Camielle, Centaura Naila Alfin (2019) Pengaruh Pemberianvitamin D3 Terhadap Ekspresi IL-10 Dan IL-12 Pada Sel Epitel Kolon Mencit Modelinflammatory Bowel Disease. Sarjana thesis, Universitas Brawijaya.
Abstract
Inflammatory Bowel Disease (IBD) pada anak-anak menyebabkan komplikasi meliputi gagal tumbuh dan pubertas yang terlambat, 60% mengalami hipovitaminosis D. Patogenesis IBD melibatkan peran Interleukin 10 (IL-10) dan IL-12 dalam meregulasi proses homeostasis system imun dan epitel usus. Vitamin D dengan bentuk aktif vitamin D3, memiliki sifat anti inflamasi, namun peran suplementasi vitamin D3 belum diketahui. Tujuan penelitian ini untuk mengetahui peran vitamin D3 terhadap ekspresi IL-10 dan IL-12 pada sel epitel kolon mencit model IBD. Penelitian ini merupakan eksperimental randomized control trial, 21 sampel ekor mencit, terbagi dalam 3 kelompok perlakuan, dilakukan di laboratorium farmakologi, biokimia-biomedik, patologi anatomi Fakultas Kedokteran Universitas Brawijaya Malang, Januari-Maret 2018. Suplementasi vitamin D3 dengan dosis 0.2 μg/25 gram/hari diberikan pada sampel setelah diinduksi dekstran 3% melalui sonde selama 7 hari. Untuk menentukan inflamasi digunakan skor DAI (Daily Activity Index) untuk tingkat inflamasi makroskopi,dan skor MCHI (The Mouse Collitis Histology Index) untuk tingkat inflamasi secara histopatologi. Parameter yang digunakan pada penelitian ini adalah ekspresi IL-10 dan IL-12 dengan menggunakan metode imunohistokimia, hasil positif dengan adanya perubahan warna inti sel kecoklatan pada epitel kolon mencit IBD. Data penelitian dilakukan uji Anova, Mann Whittney, dan ujibedaKruskal-Wallis, analisa menggunakan SPSS-23. Hasil Skor DAI di semua kelompok tampak pada penurunan berat badan, sedangkan skor MCHI tertinggi tampak pada kontrol positif. Terdapat perbedaan signifikan jumlah IL-10 antar kelompok control negative dengan control positif (p=0.038), control negative dengan perlakuan (p=0.001), dan control positif dengan perlakuan (p=0.017).UjiKruskal-Wallis antar kelompok, berbeda signifikan, dimana vitamin D3 meningkatkan jumlah IL-10 (p=0.001).Terdapat perbedaan signifikan jumlah IL-12 antar kelompok (p=0.000; p=0.000; p=0.000).Uji ANOVA antar kelompok berbeda signifikan, dimana vitamin D3 menurunkan jumlah IL-12 (p=0.000). Kesimpulandari penelitian ini pemberian vitamin D3 dapat meningkatkan ekspresi IL-10 dan menurunkan ekspresi IL-12 pada sel epitel kolon model mencit IBD.
English Abstract
Inflammatory Bowel Disease (IBD) in children can cause failed to grow and puberty delay, which is 60% have hypo-vitamin D. The pathogenesis of IBD involving interleukin 10 (IL-10) and IL-12 homeostatic regulation of immune system and intestine epithelial. Vitamin D3 (Vitamin D active form), has anti-inflammation properties, but the role of vitamin D3 supplementation still unknown. Aim this study to determine the role of vitamin D3 in IL-10 and IL-12 expression in colon epithelial cell of IBD rat model. This is a randomized control trial experiment with 21 samples, define to 3 groups, at pharmacology, biomedical, anatomic pathology laboratory Medical Faculty, University of Brawijaya, January -March 2018. Vitamin D3 supplementation at a dose 0.2 μg/25 gram/day was given to the sample after dextran 3% induced for 7 days. To determine inflammation, DAI (Daily Activity Index) and MCHI (The Mouse Colitis Histology Index) score for inflammation level. The parameters used in this study were the expression of IL-10 and IL-12 using the immunohistochemical method, a positive result with a change in the color of the brownish cell nucleus in the IBD mice colon epithelial. Statistical analysis were done by ANOVA, Mann-Whittney, and Kruskal Wallis utilizing SPSS-23. ResultDAI score found at body weight decreasing in all group, the highest MCHI score found at positive control group. There were significant IL-10 level between negative control and positive control group (p=0.038), negative control and experiment group (p=0.001), positive control and experiment group (p=0.017). Kruskal-Wallis between groups were significantly difference, vitamin D3 increase IL-10 level (p=0.001). There were significantly difference in IL-12 level between groups (p=0.000; p=0.000; p=0.000). Anova test were significantly difference between groups, vitamin D3 decrease IL-12 level (p=0.000). Conclusionthis study isVitamin D3 supplementation increase IL-10 but decrease IL-12 expression in colon epithelial cell of IBD rat model.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | TES/616.344/CAM/p/2019/042001968 |
| Uncontrolled Keywords: | IMFLAMMATORY BOWEL DISEASES |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.3 Diseases of digestive system > 616.34 Diseases of Intestine > 616.344 Enteritis / Inflammatory bowel diseases |
| Divisions: | S2/S3 > Magister Ilmu Biomedis, Fakultas Kedokteran |
| Depositing User: | Endang Susworini |
| Date Deposited: | 04 Mar 2020 01:54 |
| Last Modified: | 04 Mar 2020 01:54 |
| URI: | http://repository.ub.ac.id/id/eprint/179866 |
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