Taufik, Muhamad (2019) Efektivitas ekstrak daun seledri (Apium graveolens L.) sebagai penghambat pertumbuhan bakteri Porphyromonas gingivalis secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Porphyromonas gingivalis merupakan bakteri melanogenik, non sakarolitik, dan bagian dari koloni bakteri black-pigmented Gram-negative anaerobs. Bakteri ini merupakan salah satu agen penyebab periodontitis yang sering ditemukan pada plak dan subgingiva. Berbagai pengendalian bakteri telah dilakukan seperti terapi antimikroba. Terapi antimikroba dapat menggunakan obat kumur seperti chlorhexidine gluconate. Penggunaan chlorehexidine gluconate dalam jangka panjang tidak dianjurkan karena menimbulkan berbagai efek samping. Penelitian ini bertujuan untuk memberi solusi alternatif dengan memanfaatkan bahan herbal seperti ekstrak daun seledri yang mengandung flavonoid, alkaloid, saponin, dan tannin yang merupakan senyawa antibakteri. Jenis penelitian ini ialah eksperimental laboratories dengan metode dilusi agar untuk menentukan Konsentrasi Hambat Minimum (KHM). Perlakuan dibagi menjadi 9 kelompok yaitu ekstrak daun seledri konsentrasi 1,25%, 1%, 0,75%, 0,5%, 0,25%, control negatif, dan control positif. Hasil penelitian menunjukkan terdapat perbedaan signifikan pada setiap kelompok perlakuan, kecuali pada 0,5% terhadap 1%, 1% terhadap 1,25% dan control positif yang tidak berbeda signifikan. Penelitian ini dapat disimpulkan bahwa ekstrak daun seledri efektif dalam menghambat pertumbuhan bakteri Porphyromonas gingivalis secara in vitro dengan konsentrasi terendah dari ekstrak daun seledri yang memiliki daya antibakteri adalah konsentrasi 1,25%.
English Abstract
Porphyromonasgingivalis is a melanogenic, non-saccholytic bacterium, and part of the Gram-negative anaerobs black-pigmented bacterial colony. It is one of the causative agents of periodontitis which is often found in plaques and subgingiva. There are some bacterial controls such as antimicrobial therapy. For example is using a moutwash such as chlorhexidine gluconate. The use of chlorhexidine gluconate in the long term is not recommended because it causes some side effects. This study aims to provide alternative solutions by utilizing herbal ingredients such as celery leaf extract that contains some antibacterial compound that is flavonoids, alkaloids, saponins and tannins. This study is a laboratory experiment with aagardilution method to determine the Minimum Inhibitory Concentration (MIC). The treatments were divided into 9 groups that are some celery leaf extract with some concentration (1,25%, 1%, 0,75%, 0,5%, 0,25%, negative controls, and positive controls). The result showed the there were significant differences in each treatment group, except at 0,5% against 1%, then 1% against 1.25% and positive controls that were mot significantly different. So that it can be concluded that the celery leaf extract was effective to inhibit the growth of Porphyromonasginigivalis bacteria by in vitro with the lowest concentration of celery leaf extract which had antibacterial power was a concentration of 1.25%.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKG/2019/120/052001882 |
| Uncontrolled Keywords: | daya antibakteri, flavonoid, alkaloid, saponin, tannin, ekstrak daun seledri, dilusi agar, Porphyromonas gingivalis-antibacterial power, flavonoids, alkaloids, saponins, tannins, celery leaf extract, agar dilutions, Porphyromonasgingivalis |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.6 Dentistry > 617.66 Extraction / Teeth--Extraction |
| Divisions: | Fakultas Kedokteran Gigi > Kedokteran Gigi |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 10 Aug 2020 07:50 |
| Last Modified: | 23 Oct 2021 02:35 |
| URI: | http://repository.ub.ac.id/id/eprint/179053 |
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