Budiarto, Fajruli (2019) Diversitas Genetik Cabai Rawit Lokal Malang Berdasarkan Karakter Morfologi, Kandungan Capsaicin dan Profil Simple Sequence Repeat (SSR). Sarjana thesis, Universitas Brawijaya.
Abstract
Cabai rawit (Capsicum frutescens L.) merupakan salah satu tanaman hortikultura dari famili Solanaceae yang digemari masyarakat Indonesia. Cabai rawit terdiri dari beberapa varietas yang dapat ditemukan di berbagai daerah di Indonesia, tidak terkecuali di kota Malang. Tujuan dari penelitian ini adalah mendeskripsikan dan menganalisis diversitas genetik tanaman cabai rawit G1M4, G4, G17, G18, dan CH berdasarkan karakter morfologi, kandungan capsaicin, dan profil Simple Sequence Repeat (SSR). Deskripsi karakter morfologi mengacu pada deskriptor Capsicum spp. International Plant Genetic Resources Institute (IPGRI). Kandungan capsaicin diukur menggunakan metode spektrofotometri, dan profil SSR didapatkan melalui Polymerase Chain Reaction (PCR). Uji statistik dilakukan menggunakan SPSS 16.0 untuk mengatahui signifikansi dari masing-masing karakter kuantitatif. Diversitas genetik direpresentasikan dalam bentuk dendrogram UPGMA menggunakan software NTSYS 2.02i. Lima genotipe yang diamati menunjukkan adanya variasi morfologi berdasarkan karakter kuantitatif dan kualitatif, kecuali warna mahkota. Kelima genotipe memiliki profil SSR yang berbeda-beda antara satu dengan lainnya. CH memiliki kekerabatan paling dekat dengan G1M4. Selain itu, CH juga berkerabat dekat dengan G4 dari profil SSR yang dimiliki. G4 berkerabat dekat dengan G18 dari sifat fenotipe yang dimiliki. Sementara itu, G17 berkerabat jauh dengan genotipe lainnya. Namun, profil SSR yang dimiliki oleh G17 menunjukkan kemiripan yang cukup tinggi dengan profil SSR yang dimiliki oleh G18.
English Abstract
Chili pepper (Capsicum frutescens L.) is one of horticultural plants of the Solanaceae family that is favored by Indonesian people. Chili pepper consists of several varieties that can be found in various regions in Indonesia, including Malang. This study was conducted to describe and analyze genetic diversity of chili pepper G1M4, G4, G17, G18, and CH based on morphological characteristics, capsaicin content, and Simple Sequence Repeat (SSR) profile. The morphological analysis was referred to the descriptors for Capsicum spp. of the International Plant Genetic Resources Institute (IPGRI). The capsaicin content was measured with spectrophotometric methods, and the SSR profile was developed using Polymerase Chain Reaction (PCR). Statistical tests were performed using SPSS 16.0 to determine the significance of each quantitative character. Genetic diversity represented in the form of a dendrogram using NTSYS 2.02i based on UPGMA method. The five genotypes observed showed morphological variations based on quantitative characters and qualitative characters, except the color of the corolla. SSR profiles of each genotype differed from one another. CH has the closest relationship with G1M4. In addition, CH is also closely related to G4 from the SSR profile owned. G4 is closely related to G18 based on phenotypic traits. Meanwhile, G17 is distantly related to other genotypes. However, the SSR profile that is owned by G17 shows a fairly high similarity with the SSR profile owned by G18.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2019/31/051910733 |
| Uncontrolled Keywords: | Cabai rawit, capsaicin, diversitas genetik, SSR, Capsaicin, chili pepper, genetic diversity, SSR |
| Subjects: | 500 Natural sciences and mathematics > 576 Genetics and evolution > 576.5 Genetics > 576.54 Variation > 576.542 Environmental factors (muntagens) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 05 Aug 2020 07:57 |
| Last Modified: | 28 Mar 2022 07:27 |
| URI: | http://repository.ub.ac.id/id/eprint/176744 |
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