Wardhani, Ananti Wulan (2019) Eksplorasi Bakteri Asam Laktat Endofit yang Berpotensi sebagai Probiotik dari Tanaman Obat. Sarjana thesis, Universitas Brawijaya.
Abstract
Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) endofit dari beberapa tanaman obat yang berpotensi sebagai probiotik, menguji viabilitas BAL probiotik terpilih selama fermentasi ekstrak tanaman obat dan mengidentifikasi spesies BAL terpilih yang memiliki potensi sebagai agen probiotik dan fermentasi ekstrak tanaman obat. Tujuh tanaman obat yang digunakan antara lain rimpang kunyit (Curcuma longa), rimpang temulawak (Curcuma xanthorrihza Roxb), bawang putih (Allium sativum), daun meniran (Phyllanthus urinaria), daun sirsak (Annona muricata L.), daun dan batang kelor (Moringa oleivera), dan buah mengkudu (Morinda citrifolia). Bakteri asam laktat diisolasi dengan menggunakan metode dilusi bertingkat dalam garam fisiologis (NaCl 0,85%) dan platting pada media MRS dan M17 agar yang mengandung 1% CaCO3. Isolat yang diperoleh dikarakterisasi lebih lanjut meliputi pewarnaan Gram dan uji katalase. Karakterisasi probiotik dilakukan dengan menguji ketahanan isolat terhadap kondisi asam (pH 2 dan 3), garam empedu (0,3% dan 2%) dan aktivitas antimikroba. Viabilitas kandidat probiotik terpilih juga diuji selama proses fermentasi ekstrak tanaman obat. Identifikasi isolat terpilih tersebut dilakukan berdasarkan sekuen 16S rDNA. Sebanyak 14 isolat berhasil diisolasi dari tujuh tanaman obat. Dua diantaranya yaitu isolat BP102 dari bawang putih dan KN101 dari rimpang kunyit memiliki karakteristik probiotik yang paling baik dan berpotensi sebagai kultur starter untuk fermentasi ekstrak tanaman obat. Isolat BP102 memiliki similaritas 99,99% dengan Lactobacillus plantarum dan isolat KN101 memiliki similaritas 99,99% dengan Enterococcus faecium.
English Abstract
This research aimed to obtain endophytic lactic acid bacteria (LAB) isolates from medicinal plants that have potency as probiotic, to examine the viability of the selected LAB with probiotic potency during fermentation of medicinal plant extracts and to identify the selected LAB isolates as both probiotic and fermenter of medicinal plant extract agents. Seven medicinal plants used namely turmeric, (Curcuma longa), java turmeric (Curcuma xanthorrihza Roxb), garlic bulbs (Allium sativum), chamber bitter leaves (Phyllanthus urinaria), soursop leaves (Annona muricata L.), leaves and stem of moringa (Moringa oleivera), and cheese fruit (Morinda citrifolia). Lactic acid bacteria were isolated using serial dilution in saline solution (0,85% NaCl) and then plated on MRS and M17 agar supplemented with 1% CaCO3. The isolates obtained were further characterized such as Gram staining and catalase test. The LAB isolates were examined in vitro for probiotic properties such as resistant to low pH, bile salt and antimicrobial activity. The viability of selected isolates during fermentation of medicinal plant extract was also examined. The identification of selected LAB was conducted based on 16S rDNA sequences. Fourteen isolates were isolated from seven medicinal plants. Among two of them were BP102 isolated from garlic bulbs and KN101 isolated from turmeric had the best probiotic characteristics and potential starter cultures for fermenting medicinal plant extract. Isolate BP102 showed 99,99% similarity to Lactobacillus plantarum and isolate KN101 presented 99,99% similarity with respect to Enterococcus faecium.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2019/386/052001541 |
| Uncontrolled Keywords: | bakteri asam laktat, endofit, tanaman obat, probiotik, endophyte, lactic acid bacteria, medicinal plants, probiotic |
| Subjects: | 500 Natural sciences and mathematics > 582 Plants noted for specific vegetative characteristics and flowers > 582.1 Herbaceous and woody plants, plants noted for their flowers > 582.12 Herbaceous plants |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 10 Aug 2020 07:37 |
| Last Modified: | 10 Aug 2020 07:37 |
| URI: | http://repository.ub.ac.id/id/eprint/179943 |
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