Fluorosensi Kalsium pada Sel Neuronal SH-SY5Y yang Dipapar Pilocarpine: Model Epilepsi In-Vitro.

Waafi, Afiyfah Kiysa (2019) Fluorosensi Kalsium pada Sel Neuronal SH-SY5Y yang Dipapar Pilocarpine: Model Epilepsi In-Vitro. Sarjana thesis, Universitas Brawijaya.

Abstract

Epilepsi adalah salah satu penyakit neurologis yang memiliki angka prevalensi tinggi dan menyebabkan disabilitas serta mortalitas. Patogenesis epilepsi yang masih belum jelas menuntut pemahaman yang lebih besar, sehingga diharapkan dapat mengembangkan terapi epilepsi baru yang dapat mencegah proses epileptogenik atau memodifikasi progresifitas epilepsi. Model penelitian epilepsi saat ini yang menggunakan hewan coba memerlukan model binatang yang kronik dan rumit serta membutuhkan banyak waktu. Sedangkan penelitian epilepsi secara in vitro saat ini menggunakan metode pengukuran patch clamp dan microelectrode array yang cukup sulit secara teknis dan harganya mahal. Sel SH-SY5Y berasal dari sel neuroblastoma manusia yang dapat berdiferensiasi menjadi neuron yang memiliki fitur morfologi, neurokimia, dan elektrofisiologis yang sesuai. Pilocarpin yang digunakan sebagai agen prokonvulsan adalah suatu agonis muskarinik yang dapat menyebabkan suatu kondisi yang disebut paroxysmal depolirizing shift (PDS) yang ditandai dengan peningkatan kalsium. PDS akan mengganggu keseimbangan reseptor eksitatori dan inhibitori yang akan menyebabkan hipereksitabilitas. Penelitian ini dilakukan secara in-vitro dengan Randomized Posttest Only Controlled Group Design dengan tujuan untuk membuktikan kultur sel SH-SY5Y yang dipapar dengan pilocarpin dapat menjadi model epilepsi in-vitro. Pada penelitian ini sel SH-SY5Y yang dipapar pilocarpin dosis 0 μM,1 μM, 5 μM, 10 μM, dan 15 μM akan diukur fluorosensi kalsiumnya menggunakan mikroskop konfokal. Hasil uji komparasi menggunakan One Way Anove menunjukkan hasil yang signifikan (p<0.05) pada perbandingan antar kelompok pada detik 13, 26, 39, 52, dan 65. Hasil penelitian juga menunjukkan pada dosis 5 uM menunjukkan dosis paling optimal dengan rata-rata fluorosensi kalsium yang paling tinggi.

English Abstract

Epilepsy is a neurological disease that has a high prevalence rate and can cause disability and mortality. The pathogenesis of epilepsy is still unclear, therefore requires a better understanding of it, so it is expected to develop new epilepsy therapy that can prevent epileptogenic processes or modify epileptic progression. The current epilepsy research model uses experimental animals that requires a chronic and complex animal model and a lot of time. Whereas in vitro epilepsy research is currently using patch clamp measurement methods and microelectrode arrays that are quite technically difficult and expensive. SH-SY5Y cells originate from human neuroblastoma cells which can be differentiated into neurons that have appropriate morphological, neurochemical and electrophysiological features. Pilocarpine as a proconvulsant agent is a muscarinic agonist that can cause an event called paroxysmal depolarizing shift (PDS). PDS then cause imbalance of excitatory and inhibitory receptors and will result in hiperexcitability. This study uses in-vitro method with Randomized Posttest Only Controlled Groups aimed to prove that SH-SY5Y cell culture induced by pilocarpin can be in vitro epileptic model. In this study the increase in calcium fluoroscence in SH-SY5Y cells after exposure to pilocarpin in dose 0 μM,1 μM, 5 μM, 10 μM, dan 15 μM will be measured using confocal microscope. Comparation study with One Way Anova showed that the comparation of all groups in the 13, 26, 39, 52, and 65 seconds are significant (p<0.05). And from this study we can conclude that dosage 5 is the most optimal dose with the highest calcium fluorescence average.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FK/2019/487/052001298
Uncontrolled Keywords: Epilepsi, Fluorosensi Kalsium, Pilocarpin, Sel SH-SY5Y-Calcium Fluoroscence, Epilepsy, Pilocarpine, SH-SY5Y Cell
Subjects: 600 Technology (Applied sciences) > 616 Diseases > 616.8 Diseases of nervous system and mental disorders > 616.89 Mental disorders > 616.891 Therapy
Divisions: Fakultas Kedokteran > Pendidikan Dokter
Depositing User: soegeng sugeng
Date Deposited: 18 Aug 2020 03:13
Last Modified: 22 Oct 2021 06:47
URI: http://repository.ub.ac.id/id/eprint/179876
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