Arifah, Annisa Novianti (2019) Pengaruh Pemberian Infusa Daun Beluntas (Pluchea Indica L)Terhadap Ekspresi Tnf-Α Dan Gambaran Histopatologi Ileum Pada Mencit (Mus Musculus) Model Diare Hasil Induksi Castor Oil(Oleum Ricini). Sarjana thesis, Universitas Brawijaya.
Abstract
Diare merupakan gangguan pencernaan yang ditandai dengan frekuensi defekasi yang melebihi frekuensi normal, konsistensi yang encer dan bersifat akut maupun kronis. Bahan induksi diare yang digunakan pada penelitian ini adalah minyak jarak (castor oil) yang bersifat iritan pada saluran pencernaan. Infusa daun beluntas dapat digunakan sebagai bahan pengobatan diare karena mengandung flavonoid dan tanin yang berguna dalam proses penyembuhan diare. Penelitian ini bertujuan untuk mengetahui pengaruh infusa daun beluntas (Pluchea indica L.) terhadap ekspresi Tumor Necrosis Factor Alpha (TNF-α) ileumdan gambaran histopatologi ileum pada mencit (Mus musculus) model diare hasil induksi castor oil. Hewan coba yang digunakan sebanyak 30 ekor mencit jantan dengan berat badan 20-25 gram berusia 40-60 hari yang dibagi menjadi 6 kelompok perlakuan yaitu kontrol negatif (tanpa perlakuan/mencit sehat), kontrol positif (diinduksi castor oil tanpa diterapi), kelompok terapi pembanding (diinduksi castor oil dan diterapi norit®), kelompok perlakuan 1,2,3 (diinduksi castor oil dan terapi infusa daun beluntas konsentrasi bervariasi 5, 10, 20%). Pengamatan ekspresi TNF-α dengan metode imunohistokimia dideskripsikan menggunakan software immunorasio yang dianalisa dengan uji One-Way Anovadan uji lanjutan Tukey dengan tingkat kepercayaan 95% (α= 0,05) dan memperoleh hasil uji yang menunjukkan bahwa mencit diare yang diterapi infusa daun beluntas mengalami penurunan ekspresi TNF-α. Pengamatan gambaran histopatologi ileum dengan pewarnaan Haematoxyllin-Eosin (HE) dianalisa secara deskriptif kualitatifberdasarkan kerusakan jaringan mukosa ileum. Hasil penelitian menunjukkan bahwa terapi infusa daun beluntas secara signifikan (p<0,05) mampu menurunkan ekspresi TNF-α dan memperbaiki histopatologi ileum dengan konsentrasi terbaik adalah 20%. Kesimpulan dari penelitian ini yaitu pemberian infusa daun beluntas (Pluchea indica L.)dengan konsentrasi 20% dapat digunakan sebagai alternatif terapi diare pada mencit (Mus musculus).
English Abstract
Diarrhea is a digestive disorder characterized by a frequency of defecation that exceeds the normal frequency, the consistency is dilute and is acute or chronic. Diarrhea induction material used in this study was castor oil which stimulates irritation in the digestive tract. Beluntas leaf infusion can be used as a treatment for diarrhea because it contains flavonoids and tannins which are useful in the healing process of diarrhea. This study was aimed to determine the effect of infusion of beluntas (Pluchea indica L.) leaves on expression of Tumor Necrosis Factor Alpha (TNF-α) and ileal histopathology in mice (Mus musculus) diarrhea model caused by castor oil induction. The animal used in this study was 30 male mice with a body weight of 20-25 grams aged 40-60 days, which were divided into 6 treatment groups namely negative control (without treatment / healthy mice), positive control (castor oil induced, without therapy), therapy comparison group (castor oil induced and treated with norit®), and treatment group 1, 2, 3 (castor oil induced and infusion therapy of beluntas leaves concentration varied 5, 10, 20%). Observation of TNF-α expression by immunohistochemistry method was described using immunoratio software that was analyzed by One-Way Anova test and Tukey follow-up test with a confidence level of 95% (α = 0.05) and obtained test results showed that diarrhea mice treated with infusion of beluntas leaves decreased TNF-α expression. Observation of ileal histopathology with Haematoxyllin-Eosin (HE) stain was analyzed descriptively and qualitatively based on ileal mucosal tissue damage, and the results of the study showed that therapy of beluntas leaf infusion was able to reduce damage to ileal mucosal tissue. The results of the study showed that the beluntas leaf infusion can reduce the expression of TNF-α significantly (p<0,05) and reduce the damage of ileal mucosal tissue with optimal dose of 20% beluntas leaf infusion.. The conclusion of this study is the dose of 20% beluntas leaf infusion can be used as an alternative therapy for diarrhea in mice(Mus musculus).
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/167/051909677 |
| Uncontrolled Keywords: | Diare, daunbeluntas, TNF-α, histopatologi ileum, Diarrhea, beluntas leaves, TNF-α, ileum histopathology |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine > 636.089 5 Veterinary medicine (Pharmacology and therapeutics) > 636.089 532 1 Veterinary medicine (Drugs derived from plants) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 16 Nov 2020 10:30 |
| Last Modified: | 21 Oct 2021 06:32 |
| URI: | http://repository.ub.ac.id/id/eprint/177030 |
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