Dliyauddin, Moh (2019) Aktivitas Proteolitik Dan Kitinolitik Bakteri Indigeneous Limbah Cangkang Rajungan (Portunus Pelagicus) Asal Kabupaten Rembang Dan Pati. Magister thesis, Universitas Brawijaya.
Abstract
Produksi olahan perikanan rajungan untuk ekspor di Indonesia semakin meningkat. Total produksi rajungan untuk ekspor pada tahun 2017 diperkirakan mencapai 36.298 ton. Meningkatnya total produksi rajungan diikuti semakin banyaknya limbah dari hasil pengolahan rajungan yang sangat berpotensi mencemari lingkungan. Kandungan protein dan kitin yang cukup tinggi pada cangkang rajungan dapat dimanfaatkan untuk produksi turunan dari kitin dengan memanfaatkan bakteri penghasil enzim protease dan kitinase yang diharapkan mampu mengatasi permasalahan limbah cangkang rajungan sekaligus meningkatkan nilai jualnya. Tujuan penelitian ini adalah untuk mengisolasi, menguji potensi dan mengidentifikasi bakteri indigenous potensial dari limbah cangkang rajungan untuk memproduksi enzim ekstraselular protease dan kitinase. Bakteri proteolitik dan kitinolitik diisolasi dari sampel tanah tempat pembuangan limbah cangkang rajungan di Kecamatan Sluke Kabupaten Rembang dan Kecamatan Batangan Kabupaten Pati, Jawa Tengah dengan metode dilusi bertingkat. Bakteri proteolitik dan kitinolitik diisolasi pada media yang berbeda yaitu skim milk agar dan colloidal chitin agar secara pour plate, selanjutnya diamati kelimpahan sel dan morfologi koloni isolat bakteri yang diperoleh. Parameter lingkungan yang diukur seperti C-organik, bahan organik, kadar air, pH dan suhu. Bakteri proteolitik dan kitinolitik secara berurutan diseleksi dengan menguji aktivitas enzim yang dihasilkan secara kuantitatif pada media yang mengandung 1 % kasein dan 1 % kitin. Bakteri dengan aktivitas protease dan kitinase tertinggi diuji patogenitasnya pada media agar darah. Bakteri proteolitik dan kitinolitik potensial dioptimasi berdasarkan waktu inkubasi pada media kitin cair 0,5 % selama 48 jam pada shaker inkubator 120 rpm untuk diuji aktivitas proteolitik dan kitinolitik. Bakteri terpilih diidentifikasi berdasarkan sekuen 16S rDNA. Kelimpahan bakteri proteolitik dan kitinolitik dari Kabupaten Pati lebih tinggi yaitu 4,40 x 104 CFU/g dan 11,13 x 103 CFU/g dibandingkan dua lokasi di Kabupaten Rembang (Kab. Rembang A dan Kab. Rembang B) yang didukung dengan kandungan C-organik, bahan organik dan kadar air yang lebih tinggi. Total isolat bakteri proteolitik dan kitinolitik yang ditemukan secara berurutan sebanyak 23 dan 25 isolat. Isolat AP9 dan AP5 sebagai bakteri proteolitik dengan aktivitas sebesar 25,56 Unit mL-1 enzim per menit dan 24,93 Unit mL-1 enzim per menit secara berurutan, sedangkan bakteri kitinolitik dipilih isolat BP14 dan CK20 dengan aktivitas sebesar 0,796 Unit mL-1 enzim per jam dan 0,718 Unit mL-1 enzim per jam secara berurutan. Isolat bakteri yang bersifat non pathogen yaitu Isolat AP5 dan CK20, sedangkan isolat BP14 dan AP9 terindikasi bersifat patogen. Isolat AP9 memiliki aktivitas protease tertinggi sebesar 32,03 Unit mL-1 enzim per menit dengan waktu inkubasi 44 jam, sedangkan isolat BP14 memiliki aktivitas kitinase tertinggi sebesar 0,813 Unit mL- 1 enzim per jam dengan waktu inkubasi 32 jam. Isolat bakteri terpilih teridentifikasi dalam kelompok Genus Bacillus berdasarkan sekuen 16S rDNA. Isolat AP9 teridentifikasi sebagai Bacillus subtilis, sedangkan isolat AP5 dan CK20 sebagai Bacillus licheniformis dan isolat BP14 sebagai Bacillus cereus.
