Studies of Biological Properties of Major Bioactive Compounds from the Stems of Paederia foetida. Linn

Ramadhan, Aryandi and Chi-I Chang, Ph.D. (2019) Studies of Biological Properties of Major Bioactive Compounds from the Stems of Paederia foetida. Linn. Magister thesis, Universitas Brawijaya.

Abstract

Paederia foetida Linn secara lokal dikenal sebagai skunk vine atau Chinese fever vine dari keluarga Rubiaceae. Secara tradisional, digunakan untuk mengobati enteromegali, radang usus, perut kembung, gastromegaly, rematik, rinitis, kopraemia, sakit, sakit perut dan sakit gigi. Dalam studi farmakologi modern, ekstrak P. foetida telah dilaporkan memiliki aktivitas antimikroba, antidiare, antiinflamasi, antispasmodik, anthelmintik, antitusif, dan hepatoprotektif.Dalam penelitian ini, ekstrak P. foetida diselidiki untuk konstituen bioaktif dan sifat farmakologis utamanya. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kandungan kimia batang P. foetida dan mengevaluasi aktivitas antibakterinya. Ekstrak metanol kasar dipartisi secara berurutan dengan etil asetat (EtOAc) dan n-butanol (n-BuOH)

English Abstract

Paederia foetida Linn locally known as skunk vine or Chinese fever vine from Rubiaceae Family. Traditionally, it is used to treat enteromegaly, enteritis, flatulence, gastromegaly, rheumatism, rhinitis, copraemia, sore, stomachache and toothache. In modern pharmacological studies, the extract of P. foetida has been reported to possess antimicrobial, antidiarrheal, anti-inflammatory, antispasmodic, anthelminthic, antitussive, and hepatoprotective activities. In this study, P. foetida extract was investigated for bioactive constituents and their main pharmacological properties. The research purpose was to isolate and identify the chemical constituents of the stem of P. foetida and evaluate their antibacterial activities. The crude methanol extract was sequentially partitioned with ethyl acetate (EtOAc) and n-butanol (n-BuOH). The EtOAc layer was further isolated and purified to obtain ten compounds including two steroids, four anthraquinones, two coumarins, and two aromatics. The compounds were identified using Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) analysis. The ten compounds were identified as β-sitosterol (S1), stigmastan-3-one (S2), morindaparvin A (An1), 1,3-dihydroxy-2-methoxyanthraquinone (An2), alizarin (An3), digiferrol (An4), scopoletin (C1), fraxidin (C2), ferulic acid (A1), and vanillic acid (A2). The antibacterial activity of the partitions and fractions were evaluated using agar well diffusion assay. The resazurin assay was used to determine the minimum inhibitory concentration (MIC). The result of agar diffusion assay showed that the EtOAc layer has significant inhibition against Escherichia coli (11 mm) and Staphylococcus aureus (11.5 mm) than the n-BuOH and water layer. S. aureus was very susceptible to, 1,3-dihydroxy-2-methoxyanthraquinone (An2), digiferrol (An4), and vanillic acid (A2) with MIC values ranging from 4.68-100 μg/mL. The EtOAc layer of methanol crude extract contains mainly anthraquinones as a dominant bioactive compound. The extract and fractions of P. foetida could be considered as prospective candidates for the prevention of bacterial infections.

Other obstract

Paederia foetida，俗稱雞屎藤，為茜草科植物。傳統上，它用於治療腸腫大、腸炎、胃腸脹氣、胃萎縮、風濕病、鼻炎、糞血症、疼痛、胃痛和牙痛。在現代藥理學研究中，據文獻指出P. foetida的萃取物具有抗菌、止瀉、抗發炎、抗痙攣、驅蟲、鎮咳和保肝活性。本研究目的為分離與鑑定P. foetida萃取物之化學成分，及探討其主要的藥理活性，並將具生物活性之化合物進行抗菌活性分析。P. foetida之甲醇粗萃物依序經由乙酸乙酯 (EtOAc) 及正丁醇 (n-BuOH) 進行分配萃取。將EtOAc層萃取物進一步分離純化，得到十個化合物，包含兩種固醇類、四種蒽醌類、兩種香豆素及兩種芳香類。化合物的的結構則是由核磁共振光譜 (Nuclear Magnetic Resonance , NMR) 及質譜 (Mass Spectrometry, MS) 來鑑定, 分別為β-sitosterol (S1)、stigmastan-3-one (S2)、 morindaparvin A (An1)、 1,3-dihydroxy-2-methoxyanthraquinone (An2)、 alizarin (An3)、 digiferrol (An4)、 scopoletin (C1)、fraxidin (C2)、 ferulic acid (A1) 與 vanillic acid (A2)。 P. foetida之分配萃取物及EtOAc層分液之抗菌活性將使用瓊脂擴散法分析，而刃天青還原試驗則用來做最小抑菌濃度 (minimum inhibitory concentration, MIC) 的確效。結果顯示EtOAc層比n-BuOH層及水層具有較顯著的抑制大腸桿菌 (11 mm) 及葡萄球菌 (11.5 mm) ，並發現1,3-dihydroxy-2-methoxyanthraquinone (An2) 、digiferrol (An4) 和 vanillic acid (A2) 具葡萄球菌之抑制活性，其MIC為4.68-100 μg/mL。P. foetida甲醇粗萃物的EtOAc層富含蒽醌類化合物，為抗菌的活性成分，因此其萃取物及分液具有抗菌感染的潛力。

Item Type:	Thesis (Magister)
Identification Number:	TES/615.323 93/RAM/s/2019/041901730
Uncontrolled Keywords:	Paederia foetida Linn, isolation, purification, spectroscopic analysis, bioactive properties
Subjects:	600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 95 Drugs derived from spesific plant (Scrophulariales)
Divisions:	S2/S3 > Magister Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian
Depositing User:	soegeng sugeng
Date Deposited:	12 Aug 2022 01:33
Last Modified:	12 Aug 2022 01:33
URI:	http://repository.ub.ac.id/id/eprint/193183

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