Nugraeni, Maria Gloria Falin and Prof. Ir. Liliek Sulistyowati, Ph.D and Luqman Qurata Aini, SP, MP., PhD (2022) Potensi Antagonis Jamur Rizosfer Pada Tanaman Kacang Hijau (Vigna Radiata L.) Dalam Mengendalikan Patogen Sclerotium Rolfsii. Sarjana thesis, Universitas Brawijaya.
Abstract
Kacang hijau (Vigna radiata L.) termasuk tanaman kacang- kacangan terpenting di Indonesia. Salah satu penyakit yang dapat menurunkan produktivitas kacang hijau yaitu penyakit busuk pangkal batang yang disebabkan oleh jamur Sclerotium rolfsii. Pengendalian penyakit yang ramah lingkungan dapat dilakukan dengan menggunakan jamur rizosfer. Tujuan penelitian ini adalah untuk mengetahui jamur rizosfer yang terdapat pada tanaman kacang hijau serta potensi antagonismenya dalam menghambat pertumbuhan jamur S. rolfsii penyebab penyakit busuk batang pada tanaman kacang hijau. Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya, Malang. Penelitian dilaksanakan pada bulan Maret 2021 – Agustus 2021. Penelitian ini dimulai dengan melakukan persiapan penelitian berupa sterilisasi alat dan pembuatan media Potato Dextrose Agar (PDA). Sedangkan pelaksanaan penelitian meliputi isolasi jamur patogen S. rolfsii, purifikasi, identifikasi isolat jamur S. rolfsii, eksplorasi jamur rizosfer, isolasi jamur rizosfer, purifikasi, pembuatan preparat jamur, pengamatan dan identifikasi jamur rizosfer dan uji antagonis jamur rizosfer terhadap jamur S. rolfsii. Penelitian dilakukan secara in vitro menggunakan rancangan acak lengkap dengan 34 perlakuan. Perlakuan terdiri dari kontrol dan 33 jenis jamur rizosfer yang berbeda sebagai perlakuan. Setiap perlakuan diulang 3 kali. Pengamatan dilakukan setiap hari selama 7 hari dengan mengukur jari-jari koloni patogen. Pengujian jamur antagonis dilakukan dengan metode oposisi langsung. Metode analisis yang digunakan adalah analisis ragam ((ANOVA) dan apabila terdapat perbedaan antar perlakuan, maka dilakukan uji lanjut Duncan taraf 5%. Hasil eksplorasi jamur rizosfer tanaman kacang hijau didapatkan jamur rizosfer sebanyak 33 isolat. Sebanyak 11 teridentifikasi dengan genus Penicillium, Trichoderma, Aspergillus, Gliocladium, Chrysonilia. Berdasarkan uji antagonis, didapatkan 13 isolat yang berpotensi sebagai jamur antagonis. Isolat tersebut yaitu Penicillium sp.B1.1, Penicillium sp.B1.2, isolat B2.1, Trichoderma sp. B2.2, Aspergillus sp. B2.5, Gliocladium sp. K1.3, Aspergillus sp. K2.5, Gliocladium sp. KR5, Penicillium sp. KR6, Chrysonilia sp. KR8, Aspergillus sp. KR9, Trichoderma sp. S1, isolat S2. an presentase daya antagonis tertinggi pada isolat Trichoderma sp.S1
English Abstract
Mung bean (Vigna radiata L.) is one of most important legume crops in Indonesia. One of the diseases that can decrease mung bean productivity is the stem rotten disease caused by the fungi Sclerotium rolfsii . Control environmentally friendly disease can be done by using rizhosphere fungi. The purpose of this research are to know the rizhosphere fungi of mung bean plants and to know potentially antagonistic and most effectively suppresses the growth of S. rolfsii that cause stem rot disease in mung bean plants. This research was conducted in the Plant Disease Laboratory, Department of Pests and Disease, Faculty of Agriculture, University of Brawijaya, Malang from March 2021 until August 2021. This research began with the preparation of research in the form of sterilization of tools and making media Potato Dextrose Agar (PDA). While the implementation of this research include the isolation of pathogenic of S. rolfsii, purification, identification of S. rolfsii, exploration of rhizosphere fungi, isolation of rizhosphere fungi, purification, manufacture of fungi preparations, observation and identification of rhizosphere fungi and the test of rizhosphere fungi antagonist against S. rolfsii. The experiment methodes used a complete randomized design with 34 treatments. The treatments consists of a control and 33 different rizhosphere on each treatment in vitro. Every treatment repeatedly 3 times. The observation carried out every day during 7 days by measuring the radius of pathogen. The testing of antagonistic fungi is done by direct opposition methods. The analysis method used the analysis of variance (Anova). And if there are differences between treatments, then a further Duncan test of 5% would be carried out. The results of exploration from mung bean plants obtained as many as 33 isolates of rhizosphere. The genus that has been identified from 11 isolates were Penicillium, Trichoderma, Aspergillus, Gliocladium, Chrysonilia. The result from antagonist test, there are 13 isolates that have potential as antagonist fungi. Penicillium sp.B1.1, Penicillium sp.B1.2, isolate B2.1, Trichoderma sp. B2.2, Aspergillus sp. B2.5, Gliocladium sp. K1.3, Aspergillus sp. K2.5, Gliocladium sp. KR5, Penicillium sp. KR6, Chrysonilia sp. KR8, Aspergillus sp. KR9, Trichoderma sp. S1, isolate S2. The highest presentation of antagonist test are from Trichoderma sp.S1
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522040068 |
| Subjects: | 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.6 Animal pests |
| Divisions: | Fakultas Pertanian > Hama dan Penyakit Tanaman |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2022 06:39 |
| Last Modified: | 19 Jul 2022 06:39 |
| URI: | http://repository.ub.ac.id/id/eprint/192303 |
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