English Abstract
Indonesian exports of fishery products of the blue swimming crab are increasing. The total production of blue swimming crab exports in 2017 is estimated at 36,298 tons. The increase in blue swimming crab export is followed by the increase of shell waste produced from the processing product which has the potential to contaminate the environment. The blue swimming crab shell contains high protein and chitin so it can be used for the production of chitin derivatives using proteolytic and chitinolytic bacteria. The aims of this study were to isolate, test the potential and identify potential indigenous bacteria from crab shell waste to produce extracellular proteases and chitinase enzymes. Proteolytic and chitinolytic bacteria were isolated from soil samples of blue swimming crab shell waste disposal in Sluke District, Rembang Regency and Batangan District Pati Regency, Central Java by the multilevel dilution method. Proteolytic and chitininolytic bacteria were isolated in different media, skim milk agar and colloidal chitin agar in a pour plate method, then the abundance of cells and morphology of bacterial isolates obtained. Environmental parameters such as C-organic, organic matter, moisture content, pH and temperature are measured. Proteolytic and chitinolytic bacteria were sequentially selected by testing the enzyme activity produced quantitatively on media containing 1% casein and 1% chitin. Bacteria with the highest protease and chitinase activity were tested for their pathogenicity on blood agar media. Potential proteolytic and chitinolytic bacteria are optimized based on the incubation time on 0.5% liquid chitin media for 48 hours on a 120 rpm incubator shaker to assayed for proteolytic and chitinolytic activity. The selected bacteria were identified based on the 16S rDNA sequence. The abundance of proteolytic (4.40 x 104 CFU/g) and chitinolytic (11.13 x 103 CFU/g) bacteria from Pati Regency was higher than two lications in Rembang Regency (Rembang A Regency and Rembang B Regency). Its supported by C-organic content, organic materials and high water content. A total isolates of proteolytic and chitinolytic bacteria were found 23 and 25 isolates, respectively. AP9 and AP5 isolates as a proteolytic bacteria have a different activity. The isolate AP9 has an activity of 25.56 mL-1 enzyme units per minute and isolate AP5 of 24.93 mL-1 enzyme units per minute. While chitinolytic bacteria were selected isolates BP14 and CK20 with an activity of 0.796 mL-1 enzyme units per hour and 0.718 mL-1 enzyme units per hour, respectively. Bacterial isolates that are non pathogenic are AP5 and CK20 isolates, while BP14 and AP9 isolates are indicated to be pathogenic. The AP9 isolates had the highest protease activity of 32.03 mL-1 enzyme units per minute with an incubation time of 44 hours, while BP14 isolates had the highest chitinase activity of 0.813 mL-1 enzyme units per hour with an incubation time of 32 hours. Selected bacterial isolates were identified in the Bacillus Genus group based on the 16S rDNA sequence. The AP9 isolates were identified as Bacillus subtilis, while AP5 and CK20 isolates as Bacillus licheniformis and BP14 isolates as Bacillus cereus.
Other obstract
-
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/379.3/DLI/a/2019/041911355 |
| Uncontrolled Keywords: | BACTERIA, PROTEOLYTIC ENZYMES, CHITINASE, PORTUNUS |
| Subjects: | 300 Social sciences > 379 Public policy issues in education > 379.3 Public policy issues in private education |
| Divisions: | S2/S3 > Magister Biologi, Fakultas MIPA |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 08 Jan 2020 02:49 |
| Last Modified: | 25 Oct 2021 03:48 |
| URI: | http://repository.ub.ac.id/id/eprint/177628 |
Preview |
Text
Moh Dliyauddin (2).pdf Download (1MB) | Preview |
Actions (login required)
 |
View Item |