Hayu, Kumala Kurnia (2019) Pengaruh Ekstrak Kulit Kacang Tanah (Arachis hypogaea L.) terhadap Jumlah Osteoblas pada Tulang Tulang Alveolar Tikus Wistar (Rattus norvegicus) yang diinduksi Bakteri Aggregatibacter actinomycetemcomitans. Sarjana thesis, Universitas Brawijaya.
Abstract
Periodontitis adalah penyakit inflamasi pada jaringan periodontal yang disebabkan oleh beberapa macam bakteri, salah satunya bakteri Aggregatibacter actinomycetemcomitans (Aa). Periodontitis dapat menyebabkan kerusakan ligamen periodontal dan tulang. Kulit kacang tanah (Arachis hypogaea L.) memiliki kandungan flavonoid salah satunya luteolin yang dapat menurunkan produksi TNF-α, IL-1, IL-6, dan RANKL sehingga dapat meningkatkan jumlah osteoblas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kulit kacang tanah (Arachis hypogaea L.) terhadap jumlah osteoblas pada tulang alveolar tikus wistar yang diinduksi bakteri Aa. Penelitian ini bersifat eksperimental murni (true experimental) dengan rancangan randomized post test only control group design. 30 tikus strain wistar dibagi menjadi 5 kelompok. Kelompok kontrol negatif (K-) tikus tidak diinduksi bakteri Aa dan tidak diberi ekstrak kulit kacang tanah. Kelompok kontrol positif (K+) tikus diinduksi bakteri Aa dan tidak diberi ekstrak kulit kacang tanah. Kelompok perlakuan P1, P2, P3 tikus diinduksi bakteri Aa dan diberi ekstrak kulit kacang tanah dengan dosis masing-masing 50 mg/kgBB, 100 mg/kgBB, 200 mg/kgBB. Pada hari ke-50 tikus dikorbankan. Jaringan mandibula tikus diambil untuk kemudian dibuat preparat histologi dengan pengecatan HE. Hasil dari uji post-hoc menunjukkan terdapat perbedaan yang signifikan antara kelompok K (-) dan K (+), K (-) dan P1, K (-) dan P3, K (+) dan P2, K (+) dan P3, P1 dan P2, P1 dan P3, P2 dan P3, namun tidak diperoleh perbedaan yang signifikan antara kelompok K (-) dan P2, serta K (+) dan P1. Hasil uji korelasi pearson diperoleh nilai signifikansi sebesar 0,873 (positif). Kesimpulan penelitian ini adalah pemberian ekstrak kulit kacang tanah dapat meningkatkan jumlah osteoblas pada tulang alveolar tikus wistar yang diinduksi bakteri Aa.
English Abstract
Periodontitis is an inflammatory disease of the periodontal tissue caused by several types of bacteria, one of which is Aggregatibacter actinomycetemcomitans (Aa). Periodontitis can cause periodontal ligament and bone damage. Peanut skin (Arachis hypogaea L.) has flavonoid content, one of which is luteolin that can reduce the production of TNF-α, IL-1, IL-6, and RANKL expression so that it can increase the number of osteoblasts. This study aims to determine the effect of peanut skin extract (Arachis hypogaea L.) towards the number of osteoblasts on alveolar bone of wistar rats induced by Aa.This research is true experimental with a randomized post-test only control group design. 30 wistar strain rats were divided into 5 groups. The negative control group (K-) rats were not induced by Aa and were not given peanut shell extract. Positive control group (K +) rats were induced by Aa and were not given peanut shell extract. The treatment group P1, P2, P3 rats were induced by Aa and were given peanut shell extract with a dose of 50 mg/kgBW, 100 mg/kgBW, 200 mg/kgBW.On the 50th day the rats were sacrificed. The mandibular tissue of rat was taken and then histological preparations were made using HE staining. The results of post-hoc test indicate that there are significant differences between groups K (-) and K (+), K (-) and P1, K (-) and P3, K (+) and P2, K (+) and P3, P1 and P2, P1 and P3, P2 and P3, but there were no significant differences between groups K (-) and P2, as well as K (+) and P1. Pearson correlation test results showed a significance value of 0.873 (positive). The conclusion of this study is the administration of peanut skin extract can increase the number of osteoblasts on the alveolar bone of Aa-induced wistar rats.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2019/99/052001853 |
| Uncontrolled Keywords: | Aggregatibacter actinomycetemcomitans (Aa), kulit kacang tanah, osteoblas, tulang alveolar-Aggregatibacter actinomycetemcomitans, alveolar bone, osteoblasts, peanut skin |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry > 617.66 Extraction / Teeth--Extraction |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 19 Jul 2020 07:04 |
| Last Modified: | 23 Oct 2021 03:07 |
| URI: | http://repository.ub.ac.id/id/eprint/179146 |
Preview |
Text
Kumala Kurnia Hayu.pdf Download (4MB) | Preview |
Preview |
Text
MAJALAH_KUMALA KURNIA HAYU_15507040111039.pdf Download (3MB) | Preview |
Actions (login required)
 |
View Item |