Esti, Yumna (2019) Pengaruh Pemberian Air Rebusan Jamur Kuping Hitam (Auricularia Polytricha) Terhadap Kadar Malondialdehida Dan Histopatologi Hepar Pada Tikus Putih (Rattus Novergicus) Model Hiperkolesterolemia. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia adalah kondisi ketika kadar kolesterol meningkat di dalam tubuh, mengindikasikan akumulasi radikal bebas. Dalam kondisi ini uji MDA dapat digunakan untuk pengujian radikal bebas. Peningkatan radikal bebas ini menyebabkan antioksidan alami di dalam tubuh tidak mampu menangani, sehingga dibutuhkan antioksidan yang berasal dari luar, yang dapat ditemukan pada air rebusan jamur kuping hitam. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian air rebusan jamur kuping hitam terhadap kadar MDA dan histopatologi organ hepar pada tikus putih (Rattus novergicus) model hiperkolesterolemia. Penelitian ini bersifat eksperimental (RAL). Hewan model menggunakan tikus putih (Rattus novergicus) strain Wistar jantan, yang dibagi menjadi lima kelompok perlakuan, yaitu kelompok negatif, kelompok hiperkolesterol, dan kelompok terapi yang diberi perlakuan air rebusan dengan sediaan pemberian 1, 2, dan 3 mL. Data kadar MDA hepar dianalisa secara kuantitatif (oneway ANOVA) dan histopatologi hepar dianalisa secara deskriptif. Hasil rata-rata kadar MDA antar kelompok perlakuan antara lain : (-) 458,56±40,54 ; (+) 496,89±32,75 ; (P1) 458,28±38,20 ; (P2) 450,22±30,97 ; (P3) 457,73±31,35. Hasil analisis data menunjukkan bahwa tidak terdapat perbedaan yang signifikan antar kelompok perlakuan terhadap penurunan kadar MDA (P>0,05). Kesimpulan penelitian ini adalah pemberian terapi air rebusan jamur kuping hitam tidak dapat menurunkan kadar MDA, walaupun ada kecenderungan menurun. Demikian juga terdapat perbaikan histopatologi hepar pada tikus model hiperkolesterolemia.
English Abstract
Hypercholesterolemia is a condition when cholesterol levels increase in the body, indicating the accumulation of free radicals. In this condition the MDA test can be used for free radical testing. This increase in free radicals causes the endogenous antioxidants unable to handle, so that needed exogenous antioxidants, which can be found in decoction of black ear fungus. This study aims to determine the effect of giving decoction of black ear fungus to MDA levels and liver organ histopathology in white rat (Rattus novergicus) model of hypercholesterolemia. This research is experimental (CRD). Animal models used male white rats (Rattus novergicus), strain Wistar which were divided into five treatment groups, including the negative group, the hypercholesterol group, and the treatment group treated with boiled water with 1, 2 and 3 mL. Data on liver MDA levels were analyzed quantitatively (oneway ANOVA) and liver histopathology was analyzed descriptively. The average results of MDA levels between treatment groups include: (-) 458.56 ± 40.54; (+) 496.89 ± 32.75; (P1) 458.28 ± 38.20; (P2) 450.22 ± 30.97; (P3) 457.73 ± 31.35. The results of the data analysis showed that there were no significant differences between the treatment groups for the reduction in MDA levels (P> 0.05). The conclusion of this study is that the treatment of black mushroom decoction therapy cannot reduce MDA levels, although there is a downward trend. Likewise there is improvement in liver histopathology in hypercholesterolemic rats.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/174/051910890 |
| Uncontrolled Keywords: | hiperkoleterolemia, jamur kuping hitam, MDA, histopatologi, hepar, hypercholesterolemia, black ear fungus, MDA, histopathology, liver |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.085 Feeds and applied nutrition > 636.085 2 Applied nutrition |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:44 |
| Last Modified: | 21 Oct 2021 06:41 |
| URI: | http://repository.ub.ac.id/id/eprint/177010 |
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