Siska, Ade (2018) Pengaruh Escalating Dose Immunotherapy (EDI) Dengan Self Antigen dsDNA Terhadap Jumlah Koloni Salmonella Typhimurium Pada Hati Mencit Balb/C Model Lupus. Sarjana thesis, Universitas Brawijaya.
Abstract
Lupus Eritematosus Sistemik (LES) adalah penyakit autoimun sistemik kronis yang ditandai dengan respons imun hiperaktif dan produksi autoantibodi abnormal. Terapi imunosupresan dan steroid belum menunjukkan hasil yang memuaskan, selain itu dapat meningkatkan kerentanan infeksi. Terapi lain yang dikembangkan adalah escalating dose immunotherapy (EDI) dsDNA. Penelitian ini bertujuan untuk mengetahui pengaruh EDI dsDNA terhadap jumlah koloni Salmonella Typhimurium pada hati mencit Balb/C model lupus. Penelitian ini menggunakan 24 mencit Balb/C betina yang dibagi menjadi tiga kelompok; kelompok kontrol negatif, kelompok kontrol positif dan kelompok perlakuan. Kelompok kontrol positif dan perlakuan diinjeksi 0,5 mL pristan intraperitoneal. Setelah 12 minggu paska injeksi, kadar serologis dan manifestasi klinis dievaluasi. Kelompok perlakuan kemudian diberi EDI dsDNA yang dikomplekskan dengan polyethylenimine (PEI), secara bertahap (dosis 0,05μg, 0,5μg, 5μg) interval 1 minggu. Lalu ketiga kelompok disonde bakteri Salmonella Typhimurium 108 CFU/mL. Mencit dibedah dan dikultur hatinya di media BSA serta dihitung koloni bakterinya. Hasil analisis didapatkan EDI dengan dsDNA secara signifikan meningkatkan jumlah koloni bakteri dibandingkan kontrol positif (p=0,021) dan kontrol negatif (p=0,014). Pemberian escalating dose immunotherapy (EDI) dengan dsDNA dapat meningkatkan jumlah koloni Salmonella Typhimurium pada hati mencit model lupus.
English Abstract
Systemic lupus erythematosus (LES) is a chronic systemic autoimmune disease characterized by a hyperactive immune response and abnormal autoantibody production. Immunosuppressants and steroids therapy have not shown satisfactory results, furthermore it increases susceptibility to infections. Another developing therapy is escalating dose immunotherapy (EDI) dsDNA. This study aims to study the effect of EDI dsDNA on the number of Salmonella Typhimurium colonies in liver of Balb/C mice model lupus. This study used 24 female Balb/C mice divided into three groups; negative control group, positive control group, and treatment group. Positive control and treatment groups were injected 0.5 mL pristane intraperitoneally. After 12 weeks post injection, serological levels and clinical manifestations are evaluated. The treatment group was then given EDI dsDNA complexed with polyethylenimine (PEI), gradually (doses of 0.05μg, 0.5μg, 5μg) interval of 1 week. Then, all groups were given 108 CFU/mL Salmonella Typhimurium. Mice were dissected and cultured on BSA media and bacterial colonies were counted. The results showed EDI dsDNA significantly increased the number of bacterial colonies compared to positive control group (p = 0.021) and negative control group (p = 0.014). Escalating dose immunotherapy (EDI) dsDNA can increase the number of Salmonella Typhimurium colonies in the liver of mice model lupus.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/603/052001458 |
| Uncontrolled Keywords: | Lupus Eritematosus Sistemik, dsDNA, escalating dose immunotherapy, Salmonella Typhimurium-Systemic Lupus Erythematosus, dsDNA, escalating dose immunotherapy, Salmonella Typhimurium |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.7 Diseases of musculoskeletal system > 616.77 Diseases of connectives tissues > 616.772 Systemic lupus erythematosus |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 10 Aug 2020 06:56 |
| Last Modified: | 22 Oct 2021 03:30 |
| URI: | http://repository.ub.ac.id/id/eprint/180378 |
Preview |
Text
Ade Siska.pdf Download (7MB) | Preview |
Actions (login required)
 |
View Item |