Putri, Kania Salsabila (2020) Pengaruh Jenis Pelarut Terhadap Daya Antibakteri Hasil Ekstraksi Daun Sirih Hijau Pada Aktivitas Staphylococcus Aureus. Sarjana thesis, Universitas Brawijaya.
Abstract
Daun sirih hijau diduga memiliki senyawa aktif yang bersifat antibakteri berupa senyawa fenol dan terpene beserta turunannya. Senyawa aktif dari daun sirih hijau ini dapat diperoleh dengan proses ekstraksi. Daun sirih hijau diekstraksi menggunakan metode ekstraksi refluks pada suhu operasi 65oC selama 4 jam dengan tiga jenis pelarut dengan kepolaran yang berbeda yaitu etanol yang bersifat polar, etil asetat bersifat semi polar, dan heksana yang bersifat non polar. Ekstrak daun sirih hijau menggunakan berbagai pelarut tersebut kemudian dilakukan pengujian daya antibakteri terhadap pertumbuhan bakteri staphylococcus aureus menggunakan metode difusi. Hasil penelitian ini menunjukkan bahwa rendemen terbesar dimiliki oleh ekstrak daun sirih dengan pelarut etanol (EDS-E) sebesar (5,77%) diikuti oleh etil asetat (EDS-EA) sebesar (4,26%), dan heksana (EDS-H) sebesar (1,55%). Kadar fenol sampel EDS-E, EDS-EA, dan EDS-H secara berturut-turut sebesar (5,17%), (2,22%) dan (8,45%). Hasil uji daya antibakteri diamati pada media uji antibakteri EDS-E, EDS-EA, dan EDS-H secara berturut-turut terbentuk diameter zona terang sebesar (19 mm), (22 mm) dan (17 mm) dan hasil pengujian kualitatif saponin menunjukkan untuk EDS-E dan EDS-EA positif dan hasil negatif pada EDS-H. Uji kualitatif saponin dinyatakan positif dengan terbentuknya busa pada permukaan ekstrak. Kemudian untuk hasil pengujian kualitatif tanin menunjukkan untuk EDS-E, EDS-EA dan EDS-H seluruhnya positif yang ditunjukkan dengan perubahan warna menjadi biru atau hijau kehitaman. Berdasarkan hasil tersebut dapat disimpulkan bahwa daya antibakteri EDS-EA merupakan yang paling kuat di bandingkan EDS-E dan EDS-H serta diketahui senyawa antibakteri dalam ekstrak daun sirih hijau yang berperan penting dalam penghambatan pertumbuhan bakteri s.aureus yaitu senyawa aktif saponin serta didukung oleh senyawa antibakteri lainnya yaitu fenol dan tanin.
English Abstract
Green betel leaf is thought to have an active compound that is antibacterial in the form of phenol and terpene compounds and their derivatives. The active compound of green betel leaf can be obtained by extraction process. Green betel leaf was extracted using the reflux extraction method at an operating temperature of 65oC for 4 hours with three types of solvents with different polarity, namely ethanol which is polar, ethyl acetate is semi-polar, and non-polar hexane. Green betel leaf extract using various solvents was then tested for antibacterial power against the growth of staphylococcus aureus bacteria using the diffusion method. The results of this study indicate that the largest yield is owned by betel leaf extract with ethanol solvent (5.77%) followed by ethyl acetate (4.26%), and hexane (1.55%). Phenol levels were obtained from betel leaf extract with each ethanol, ethyl acetate and hexane (5.17%), (2.22%) and (8.45%) solvents. Antibacterial test results were observed on the antibacterial test media that were given betel leaf extract with ethanol, ethyl acetate and hexane solvents which formed the diameter of clear zones (19 mm), (22 mm) and (17 mm) as well as qualitative results of saponin testing showed for betel leaf extract with positive ethanol and ethyl acetate solvents and negative results on betel leaf extract with hexane solvent. The saponin qualitative test was stated positive by forming foam on the surface of the extract. Then for the results of qualitative tannin testing showed that betel leaf extract with ethanol, ethyl acetate and hexane solvents were all positive as indicated by the change in color to blue or blackish green. Based on these results it can be concluded that the antibacterial power of betel leaf extract with ethyl acetate solvent is the most powerful compared to using ethanol and peer hexane and it is known that the antibacterial compound in green betel leaf extract which plays an important role in inhibiting the growth of staphylococcus aureus is the active compound saponin and is supported by other antibacterial compounds namely phenols and tannins.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520070146 |
| Uncontrolled Keywords: | antibakteri, daun sirih, ekstraksi, pelarut, s.aureus. , antibacterial, extraction, green betel leaf, solvent, staphylococcus aureus. |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes |
| Divisions: | Fakultas Teknik > Teknik Kimia |
| Depositing User: | Bambang Septiawan |
| Date Deposited: | 23 Feb 2021 06:39 |
| Last Modified: | 12 Jan 2023 04:43 |
| URI: | http://repository.ub.ac.id/id/eprint/182689 |
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Text (DALAM MASA EMBARGO)
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