Pricilia, Sela (2019) Uji Ekstrak Etanol Daun Inggu (Ruta angustifolia (L.) Pers) sebagai Antifungi terhadap Candida albicans secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Candidaa albicansamerupakan penyebab infeksia oportunistik tersering yang menyerang manusia khususnya pada keadaana imunodefisiensi. Kandidiasisaoral merupakan suatuainfeksi yang paling sering dijumpai dalamaronggaamulutamanusia, dengan prevalensi 40% di Indonesia. Kandidiasisasistemik menyebabkan peningkatan angka kematian sekitar 71%- 79%. Sementara itu, salah satu terapi utama untukakandidiasis ini sudah banyak dilaporkan mengalamiaresistensi. Pengobatan alternatif yang efektif terus dicari untuk menanggulangiaresistensi ini. Salah satunya adalah kandungan saponin dan tanin yang terdapat dalam daun inggu (Rutaaangustifolia (L.) Pers) yang telah diketahui sebagai senyawaaantifungi yang efektif. Penelitian ini bertujuan untuk mengetahuiaefektivitasaekstrakaetanol daunainggu terhadap pertumbuhanafungi Candidaaalbicansasecaraain-vitro. Sampel diperoleh dari stokafungi milikaLaboratoriumaMikrobiologi FKUB. Konsentrasi ekstrak yang dipakai yaitu 0%, 15%, 17,5%, 20%, 22,5% dan 25%. Metode yang digunakan adalahametode dilusi tabung. Hasil uji one way ANOVA menunjukkan adanya perbedaan yang signifikan pada perubahan konsentrasi ekstrakadaunainggu terhadap jumlah koloniaCandidaaalbicansa (p<0,05). Uji korelasi menunjukkan bahwa ekstrakaetanol daunainggu menghambat pertumbuhanakoloni Candidaaalbicansa(Korelasi, r = -0,822: p<0,05). Kesimpulan pada penelitian ini yaitu ekstrak etanol daunainggu (Rutaaangustifolia (L.) Pers) mempunyai efek antifungi terhadap Candidaaalbicansadengan kadar bunuh minimumnya adalah 25%, sementara kadar hambat minimumnya tidak dapat ditentukan dengan metode dilusi tabung. Kata kunci: Candida albicans, ekstrak etanol daun inggu (Ruta angustifolia (L.) Pers), antifungi. vi ABSTRACT Candida albicans is the most common cause of opportunistic infection in human especially in immunodeficiency person. Oral candidiasis is the most common manifestation in human, its prevalence in Indonesia is 40%. Having systemic candidiasis increases the mortality rate to 71%-79%. Instead of that, one of the ultimate therapy that used to cure candidiasis has developed resistance. Therefore, an effective antifungal therapy is in the search. Inggu’s leaf which contain saponin and tannin have been known to be an effective antifungal compounds. This study was aimed to determine the effectiveness of inggu’s leaf ethanol extracts to inhibit the growth of Candida albicans in vitro. The samples were obtained from fungal stock provided by the microbiology laboratory of the School of Medicine Brawijaya University. The concentrations of extract used were 0%, 15%, 17,5%, 20%, 22,5% and 25%. The used method was the tube dilution. The statistical test one-way ANOVA showed significant differences in the changes of inggu’s leaf extract concentrations on the Candida albicans colony numbers (p <0.05). The correlation test showed that inggu’s leaf etanol extract inhibit the growth of Candida albicans colony (correlation, r = -0.822: p <0.05). The conclusion is that inggu’s leaf (Ruta angustifolia (L.) Pers) ethanol extract has an antifungal effect against Candida albicans with minimum bactericidal concentration of 25% and the minimum inhibitory concentration can’t be proved with tube dilution method.
English Abstract
Candida albicans is the most common cause of opportunistic infection in human especially in immunodeficiency person. Oral candidiasis is the most common manifestation in human, its prevalence in Indonesia is 40%. Having systemic candidiasis increases the mortality rate to 71%-79%. Instead of that, one of the ultimate therapy that used to cure candidiasis has developed resistance. Therefore, an effective antifungal therapy is in the search. Inggu’s leaf which contain saponin and tannin have been known to be an effective antifungal compounds. This study was aimed to determine the effectiveness of inggu’s leaf ethanol extracts to inhibit the growth of Candida albicans in vitro. The samples were obtained from fungal stock provided by the microbiology laboratory of the School of Medicine Brawijaya University. The concentrations of extract used were 0%, 15%, 17,5%, 20%, 22,5% and 25%. The used method was the tube dilution. The statistical test one-way ANOVA showed significant differences in the changes of inggu’s leaf extract concentrations on the Candida albicans colony numbers (p <0.05). The correlation test showed that inggu’s leaf etanol extract inhibit the growth of Candida albicans colony (correlation, r = -0.822: p <0.05). The conclusion is that inggu’s leaf (Ruta angustifolia (L.) Pers) ethanol extract has an antifungal effect against Candida albicans with minimum bactericidal concentration of 25% and the minimum inhibitory concentration can’t be proved with tube dilution method.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/380/051910462 |
| Uncontrolled Keywords: | Candida albicans, ekstrak etanol daun inggu (Ruta angustifolia (L.) Pers), antifungi. Candida albicans, inggu’s leaf (Ruta angustifolia (L.) Pers) ethanol extract, antifungal |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.96 Parasitic diseases and diseases due to fungi (mycoses) / Medical parasitology > 616.969 Diseases due to fungi (mycoses) / Mycoses > 616.969 061 Candidiasis--Chemotherapy / Candida albicans--Chemotherapy |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 03 Sep 2020 02:11 |
| Last Modified: | 25 Oct 2021 03:49 |
| URI: | http://repository.ub.ac.id/id/eprint/176336 |
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