Sintesis dan Uji Aktivitas Antibakteri Senyawa 1- alil-3-benzoiltiourea secara In Vitro Terhadap Salmonella Typhi Isolat Darah

Rahman, Hanif Syafa’atur and Prof. Dr.apt. Sri Winarsih, M. Si.,Dra., and apt. Alvan Febrian Shalas, S. Farm., M. Farm. (2021) Sintesis dan Uji Aktivitas Antibakteri Senyawa 1- alil-3-benzoiltiourea secara In Vitro Terhadap Salmonella Typhi Isolat Darah. Sarjana thesis, Universitas Brawijaya.

Abstract

Meningkatnya angka kejadian resistensi antibakteri khususnya dalam pengobatan demam tifoid melatarbelakangi untuk dilakukan penelitian mengenai pengembangan senyawa baru yang memiliki aktivitas antibakteri. Tujuan penelitian ini adalah memperoleh senyawa baru 1-alil-3-benzoiltiourea yang diharapkan memiliki aktivitas antibakteri terhadap Salmonella Typhi isolat klinis (darah). Penemuan senyawa 1-alil-3-benzoiltiourea dilakukan melalui metode sintesis Schotten-Baumann dengan reaksi substitusi nukleofilik, sedangkan uji aktivitas antibakteri menggunakan metode dilusi agar dengan parameter Kadar Hambat Minimum (KHM). Konsentrasi senyawa uji 1-alil-3-benzoiltiourea yang digunakan yaitu 1000 ppm; 500 ppm; 250 ppm; 125 ppm; 62,5 ppm; 31,25 ppm; 15,625 ppm; dan 7,8125 ppm. Hasil penelitian menunjukkan bahwa senyawa 1- alil-3-benzoiltiourea dapat disinteis melalui metode Schotten-Baumann dengan reaksi subsitusi nukleofilik. Hasil sintesis dikonfirmasi menggunakan Spektrofotometri Ultraviolet (UV), Spektrofotometri Inframerah (IR), Spektrometri Resonansi Magnet Inti (NMR), dan Spektroskopi Massa. Hasil uji aktivitas antibakteri senyawa 1-alil-3-benzoiltiourea menunjukkan tidak didapatkan nilai KHM (bakteri tumbuh semua pada semua konsentrasi senyawa uji). Oleh karena itu, disimpulkan bahwa senyawa 1-alil-3-benzoiltiourea hasil sintesis tidak memiliki aktivtas antibakteri terhadap Salmonella Typhi isolat klinis (darah).

English Abstract

The increasing incidence of antibacterial resistance, especially in the treatment of typhoid fever, is the background for researching the development of new compounds that have antibacterial activity. The purpose of this study was to obtain a new compound 1-allyl-3-benzoylthiourea which is expected to have antibacterial activity against clinical isolates of Salmonella Typhi (blood). The discovery of the compound 1-allyl-3-benzoylthiourea was carried out through the Schotten- Baumann synthesis method with a nucleophilic substitution reaction, while the antibacterial activity test used the agar dilution method with the parameter of Minimum Inhibitory Concentration (MIC). The concentration of the test compound 1-allyl-3-benzoylthiourea used was 1000 ppm; 500 ppm; 250 ppm; 125 ppm; 62.5 ppm; 31.25 ppm; 15.625 ppm; and 7.8125 ppm. The results showed that the compound 1-allyl-3-benzoylthiourea could be synthesized through the Schotten- Baumann method with a nucleophilic substitution reaction. The synthesis results were confirmed using Ultraviolet (UV) Spectrophotometry, Infrared (IR) Spectrophotometry, Core Magnetic Resonance Spectrometry (NMR), and Mass Spectroscopy. The results of the antibacterial activity test of 1-allyl-3- benzoylthiourea showed no MIC value (bacteria grew all at all concentrations of the test compound). Therefore, it was concluded that the synthetic compound 1- allyl-3-benzoylthiourea did not have antibacterial activity against clinical isolates of Salmonella Typhi (blood).

Item Type: Thesis (Sarjana)
Identification Number: 0521060143
Uncontrolled Keywords: Sintesis, Senyawa 1-alil-3-benzoiltiourea, Schotten-Baumann, dilusi agar, Kadar Hambar miminum (KHM). Synthesis, 1-allyl-3-benzoylthiourea compound, Schotten-Baumann, Agar Dilution, Minimum Inhibitory Concentration (MIC)
Subjects: 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.1 Drugs (materia medica)
Divisions: Fakultas Kedokteran > Farmasi
Depositing User: Budi Wahyono Wahyono
Date Deposited: 20 Dec 2021 03:16
Last Modified: 24 Feb 2022 01:25
URI: http://repository.ub.ac.id/id/eprint/187495
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