed of 7.5 km/hour. This study included 20 subjects from Sport Science Faculty of Malang University. The subjects were then divided into 2 groups. The treatment group was given vitamin E supplementation (α-tocopherol 100 IU) and control group was given placebo. Vitamin E supplementation was given 1 tablet a day for 14 days before the subjects were exposed to submaximal physical activity. Plasma MDA concentrations were measured before, 2 hours after, and 48 hours after the submaximal physical activity. Analysis of data was performed using ANOVA test with post hoc Tukey test. This study has been accepted by Ethical Committee of Medical Research, Medical Faculty, Brawijaya University. Results: There was an increase in plasma MDA levels at 2 hours and 48 hours after submaximal physical activity both in control group and group with vitamin E supplementation. Plasma MDA levels in the group with vitamin E supplementation were lower and significantly different (p <0.05) in basal conditions and 2 hours after submaximal physical activity compared to the control group. Conclusion: Submaximal physical activity increases plasma MDA levels. Vitamin E can prevent the increase in plasma MDA levels in basal conditions and 2 hours after submaximal physical activity which is suspected by the mechanism as free radical scavengers and increase the production of endogenous antioxidants through increased expression of Nuclear factor erythroid 2-related factor 2 (Nrf2

Other obstract

Item Type:	Thesis (Magister)
Identification Number:	TES/615.328/FK/p/2018/041810963
Uncontrolled Keywords:	MDA, Vitamin E, Aktivitas fisik submaksimal,MDA, vitamin E, submaximal physical activity
Subjects:	600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms
Divisions:	Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran
Depositing User:	Nur Cholis
Date Deposited:	19 Aug 2022 03:47
Last Modified:	19 Aug 2022 03:47
URI:	http://repository.ub.ac.id/id/eprint/193366

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