Nadliroh, Ulfa Luluk (2019) Efek Preventif Ekstrak Kulit Pisang Kepok (Musa Paradisiaca) Terhadap Kadar Malondialdehida (Mda) Dan Histopatologi Jejunum Pada Tikus Putih (Rattus Norvegicus) Model Hiperkolesterolemia. Sarjana thesis, Universitas Brawijaya.
Abstract
Hiperkolesterolemia adalalah suatu kondisi dimana kolesterol di dalam darah meningkat melebihi normal.Hiperkolesterolemia dapat disebabkan oleh faktor lingkungan dan genetik. Penelitian ini bersifat True Experimental menggunakan Rancangan Acak Lengkap (RAL). Penelitian ini dilakukan untuk melihat aktivitas preventif ekstrak kulit pisang kepok (Musa paradisiaca) dalam mencegah hiperkolesterolemia dengan mengukur kadar malondialdehida dan melihat gambaran histopatologi jejunum pada tikus putih (Rattus norvegicus) model hiperkolesterolemia. Hewan model menggunakan tikus putih (Rattus norvegicus) jantan Strain Wistar yang berumur 8-10 minggu, dengan berat badan sekitar 100-150g. Penelitian ini dibagi dalam 5 kelompok perlakuan yaitu kelompok negatif, kelompok positif, dan kelompok perlakuan 1, 2, dan 3. Ekstrak kulit pisang kepok (Musa paradisiaca) mengandung pectin, tannin, saponin, dan flavonoid yang dapat menurunkan kadar kolesterol di dalam tubuh. Ekstrak kulit pisang kepok (Musa paradisiaca) diberikan selama 14 hari dengan dosis 22.05mg/150gBB, 44.1mg/150gBB, dan 88.2mg/150gBB. Pemberian diet hiperkolesterolemia dengan komposisi asam kholat 0,1%, minyak babi 10%, dan kuning telur puyuh 5%, diberikan selama 14 hari dengan dosis 3.02g/150gBB dengan sonde lambung selama 21 hari. Analisis data dilakukan secara kuantitatif dan kualitatif. Data kuantitatif yang digunakan yaitu pemeriksaan kadar malondialdehida pada organ jejunum menggunakan uji ANOVA dengan taraf kepercayaan α = 0,05 dan dilanjutkan dengan uji Turkey (p < 0,01). Data kualitatif yang digunakan yaitu gambaran histopatologi jejunum yang akan dianalisis dan disajikan secara deskriptif. Hasil penelitian ini menunjukkan bahwa semua dosis pemberian ekstrak kulit pisang kepok (Musa paradisiaca) dapat mencegah peningkatan kadar malondialdehida dan mencegah erosi epitel, hipertrofi dan hiperplasia sel goblet pada gambaran histopatologi tunika mukosa jejunum. Kesimpulan bahwa dosis 88.2mg/150gBB merupakan dosis efektif untuk mencegah peningkatan kadar malondialdehida dan mencegah kerusakan histopatologi jejunum pada tikus model hiperkolesterolemia.
English Abstract
Hypercholesterolemia is a condition where cholesterol in the blood increases more than normal. Hypercholesterolemia can be caused by environmental and genetic factors. This research is True Experimental using a Completely Randomized Design (RAL). This study was conducted to look at the preventive activity of kepok banana peel extract (Musa paradisiaca) in preventing hypercholesterolemia by measuring the levels of malondialdehyde and seeing histopathology of jejunum in rats (Rattus norvegicus) model of hypercholesterolemia. Model animals use Wistar strain 8-10 weeks old male Rattus norvegicus, with a body weight of around 100-150g. The study was divided into 5 treatment groups namely negative group, positive group, and treatment group 1, 2, and 3. Kepok banana peel extract (Musa paradisiaca) contained pectin, tannin, saponins, and flavonoids which can reduce cholesterol levels in the body. Kepok banana peel extract was given for 14 days at a dose of 22.05 mg / 150gBB, 44.1mg / 150gBB, and 88.2mg / 150gBB. The diet of hypercholesterolemia with a composition of 0.1% kholat acid, 10% pork oil, and 5% quail egg yolk, was given for 14 days at a dose of 3.02g / 150gBB with a gastric sonde for 21 days. Data analysis was carried out quantitatively and qualitatively. Quantitative data used were examination of malondialdehyde levels in jejunal organs using the ANOVA test with a confidence level α = 0.05 and continued with the Turkey test (p <0.01). The were qualitative data used are histopathological images of the jejunal that will be analyzed and presented descriptively. The results of this study indicate that all doses administered kepok banana peel extract can prevent the increase in malondialdehyde levels and prevent epithelial erosion, goblet cell hypertrophy and hiperplacia in histopathological features of the jejunal mucosa. The conclusion that the dose of 88.2mg/150gBW is an effective dose to prevent an increase malondialdehide levels and prevent jejunal histopathology damage in rats model of hypercholesterolemia.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/122/051909052 |
| Uncontrolled Keywords: | Ekstrak Kulit Pisang Kepok, Hiperkolesterolemia, Histopatologi Jejunum, Malondialehida, Hypercholesterolemia, Jejunum Histopathology, Kepok Banana Peel Extract, Malondialehide . |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.321 Drugs derived from plants |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 06:35 |
| Last Modified: | 21 Oct 2021 04:18 |
| URI: | http://repository.ub.ac.id/id/eprint/176920 |
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