Rafrensca, Annisa Rizqi (2019) Terapi Vco (Virgin Coconut Oil) Hasil Pengasaman Jeruk Nipis Untuk Hewan Model Luka Insisi Nosokomial Berdasarkan Kadar Tnfdan Jumlah Sel Radang Pada Kulit Mencit. Sarjana thesis, Universitas Brawijaya.
Abstract
Virgin Coconut Oil (VCO) merupakan hasil pengolahan kelapa yang mengandung asam laurat dan berfungsi sebagai antibakteri, mempercepat proliferasi fibroblas dan antiinflamasi. VCO berpotensi sebagai alternatif penanganan infeksi luka nosokomial akibat Staphylococcus aureus. Penelitian ini bertujuan untuk mengetahui VCO sebagai terapi hewan model luka insisi nosokomial terhadap penurunan kadar TNF-α dan jumlah sel radang. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dan post control only. Penelitian menggunakan mencit (Mus musculus) BALB/c jantan berat 30g berumur 8 minggu. Mencit diinsisi dan dilakukan penjahitan menggunakan benang silk 4/0 yang dikontaminasi S. aureus. Hewan coba dibagi dalam kelompok kontrol negatif (dijahit dan tidak diinfeksi S. aureus )¸ kelompok kontrol positif (dijahit dan diinfeksi S. aureus), dan kelompok yang diinsisi, diinfeksi S. aureus dan diterapi VCO, terapi 1x sehari (P1), terapi 2x sehari (P2), dan terapi 3x sehari (P3). Pemberian VCO dilakukan selama 7 hari secara topikal. Kulit mencit diambil untuk histopatologi pewarnaan Hematoxyline-Eosin (HE) dan untuk uji flow cytometer mengetahui kadar TNF-α. Perhitungan sel radang dilakukan dari 5 lapang pandang tiap sampel yang di rata-rata. Data dianalisis menggunakan uji one way ANOVA dan uji lanjutan BNJ (Beda Nyata Jujur) dengan tingkat kepercayaan 95% (α = 0,05). Hasil menunjukkan kelompok P1 (terapi VCO 1x sehari) mampu menurunkan kadar relatif TNF-α (48,82%) dan jumlah sel radang (56,25%) pada kulit hewan model luka insisi nosokomial. Kesimpulan pada penelitian ini yaitu VCO dapat digunakan sebagai alternatif terapi luka insisi nosokomial.
English Abstract
Virgin Coconut Oil (VCO) is the result of processing coconut which contains lauric acid and functions as an antibacterial, accelerating the proliferation of fibroblasts and anti-inflammatory. VCO has the potential as an alternative treatment for nosocomial wound infections due to Staphylococcus aureus. This study aims to determine VCO as a therapy form animal model of nosocomial incision wounds to decrease TNF-α levels and the number of inflammatory cells. The design of study a Complete Randomized Design (RAL) and post control only. This study used experimental mice (Mus musculus) BALB/c male weighing 30g aged 8 weeks old. Mice were incised using 4/0 silk thread contaminated with S. aureus. Experimental animals were divided into negative control group (sutured and not infected by S. aureus), positive control group (sutured and infected by S. aureus), and the incised group, infected by S. aureus and treated by VCO, therapy once a day (P1), therapy 2x a day (P2), and therapy 3x a day (P3). Giving VCO is done for 7 days topically. Mice skin was taken for Hematoxyline-Eosin (HE) staining histopatology and for flow cytometer test to khow TNF-α levels. Inflammation cell calculations were performed and 5 visual fields per sample were averaged. Data were analyzed using one way ANOVA test and continued BNJ test (Different, Real, Honest) with a confidence level of 95% (α = 0.05). The results showed that P1 group (VCO therapy once a day) was able to reduce the relative levels of TNF-α (48.82%) and the number of inflammatory cells (56.25%) in Mus musculus’s skin with nosocomial incision wounds model. The conclusion of this study is that VCO can be used as an alternative therapy for nosocomial incision wounds.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/123/051909053 |
| Uncontrolled Keywords: | Virgin Coconut Oil, Nosokomial, Sel radang, TNF-α, Virgin Coconut Oil, Nosocomial, Inflammatory Cells, TNF-α |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry > 636.089 Veterinary medicine > 636.089 5 Veterinary medicine (Pharmacology and therapeutics) > 636.089 532 1 Veterinary medicine (Drugs derived from plants) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 16 Nov 2020 15:47 |
| Last Modified: | 21 Oct 2021 04:19 |
| URI: | http://repository.ub.ac.id/id/eprint/176919 |
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