Association and Potential Genetic Superiority Identification of Madura Bulls Based on Single Nucleotide Polymorphisms identification of Inhibin and IGF1 Genes

Novianti, Irida (2021) Association and Potential Genetic Superiority Identification of Madura Bulls Based on Single Nucleotide Polymorphisms identification of Inhibin and IGF1 Genes. Doctor thesis, Universitas Brawijaya.

Abstract

" Madura is an Indonesian indigenous cattle breed that has some superior traits and important functions for Madura farmers. Therefore, efforts to improve productivity of Madura cattle are necessary. Selection of Madura bulls based on both phenotypic and molecular information are some efforts that can be implemented. Inhibin A (INHA), inhibin BA (INHBA) and insulin-like growth factor 1 (IGF1) are three candidate genes for exterior performance and reproduction traits that were explored in this study. The aims of this study were to investigate polymorphisms of the IGF1, INHA and INHBA genes in Madura bulls, to assess the association between identified polymorphisms/markers of the IGF1 and inhibin genes (INHA and INHBA) with semen production traits and body dimension traits related to body conformation of the Madura bull, and to identify and compare the potential genetic superiority (based on identified SNPs) of Madura bulls at SNAIC and Pamekasan, Madura. Semen and DNA samples from 58 and 5 Madura bulls obtained from Pamekasan region and Singosari National Artificial Insemination Centre (SNAIC), respectively, were used. Body measurements, semen production traits assessment, single nucleotide polymorphisms (SNP) identification and association studies between identified SNPs and the observed traits were conducted. Results showed that there is only one SNP for INHA (INHA SNP4) had significant effects on sperm viability in the Pamekasan bull population. There were significant association indicated between a SNP for INHA (INHA SNP3) and IGF1 SNP with chest girth, body height and hip height. INHA SNP1, INHA SNP2 and INHA SNP4 are the most interesting SNPs since there is only one bull (Adikara) at SNAIC carrying the favorable genotype for those SNPs. The results of this study might be the starting point to select for superior Madura bulls at Pamekasan to select for bulls similar to Adikara in phenotype using genotyping. Keywords: INHA, IGF1, SNPs, Madura bulls, selection"

Item Type:	Thesis (Doctor)
Uncontrolled Keywords:	INHA, IGF1, SNPs, Madura bulls, selection
Subjects:	600 Technology (Applied sciences) > 636 Animal husbandry
Divisions:	S2/S3 > Doktor Ilmu Ternak, Fakultas Peternakan
Depositing User:	Unnamed user with username verry
Date Deposited:	22 Oct 2021 03:21
Last Modified:	24 Feb 2022 01:38
URI:	http://repository.ub.ac.id/id/eprint/184908

Text (DALAM MASA EMBARGO) Disertasi_FAPET_ Irida Novianti, S. Pt.pdf Restricted to Registered users only until 31 December 2023. Download (2MB)

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