Setyoningrum, Desy (2019) Studies On The Effect Of Rice Husk Silica Liquid On Immune Modulation And Diseases Resistance In Zebrafish (Danio Rerio). Magister thesis, Universitas Brawijaya.
Abstract
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English Abstract
Global aquaculture production has increased in recent years and it is predicted that aquaculture will provide the most reliable supply of seafood in the future. However, there are many controversial issues in aquaculture, one of them is that the overuse and misuse of antibiotics may lead to environmental pollution problems, a food safety hazard and also can affect the development of antibiotic-resistant bacteria. Rice husk is a by-product produced abundantly in rice production that it has low commercial value. The ash of rice husk mainly consists >90% silica. Silica is the biogenic compound that is considered necessary for various vital functions, although its content in the tissue of a living organism is very traced. This study is focused on the evaluation of application potential the potential of rice husk silica (RHS) as immune stimulator to against aquatic pathogens in aquaculture and its antibacterial activities. Assay of antibacterial activity, in vivo challenge test iv and quantitative RT-PCR to determine the expression level of immune-related genes, oxidative stress and apoptosis-related genes carried out in this study. In the present study, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assay results demonstrated that RHS bacteriostatic and bactericidal potency was lower than kanamycin, but RHS‟s antimicrobial activities against antibiotic-resistant pathogens V. alginolyticus, V. parahaemolyticus, V. vulnifius and Methicillin-resistant S. aureus (MRSA) was effective. The expression of all immune-related genes analysis such as IL-1β, IL-6, IL-15, TNF-α, TLR-4α, COX-2α, complement c3b and lysozyme in zebrafish body were significantly increased in the RHS group compared with the control group, the oxidative stress such as SOD1, Gpx1α, NOS2α, and Hsp 70, and apoptosis-related genes such as Bcl-2 and tp53 were observed on zebrafish that immersed with RHS were not significantly different compared with control. The bacterial challenge test results demonstrated that RHS liquid significantly enhanced the survival rate in zebrafish infected with S. iniae and A. hydrophila. In conclusion, our results suggested that RHS can be used as an antimicrobial agent and as an immunostimulant to protect bacterial-infected fish in aquaculture.
Other obstract
近年來全球水產養殖業的產量持續增加，預計未來將提供水產品穩定的產量。 然而，水產養殖業中存在許多爭議性問題，其中最主要的原因是過量使用抗生素，此舉可能導致環境污染問題、食品安全危害以及造成抗抗生素細菌的產生。稻殼是水稻生產中大量生產的副產品，其商業價值低。 稻殼灰主要由 90％以上的二氧化矽組成，而二氧化矽是生物化合物，被認為是各種生命功能所必需的，但是它在生物體中的含量卻是非常微量。本研究的重點為評估稻殼二氧化矽（RHS）作為免疫調節劑，對於水產養殖中病原菌的抗菌活性以及應用潛力。本研究測定RHS對於水產養殖中病原菌的抗菌活性、感染實驗以及使用qPCR分析免疫反應、氧化反應與細胞凋亡的相關基因表現。經由最小抑菌濃度（MIC）和最小殺菌濃度（MBC）的測定結果表明RHS的抑菌效果與殺菌效果低於Kanamicyn，但RHS對於抗抗生素病原菌V. alginolyticus、V. parahaemolyticus、V. Vulnifius以及Methicillin-resistant S. aureus (MRSA) 具有抑制的效果。斑馬魚浸泡結果顯示，相較於控制組，RHS組中的免疫基因IL-1β、IL-6、IL-15、TNF-α、TLR-4α、COX-2α、 Dr. Ir. Maftuch, M. Si. ii complement c3b以及溶菌酶均有顯著增加，而氧化基因SOD1、Gpx1α、NOS2α、Hsp70 以及細胞凋亡基因Bcl-2 、 tp53並沒有明顯的差異。感染試驗的結果表明，RHS液體可以提高受到S. iniae與A. hydrophila感染的斑馬魚存活率。本研究結果表明，RHS可作為抗菌劑以及免疫調節劑，避免水產養殖產業受病原菌的威脅。
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/639.3/SET/s/2019/041902958 |
| Uncontrolled Keywords: | ANTIBIOTICS IN AQUAACULTURE, ZEBRA DANIO, IMMUNITY |
| Subjects: | 600 Technology (Applied sciences) > 639 Hunting, fishing & conservation > 639.3 Culture of cold-blooded vertebrates |
| Divisions: | S2/S3 > Magister Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 06 Jan 2020 08:05 |
| Last Modified: | 21 Oct 2021 03:32 |
| URI: | http://repository.ub.ac.id/id/eprint/177581 |
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