Murhavisa, Sofines (2019) Studi Eksplorasi Rattus Norvegicus Induksi Streptozotosin Yang Diinfeksi Staphylococcus Aureus Terhadap Kadar Relatif Sod Dan Nf-Kb Limpa. Sarjana thesis, Universitas Brawijaya.
Abstract
Pada keadaan diabetes mellitus (DM) fungsi sel imun terganggu, maka resiko masuknya mikroorganisme lebih besar. Mikroorganisme yang sering muncul pada keadaan DM adalah Staphylococcus aureus (S. aureus). Kondisi DM yang diinfeksi S. aureus akan menyebabkan inflamasi karena pengaktifan Nuclear Factor Kappa Beta (NF-KB). Inflamasi dapat menghasilkan radikal bebas seperti Reactive Oxygen Species (ROS), maka tubuh memerlukan antioksidan seperti superoksida dismutase (SOD) untuk mengkompensasi radikal bebas. Tujuan penelitian ini adalah untuk mengetahui pengaruh kadar relatif NF-KB dan SOD pada model hewan diabetes melitus yang diinfeksi Staphylococcus aureus. Penelitian ini bersifat True Experiment Laboratoric Post Control Only Design dengan lima kelompok dan empat ulangan. Pada kelompok satu tikus digunakan sebagai kontrol negatif, kontrol positif (diinduksi 2 ml Staphylococcus aureus 108 CFU/ml) dan kelompok perlakuan diinduksi streptozotosin 45 mg/kg BB dan Staphylococcus aureus masing - masing 105 CFU/ml, 106 CFU/ml, 107 CFU/ml sebanyak 2 ml secara intraperitoneal. Kadar relatif SOD dan NF-KB diukur menggunakan flow cytomery dan data bersifat kuantitatif, kemudian dianalisis menggunakan Uji One Way Analysis of Variance (ANOVA) dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa induksi streptozotosin dan S. aureus tidak terdapat perbedaan yang nyata pada kadar relatif SOD (P>0,05), namun kadar relatif NF-KB berbeda sangat nyata pada antar kelompok perlakuan (P<0,01). Kesimpulan yang didapat adalah induksi streptozotosin dan S. aureus tidak memengaruhi kadar relatif SOD tetapi dapat meningkatkan kadar relatif NF-KB.
English Abstract
In the case of diabetes mellitus (DM) the function of immune cells is disturbed, so the risk of microorganism’s entry are higher. Microorganism that often appear in DM are Staphylococcus aureus (S. aureus). Diabetes mellitus S. aureus infection will cause inflammation due to activation of Nuclear Factor Kappa Beta (NF-KB). Inflammation produce free radicals such as Reactive Oxygen Species (ROS), so the body needs antioxidants like superoxide dismutase (SOD) to compensate the free radicals. The purpose of this study was to determine the effect of the relative levels of NF-KB and SOD DM with Staphylococcus aureus infection rats. This research is True Experimental Laboratory Post Control Only Design with five groups and four replications. In group one rats were used as negative controls, positive control (induced 2 ml of Staphylococcus aureus 108 CFU / ml and the treatment group streptozotosin induced 45 mg / kg BW and S. aureus respectively 105 CFU/ml, 106 CFU/ml, 107 CFU/ml as much as 2 ml intraperitoneally. Relative levels of SOD and NF-KB were measured using flow cytomery and quantitative data, then analyzed using the One Way Test of Variance (ANOVA) with 95% confidence level. The results showed that streptozotosin and S. aureus had no significant difference in the relative levels of SOD, but the relative levels of NF-KB showed significant differences in each treatment group. The conclusions obtained were that streptozotosin and S. aureus induction did not affect the relative levels of SOD but could increase the relative levels of NF-KB
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2019/46/051908984 |
| Uncontrolled Keywords: | Antioksidan, Diabetes Melitus, Flora Normal, Nuclear Factor kappa Beta (NF-KB), Antioxidants, Diabetes Mellitus, Normal Flora, Nuclear Factor kappa Beta (NF-KB) |
| Subjects: | 500 Natural sciences and mathematics > 599 Mammalia (Mammals) > 599.3 Miscellaneous orders of Eutheria (placental mammals) |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Nur Cholis |
| Date Deposited: | 12 Nov 2020 23:14 |
| Last Modified: | 21 Oct 2021 03:09 |
| URI: | http://repository.ub.ac.id/id/eprint/176666 |
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