Karunia, Nia (2020) Identifikasi Abnormalitas Gen CD18 pada Populasi Pedet Peranakan Friesian Holstein (PFH) dengan Metode PCR-RFLP di Kecamatan Batu dan Bumiaji, Kota Batu. Sarjana thesis, Universitas Brawijaya.
Abstract
Kelainan genetik merupakan salah satu kelainan pada ternak yang disebabkan oleh mutasi pada material genetik. Alel yang cacat dapat bersifat spesifik untuk breed tertentu seperti pada penyakit Bovine Leukocyte Adhesion Deficiency (BLAD) menyerang ras Holstein. Mutasi pada gen CD18 dapat mengindikasikan adanya BLAD pada sapi perah Friesian Holstein dan peranakannya. Tujuan dari penelitian ini adalah mengetahui apakah metode PCR-RFLP dapat mengidentifikasi abnormalitas gen CD18 pada pedet Peranakan Friesian Holstein (PFH) dan mengetahui cara perhitungan frekuensi alel sub unit CD18 yang menyebabkab BLAD. Penelitian ini menggunakan 14 sampel yang diperoleh dari populasi pedet PFH yang terdapat di Kecamatan Batu dan Bumiaji. Amplifikasi DNA dilakukan dengan metode Polymerase Chain Reaction menggunakan forward primer 5´-TCA ACG TGA CCT TCC GGA GG-3´ dan reverse primer 5´-CCC AGA TTC TTG ACG TTG AC-3´. Deteksi mutasi gen CD18 dilakukan melalui teknik RFLP (Restriction Fragment Length Polymorphism) menggunakan enzim restriksi XbaI. Pada hasil elektroforesis agarosa 2 % sampel nomor 1-3 dan 9-14 yang telah di RFLP menunjukkan tiga potongan pita yang berarti sampel bersifat heterozigot bergenotip Dd. Sementara sampel nomor 4-8 menunjukkan dua potongan pita yang berarti sampel bersifat homozigot dominan bergenotip DD, dan tidak ditemukan adanya pita homozigot resesif yang bergenotip dd. Mutasi Gen CD18 pada populasi pedet Peranakan Friesien Holstein di Kecamatan Batu dan Bumiaji dapat diidentifikasi dengan metode PCR RFLP dan teridentifikasi adanya alel D dan alel d. Perhitungan frekuensi alel memperlihatkan frekuensi alel D sebesar 67 % dan alel d sebesar 33 %. Mutasi ini tidak dikategorikan mutasi BLAD, melainkan polimorfisme gen.
English Abstract
Genetic disorders are disorders in livestock that are caused by mutations in genetic material. Defective alleles can be specific to certain breeds such as Bovine Leukocyte Adhesion Deficiency (BLAD) disease attacking the Holstein breed. Mutations in the CD18 gene can indicate the presence of BLAD in Friesian Holstein dairy cattle and their breeds. The purpose of this study was to determine whether the RFLP PCR method could identify abnormalities in the CD18 gene in the Cross Breed Friesian Holstein calf and know how to calculate the frequency of the CD18 subunit allele that caused BLAD. This study used 14 samples obtained from the population of Friesian Holstein Dairy Cattle calves in Batu and Bumiaji Districts. DNA amplification was carried out by the Polymerase Chain Reaction method using 5´-TCA ACG TGA CCT TCC GGA GG-3´ forward primer and 5´- CCC AGA TTC TTG ACG TTG AC-3´ reverse primer. Detection of the CD18 gene mutation was carried out through the RFLP (Restriction Fragment Length Polymorphism) technique using the XbaI restriction enzyme. In the 2% agarose electrophoresis results samples number 1-3 and 9-14 that have been in RFLP showed three pieces of the band which means the samples are heterozygous with Dd genotype. While sample number 4-8 shows two pieces of band, which means that the sample is homozygous dominant with DD genotype, and there is no recessive homozygous band with genotype dd. CD18 gene mutations in the population of Cross Breed Friesian Holstein calf in Batu and Bumiaji Districts can be identified by the PCR RFLP method and identified the allele D and allele d. The results of the allele frequency calculation show the frequency of allele D is 67% and allele d is 33%. This mutation is not categorized as BLAD mutation, but gene polymorphism.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2020/84/052003864 |
| Uncontrolled Keywords: | CD18, PCR RFLP, XbaI, Frekuensi Alel, CD18, RFLP PCR, XbaI, frequency of allel |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.2 Cattle and related animals > 636.21 Cattle for specific purposes |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 10 Aug 2020 06:34 |
| Last Modified: | 11 Apr 2023 02:54 |
| URI: | http://repository.ub.ac.id/id/eprint/181333 |
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Text (DALAM MASA EMBARGO)
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