Perbedaan Pemberian Gel Ekstrak Buah Ciplukan (Physalis angulate L.) dengan Triamcinolone Acetonide Terhadap Ekspresi PECAM-1 (Platelet Endothelial Cell Adhession Molecule-1) dan Penutupan Luka pada Proses Penyembuhan Ulkus Traumatikus Mukosa Labial Tikus Putih (Rattus Norvegicus)

Febrianti, Dwi Ervina and Dr. drg. Nur Permatasari,, MS. (2020) Perbedaan Pemberian Gel Ekstrak Buah Ciplukan (Physalis angulate L.) dengan Triamcinolone Acetonide Terhadap Ekspresi PECAM-1 (Platelet Endothelial Cell Adhession Molecule-1) dan Penutupan Luka pada Proses Penyembuhan Ulkus Traumatikus Mukosa Labial Tikus Putih (Rattus Norvegicus). Sarjana thesis, Universitas Brawijaya.

Abstract

Latar Belakang: PECAM-1 (Platelet Endothelial Adhesion Molecule-1) merupakan molekul adhesi sel dan mediator interaksi, baik antara leukosit dan endotelial maupun endotelial dan endothelial. PECAM-1 berperan penting dalam proses angiogenesis penyembuhan luka. Antibodi PECAM-1 mencirikan respon neoangiogenik luka pada tikus yang dilakukan dengan immunostaining penanda sel endotel PECAM-1 pada luka selama proses penyembuhan. Gel Ekstrak buah ciplukan mengandung senyawa aktif lupeol mampu meningkatkan promosi luka penutupan luka dengan menginduksi jaringan granulasi dan meningkatkan re-epitelisasi, β-sitosterol memiliki efek pada angiogenesis dengan cara mempengaruhi sel endotel. Tujuan penelitian ini adalah untuk mengetahui apakah terdapat perbedaan pemberian Gel ekstrak buah ciplukan (Physalis angulata L.) dengan triamcinolone acetonide terhadap ekspresi PECAM-1 dan penutupan luka pada penyembuhan luka ulkus traumatikus mukosa labial tikus putih. Metode yang digunakan adalah true experimental secara Randomized Post test Only Control Group Design secara in vivo, penelitian ini dibagi menjadi 9 kelompok dengan 3 time series yaitu hari ke-2, ke6 dan ke12 kelompok kontrol, Physalis dan triamcinolone setelah terbentuknya ulkus traumatikus. Tahap selanjutnya pengambilan jaringan ulkus traumatikus, pewarnaan HE dan IHK, kemudian ekspresi PECAM-1 diamati menggunakan mikroskop cahaya dilengkapi kamera digital dan penutupan luka diamati setiap hari dan didokumentasikan. Hasil uji independent t-test didapatkan perbedaan yang signifikan pada kelompok physalis dan triamcinolone pada hari ke-2, ke-6 dan ke-12. Hasil uji korelasi pada kelompok physalis didapatkan semakin lama perlakuan dapat meningkatkan ekpresi PECAM-1 dan penutupan luka, pada kelompok triamcinolone semakin lama perlakuan dapat menurunkan ekpresi PECAM-1. Berdasarkan hasil penelitian, dapat disimpulkan bahwa terdapat perbedaan pemberian gel ekstrak buah cipluka dengan triamcinolone acetonide terhadap ekspresi PECAM-1 dan penutupan luka pada proses penyembuhan luka ulkus traumatikus mukosa labial tikus putih.

English Abstract

Background: PECAM-1 (Platelet Endothelial Adhesion Molecule-1) is a cell adhesion molecule and mediator of interaction, both between leukocytes and endothelial as well as endothelial and endothelial. PECAM-1 plays an important role in the process of wound healing angiogenesis. PECAM-1 antibodies characterize the neoangiogenic response of wounds in rats performed by immunostaining PECAM-1 endothelial cell markers in wounds during the healing process. Ciplukan fruit extract gel containing lupeol active compounds can increase wound closure promotion by inducing granulation tissue and increasing re-epithelialization, β-sitosterol has an effect on angiogenesis by influencing endothelial cells. The purpose of this study was to determine whether there were differences in the administration of ciplukan (Physalis angulata L.) Gel Gel extract with triamcinolone acetonide on PECAM-1 expression and wound closure in wound healing of white rat mucous traumatic ulcer traumatic ulcer. The method used was true experimental randomized post test only control group design in vivo, this study was divided into 9 groups with 3 time series, namely the 2nd, 6th and 12th days of the control group, Physalis and Triamcinolone after the formation of traumatic ulcers. The next stage is the removal of traumatic ulcer tissue, HE and IHK staining, then PECAM-1 expression is observed using a light microscope equipped with a digital camera and wound closure is observed daily and documented. The results of the independent t-test showed significant differences in the physalis and triamcinolone groups on the 2nd, 6th and 12th days. Correlation test results in the physalis group showed that the longer the treatment could increase PECAM-1 expression and wound closure, the triamcinolone group the longer the treatment could reduce PECAM-1 expression. Based on the results of the study, it can be concluded that there are differences in the administration of cipluka fruit extract gel with triamcinolone acetonide on the expression of PECAM-1 and wound closure in the healing process of traumatic mucosal traumatic ulcer wounds of white rats.

Item Type: Thesis (Sarjana)
Identification Number: 0520140024
Uncontrolled Keywords: Ulkus Traumatikus, PECAM-1, Penutupan Luka, Gel Ekstrak Buah Ciplukan (Physalis angulata L), Triamcnolone acetonide,Traumatic Ulcer, PECAM-1, Wound Closure, Morel Berry Fruit Extract (Physalis angulata L), Triamcnolone acetonide.
Subjects: 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry
Divisions: Fakultas Kedokteran Gigi > Kedokteran Gigi
Depositing User: soegeng sugeng
Date Deposited: 17 Jun 2022 01:16
Last Modified: 17 Jun 2022 01:16
URI: http://repository.ub.ac.id/id/eprint/191004
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