Putri, Hana Mitsuki (2019) Analisis Mutasi Gen Pancreatic Duodenal Homeobox 1 (Pdx-1) Berdasarkan Pada Kadar Glukosa Darah Kucing Domestik (Felis Catus) Post Ovariohisterektomy (Oh) Dengan Metode Polymerase Chain Reaction (Pcr). Sarjana thesis, Universitas Brawijaya.
Abstract
Ovariohisterectomy pada kucing domestik memiliki beberapa dampak salah satunya adalah obesitas sedangkan obesitas erat kaitannya dengan penyakit diabetes. Gen PDX-1 merupakan salah satu gen glukoregulator yang mana diduga jika terjadi mutasi akan mengakibatkan resistensi insulin dan berdampak pada kadar glukosa darah. Penelitian ini bertujuan untuk mengetahui hubungan dari mutasi gen PDX-1 dengan kadar glukosa darah kucing domestik post OH. Metode yang digunakan pada penelitian ini adalah amplifikasi invitro PCR gen PDX-1 dengan primer HN_F ‘CCG AGC TGT CAA AGC TAG CG’ dan HN_R ‘GAA CCG GTA GAG GGT CTT GG’dan dilanjutkan dengan sekuensing terhadap produk PCR. Hasil penelitian menunjukkan bahwa ada perbedaan basa pada sekuen gen PDX-1 pada c.435G>A, c.927A>T, dan c.584A>G. Perbedaan basa pada sekuen gen PDX- 1 merupakan silent mutation , sehingga tidak mengubah sekuen asam amino yang dikode. Berdasarkan hasil tersebut mutasi gen PDX-1 tidak memiliki hubungan spesifik terhadap kadar glukosa darah pada kucing domestik OH.
English Abstract
Ovariohisterectomy in domestic cats has several effects, one of which is obesity while obesity is closely related to diabetes. PDX-1 gene is one of the glucoregulator genes which is suspected if mutation occurs which will lead to insulin resistance and have an impact on blood glucose levels. This study aimed to determine the relationship of PDX-1 gene polymorphism to post OH domestic cat blood glucose levels. The method used in this research is invitro PCR amplification of PDX-1 gene using primer HN_F ‘CCG AGC TGT CAA AGC TAG’ and HN_R ‘GAA CCG GTA GAG GGT CTT GG’ and continued with sequencing of PCR products. The result showed the difference bases of PDX-1 gene sequence isolated from Domestic cats ( Felis catus) are c.435G> A, c.927A> T, and c.584A> G, as a silent mutations that do not change the sequence of amino acids encoded.The PDX-1 gene mutation has no specific relationship to blood glucose levels on domestic cats OH
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/111/051909043 |
| Uncontrolled Keywords: | Kucing domestik, Ovariohisterectomy, Gen PDX-1, Mutasi gen, glukosa darah, Domestic cats, Ovaryohisterectomy, Obesity, PDX-1 gene, Gen Muatation, Blood glucose |
| Subjects: | 500 Natural sciences and mathematics > 599 Mammalia (Mammals) > 599.7 Carnivora |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 02 Nov 2020 13:45 |
| Last Modified: | 21 Oct 2021 04:08 |
| URI: | http://repository.ub.ac.id/id/eprint/176955 |
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