Aryani, Nathania (2019) Pengaruh Pemberian Ekstrak Daun Sukun (Artocarpusaltilis)Terhadap Aktivitas Sod Dan Histopatologi Ileum Model Hewan Tikus Putih Inflammatory Bowel Disease (Ibd) Induksi Indometasin. Sarjana thesis, Universitas Brawijaya.
Abstract
Inflammatory Bowel Disease merupakan penyakit yang berkaitan dengan inflamasi pada gastrointestinal dan dapat menimbulkan komplikasi seperti obstruksi, perforasi, dan karsinoma intestinal. IBD dapat diperparah dengan adanya stres oksidatif dari induksi NSAID, terutama indometasin. Adanya kerusakan jaringan dapat dilihat dari penurunan kadar Superoxide Dismutase (SOD) dan perubahan pada mukosa ileum. Daun sukun (Artocarpus altilis) memiliki kandungan flavonoid, tanin, triterpenoid, saponin, alkaloid, dan polifenol yang dapat digunakan sebagai antiinflamasi, antioksidan, dan antidiare. Penelitian ini bertujuan untuk mengkaji pengaruh terapi ekstrak daun sukun terhadap aktivitas Superoxide Dismutase (SOD) dan histopatologi ileum pada tikus Penelitian ini dibagi dalam 5 kelompok tikus yang terdiri dari kelompok A (kontrol negatif), kelompok B (kontrol positif), kelompok P1 (tikus IBD dengan terapi dosis 100 mg/kgBB), kelompok P2 (tikus IBD dengan terapi dosis 200 mg/kgBB), dan kelompok P3 (tikus IBD dengan terapi dosis 400 mg/kgBB). Setelah 14 hari, ileum tikus diambil kemudian dianalisa. Aktivitas enzim Superoxide Dismutase (SOD) diukur dengan menggunakan metode spektofotometri. Gambaran histopatologi ileum diamati secara kualitatif menggunakan mikroskop dengan perbesaran 400x. Analisis data menggunakan One Way ANOVA (Analysis of Varian) dengan taraf kepercayaan 95% dan dilanjutkan dengan uji BNJ. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sukun mampu meningkatkan aktivitas SOD sangat signifikan (P<0.01) hingga mencapai rata-rata 38,38 ± 3,47 dengan peningkatan 69,9%. Gambaran histopatologi menunjukkan bahwa indometasin dapat memicu inflamasi ditandai dengan erosi vili, adanya edema, dan infiltasi sel radang. Pemberian ekstrak daun sukun dapat memperbaiki inflamasi di ileum.
English Abstract
Inflammatory bowel disease is a disease associated with inflammation of the digestive tract and can cause complications such as obstruction, perforation, and intestinal carcinoma. IBD can be aggravated by the presence of oxidative stress from NSAID induction, especially indomethacin. The presence of tissue damage can be seen from decreased levels of Superoxide Dismutase (SOD) and changes in the ileal mucosa. Breadfruit leaves (Artocarpus altilis) contain flavonoids, tannins triterpenoid, saponin, alcaloid,and polyphenols which can be used as anti-inflammatory, antioxidant, and antidiarrheal. This study is aimed to examine the interaction of leaf extracts against Superoxide Dismutase (SOD) activity and ileal histopathology in rats. This study was divided into 5 groups of rats consisting of group A (negative control), group B (positive control), group T1 (IBD rats with therapy dose 100 mg / kgBB), group T2 (IBD rats with therapeutic doses of 200 mg / kgBB), and group T3 (IBD rats with therapy dose of 400 mg / kgBB). After 14 days, the rats ileum was taken and then analyzed. The enzyme activity of Superoxide Dismutase (SOD) was obtained using the spectrophotometric method. The qualitative histopathological description of the ileum was updated using a microscope with 400x magnification. Data analysis using One Way ANOVA (Variant Analysis) with a confidence level of 95% and agreed with the BNJ test. The results showed that administration of breadfruit leaf extract was able to increase SOD activity very significantly (P <0.01) to reach an average of 38.38 ± 3.47 with an increase of 69.9%. Histopathological features can cause inflammation characterized by erosion, edema, and inflammatory cell infiltration. Giving breadfruit leaf extract can overcome inflammation in the ileum.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/47/051908985 |
| Uncontrolled Keywords: | IBD, SOD, Artocarpus altilis, histopatologi, IBD, SOD, Artocarpus altilis, histopatology |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.321 Drugs derived from plants |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 19 Jul 2020 05:45 |
| Last Modified: | 21 Oct 2021 03:10 |
| URI: | http://repository.ub.ac.id/id/eprint/176671 |
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