Ralahallo, Eraldy Alessandro (2020) Efektivitas Kombinasi Ekstrak Buah Mengkudu (Morinda citrifolia) dan Daun Tapak Dara (Catharanthus roseus) terhadap Jumlah Relatif Sel T CD4+ TNF-α+ dan Sel T CD4+ IFN-γ+ pada Mencit (Mus musculus) Model Kanker Payudara. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk menganalisis efektivitas pemberian kombinasi ekstrak buah mengkudu dan daun tapak dara (MTD) terhadap jumlah relatif sel T CD4+ TNF-α+ dan CD4+ IFN-γ+ pada mencit model kanker payudara. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan enam kelompok perlakuan, yaitu mencit normal (N), mencit kanker payudara (K), mencit kanker payudara dengan perlakuan cisplatin 5 mg/kg BB (C), mencit kanker payudara dengan perlakuan ekstrak kombinasi buah mengkudu 200 mg/kg BB dan daun tapak dara 15 mg/kg BB (D1), perlakuan ekstrak kombinasi buah mengkudu 1000 mg/kg BB dan daun tapak dara 75 mg/kg BB (D2), dan perlakuan ekstrak kombinasi buah mengkudu 1000 mg/kg BB dan daun tapak dara 375 mg/kg BB (D3). Induksi kanker dilakukan pada mencit betina berusia 6 minggu menggunakan DMBA di sekitar areola. Pemberian herbal dilakukan selama 7 hari menggunakan teknik sonde, setelahnya dilakukan isolasi spleen. Analisis jumlah relatif sel T CD4+ TNF-α+ dan CD4+ IFN-γ+ dilakukan menggunakan flow cytometry. Data dianalisis menggunakan uji statistik One Way ANOVA dengan uji lanjut Tuckey HSD (α=5%). Pemberian MTD menunjukkan penurunan jumlah relatif sel T CD4+ TNF-α+ dan CD4+ IFN-γ+ pada seluruh kelompok perlakuan. Hasil penelitian ini mengindikasikan adanya potensi penggunaan herbal MTD sebagai alternatif pengobatan kanker payudara dan D1 memberikan hasil terbaik.
English Abstract
The aim of this research is to analyze effectivity of combination of noni fruit and periwinkle leaves (MTD) extract to the relative amount of CD4+ TNF-α+ and CD4+ IFN-γ+ T cells in mice with breast cancer models. This study used a Completely Randomized Design (CRD) with six treatment groups, which are: normal mice (N), breast cancer mice (K), breast cancer mice with Cisplatin treatment of 5 mg/kg BW (C), breast cancer mice which treated with combination of 200 mg/kg BW of noni fruit and 15 mg/kg BW of periwinkle leaf extract (D1), breast cancer mice which treated with combination of 1000 mg/kg BW of noni fruit and 75 mg/kg BW of periwinkle leaf extract (D2), and breast cancer mice which treated with combination of 5000 mg/kg BW of noni fruit and 375 mg/kg BW of periwinkle leaf extract (D3). Breast cancer induction was performed on 6 weeks old female mice using DMBA injection around the areola. Herbal treatment was given in 7 days using oral gavage technique, than the spleen isolation was performed. Relative amount analysis of CD4+ TNF-α+ and CD4+ IFN-γ+ T cells was performed using flow cytometry. Data were analyzed using One Way ANOVA and continued with Tuckey HSD test (α=5%). MTD process showed a decrease in the relative amount of CD4+ TNF-α+ and CD4+ IFN-γ+ in all treatment groups. The results of this research indicate the potential use of MTD herbal as an alternative treatment for breast cancer and D1 shown the best result.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | 0520090061 |
| Uncontrolled Keywords: | Catharanthus roseus, Sel T CD4, IFN-γ, Morinda citrifolia, TNF-α |
| Subjects: | 500 Natural sciences and mathematics > 583 Magnoliopsida (Dicotyledons) > 583.6 Dilleniidae > 583.64 Capparales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | ismanto |
| Date Deposited: | 31 Jan 2021 16:32 |
| Last Modified: | 11 Jul 2022 07:21 |
| URI: | http://repository.ub.ac.id/id/eprint/182289 |
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Text (DALAM MASA EMBARGO)
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