Aldina, Syafira and Prof. Dr. Ir. Nurul Isnaini, M,P (2022) Produksi Embrio Induk Donor Sapi Friesian Holstein pada Umur Berbeda. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini dilaksanakan pada 25 September- 15 November 2021 di Balai Embrio Ternak (BET) Cipelang, Bogor, Jawa Barat. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh umur yang berbeda dari sapi donor Friesian Holstein (FH) ditinjau dari lima variable yakni jumlah corpus luteum (CL), response rate, total perolehan embrio, kualitas embrio (123, DG, dan UF), dan recovery rate. Metode penelitian yang digunakan ialah studi kasus dengan teknik pengambilan data secara purposive sampling, teknik pemeliharaan kandang sapi donor menggunakan kandang free stall agar sapi dapat bergerak, makan dan minum dengan leluasa, dan diberikan pakan berupa rumput gajah (Pennisetum purpureum) sebanyak 30-40 kg/ekor/hari serta diberikan minum secara ad libitum (tidak terbatas). Teknik produksi embrio dilakukan secara in vivo dengan menggunakan Progesteron Internal Drug Release (PIDR). Materi dari penelitian ini adalah data produksi embrio 10 ekor sapi donor Friesian Holstein (FH) dari 2009-2020 dengan bobot badan 500 – 650 kg. Sapi donor Friesian Hosltein (FH) disuperovulasi dengan menggunakan hormone Follicle Stimulating Hormone (FSH) yang disuntikkan dengan dosis menurun 4 ml, 3 ml, 2 ml, dan 1 ml secara intra muskular dengan dosis total 400 mg/ 20 ml. Inseminasi Buatan (IB) dilakukan sebanyak 3 kali secara rekto vaginal. Pemanenan embrio dilakukan menggunakan metode non surgical (tanpa pembedahan) menggunakan media pembilasan berupa ringer lactate yang merupakan campuran dari Fetal Calf Serum (FCS) dan antibiotik 0,1% (Streptomycin dan Penicilin) Rangkaian produksi embrio sampai dengan proses flushing (pemanenan embrio) adalah 20 hari. Data yang diperoleh diolah dan dianalisis secara deskriptif serta disajikan dalam bentuk tabel dan grafik. Berdasarkan hasil penelitian bahwa untuk rataan jumlah corpus luteum (CL) tertinggi yaitu 10,89 ± 7,91 pada umur 5 tahun. Response rate tertinggi yaitu 100% pada umur 4-9 tahun. Rataan total perolehan embrio tertinggi yaitu 9,60 ± 12,07 pada sapi donor umur 7 tahun. Rataan embrio layak transfer tertinggi yaitu 5,53 ± 5,79 pada sapi donor umur 4 tahun. Rataan embrio tidak layak transfer terendah untuk embrio degeneratif (DG) yaitu 0,60 ± 0,83 dan untuk embrio unfertile (UF) terendah yaitu 0,67 ± 1,80 pada umur 4 tahun. Recovery rate tertinggi yaitu. 82% pada umur 7 tahun. Dari hasil penelitian disimpulkan bahwa sapi donor FH umur 4 tahun adalah umur yang optimal dalam menghasilkan embrio yang maksimal dari segi jumlah corpus luteum (CL), total perolehan embrio, response rate, kualitas embrio dan recovery rate.
English Abstract
This research was conducted from September 25 to November 15 2021 at the Cipelang Livestock Embryo Center (BET), Bogor, West Java. The purpose of this study was to determine the effect of different ages of Friesian Holstein (FH) donor cows in terms of five variables, namely the number of corpus luteum (CL), response rate, total embryo acquisition, embryo quality (123, DG, and UF), and recovery rate. The research method is a case study with purposive sampling data collection techniques, the technique of maintaining donor cows is using a free stall cage so that the cows can move, eat and drink freely, and are given feed in the form of elephant grass (Pennisetum purpureum) as much as 30-40 kg/head/ day and given to drink ad libitum (unlimited). The embryo production technique was carried out in vivo using Progesterone Internal Drug Release (PIDR). The material of this research is the embryo production data of 10 Friesian Holstein (FH) donor cows from 2009-2020 with a body weight of 500-650 kg. Friesian Hosltein (FH) donor cows were superovulated using Follicle Stimulating Hormone (FSH) hormone which was injected in decreased doses of 4 ml, 3 ml, 2 ml, and 1 ml intramuscularly with a total dose of 400 mg/20 ml. Artificial Insemination (IB) was performed 3 times recto vaginally. Harvesting of embryos was carried out using a non-surgical method (without surgery) using flushing media in the form of Ringer's lactate which is a mixture of Fetal Calf Serum (FBS) and 0.1% antibiotics (Streptomycin and Penicillin). The data obtained were processed and analyzed descriptively and presented in the form of tables and graphs. Based on the results of the study, the highest average number of corpus luteum (CL) was 10.89 ± 7.91 at the age of 5 years. The highest response rate is 100% at the age of 4-9 years. The highest mean total embryo acquisition was 9.60 ± 12.07 in donor cows aged 7 years. The highest mean of transferable embryos was 5.53 ± 5.79 in donor cows aged 4 years. The lowest mean of unfit embryo transfer for degenerative embryos (DG) was 0.60 ± 0.83 and for unfertile embryos (UF) the lowest was 0.67 ± 1.80 at the age of 4 years. The highest recovery rate ie. 82% at the age of 7 years. From all data can be concluded that FH donor cows aged 4 years are the optimal age in producing maximum embryos in terms of the number of corpus luteum (CL), total embryo acquisition, response rate, embryo quality and recovery rate. It is necessary to conduct further studies regarding the effect of the age of the Friesian Holstein (FH) donor cow on the number of corpus luteum, especially the minimum standard determining the number of corpus luteum produced.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0522050014 |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry |
| Divisions: | Fakultas Peternakan > Peternakan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 26 Apr 2022 03:51 |
| Last Modified: | 26 Apr 2022 03:51 |
| URI: | http://repository.ub.ac.id/id/eprint/190178 |
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