Rahmawati, Vatien (2018) Peranan Extracellular Signal-Regulated Kinase (ERK) 1/2 Inhibitor dalam Menurunkan Pericyte Loss pada Tikus Model Diabetes. Magister thesis, Universitas Brawijaya.
Abstract
Tujuan: Mengetahui peranan ERK 1/2 inhibitor terhadap perisit retina pada tikus model diabetes melalui penghambatan jalur ERK 1/2. Metode: Desain penelitian true experimental in vivo di Laboratorium Biosains Universitas Brawijaya antara September 2018 - Desember 2018. Sampel penelitian tikus wistar jantan usia 6 minggu, 150-200 gram yang dinduksi diabetes dengan streptozotocin 40 mg/kgBB intraperitoneal. Setelah diabetes setiap sampel diberi ERK 1/2 inhibitor intravitreal (U0126) dengan dosis kelompok I 0,05 mM, kelompok II 0,10 mM, kelompok III 0,15 mM. Tikus kemudian dienukleasi dan dilakukan proses retinal digest pada mata tikus dilanjutkan pewarnaan hematoxylin. Rasio perisit:endotel diamati untuk menilai adanya perycite loss, kemudian dianalisa secara statistik. Hasil: Setelah 6 minggu mengidap diabetes didapatkan rasio perisit: endotel kontrol negatif adalah 1:1,40, kelompok I adalah 1: 1,50, kelompok II adalah 1:1,76, kelompok III adalah 1:2,10, dan kontrol positif adalah 1:3,10. Hasil uji one way anova didapatkan nilai signifikasi sebesar 0,036 (p<0,05) sehingga dinyatakan terdapat perbedaan antar kelompok perlakuan yang diuji. Hasil uji tukey didapatkan perbedaan tidak signifikan antara kelompok kontrol negatif dan semua kelompok perlakuan (p>0,05), menunjukkan dengan pemberikan ERK 1/2 inhibitor terjadi penurunan pericyte loss yang mendekati normal. Sedangkan antara kelompok pertama dengan kontrol negatif menunjukkan perbedaan signifikan (p 0,020). Hasil uji regresi linier menunjukkan tidak perbedaan yang signifikan antara besar dosis ERK 1/2 inhibitor terhadap rasio perisit:endotel. Kesimpulan: Terdapat penurunan pericyte loss pada semua kelompok perlakuan. Kelompok dengan dosis ERK 1/2 inhibitor 0,05mM menunjukkan hasil terbaik dalam menurunkan pericyte loss.
English Abstract
Aim: To know the role of ERK 1/2 inhibitor to retinal pericyte cell on diabetic rat through ERK 1/2 pathway inhibition. Methods: Study design true experimental in vivo at Biosains Laboratory Universitas Brawijaya on September 2018 – Desember 2018. Male wistar rat samples, 6 weeks, 150-200 grams had diabetic induced by streptozotocin 40 mg/kg BW intraperitoneal. After diabetes achieved each samples were given ERK 1/2 inhibitor intravitreal (U0126) with dosage: group I 0,05 mM, group II 0,10 mM, group III 0,15 mM. Rats were enucleated and through retinal digest process followed by hematoxylin staining. Pericyte: endothel ratio were observed to determined perycite loss, the result then statistically analyzed. Result: The ratio of pericyte: endothel for negative control is 1;1.40, group I is 1: 1.50, group II is 1:1.76, group III is 1:2.10, and positive control is 1:3.10. One way anova test showed significance value 0.036 (p<0.05) stated there are difference between tested sample groups. Tukey test showed no significance value (p>0.05) between control negative and all treated groups which showed that there is decrease in pericyte loss closed to normal level. While there is significant difference between first group and control negatif (p 0,020)Linier regression test showed no significant difference between ERK 1/2 inhibitor dosage and pericyte: endothel ratio. Conclusion: There are decrease in pericyte loss on all treated groups, group that received 0.05mM ERK 1/2 inhibitor showed the best in decreasing pericyte loss.
Other obstract
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| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/616.462/RAH/p/2018/041901324 |
| Uncontrolled Keywords: | Extracellular-Signal Regulated Kinase (ERK) 1/2 inhibitor, U0126, ,tikus model diabetes, pericyte loss |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.4 Diseases of endocrine, hematopoietic, lymphatic, glandular system; diseases of male breast > 616.46 Diseases of islands of Langerhans > 616.462 Diabetes mellitus |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Kesehatan Mata, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 20 Jun 2022 08:06 |
| Last Modified: | 20 Jun 2022 08:06 |
| URI: | http://repository.ub.ac.id/id/eprint/191299 |
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