Amelya, Elsa (2019) Pengaruh Pemberian Vitamin C Dan Vitamin E Terhadap Jumlah Folikel Pada Tikus (Rattus Norvegicus) Galur Wistar Betina yang diberi Rhodamin B. Sarjana thesis, Universitas Brawijaya.
Abstract
Salah satu faktor yang mempengaruhi infertilitas berasal dari sesuatu yang kita konsumsi seperti makanan. Produsen makanan sebagian besar mencampurkan zat berbahaya seperti Rhodamin B (RB). RB termasuk senyawa xenobiotik (senyawa asing bagi tubuh) yang memiliki produk samping berupa radikal bebas. Solusi untuk menangani masalah radikal bebas perlu adanya antioksidan tambahan dari luar tubuh.Tujuan penelitian ini membuktikan pengaruh pemberian Vitamin C (VC), Vitamin E (VE) dan kombinasi keduanya (VCE) terhadap jumlah folikel tikus galur wistar betina yang diberi rhodamin B (RB). Penelitian ini merupakan penelitian eksperimental yang dilakukan di laboratorium farmakologi FK UB, menggunakan hewan coba tikus galur wistar betina yang dibagi menjadi 5 kelompok : K(-) , K (+), VC, VE dan VCE. Tikus diberi perlakuan selama 36 hari dengan dosis Rhodamin B 90mg/KgBB, vitamin C 600mg/KgBB vitamin E 40IU/KgBB selanjutnya tikus diterminasi dan diambil organ ovariumnya untuk dilakukan pewarnaan HE. Folikel dilihat menggunakan mikroskop Olympus 10 dengan perbesaran 400x. Hasil analisis pada penelitian ini dedapatkan data bersifat homogen (P>0,05), data terdistribusi normal (P>0,05), hasil uji Anova (P>0,05). Hasil pengamatan dari penelitian ini terdapat pengaruh kenaikan dan penurunan pemberian vitamin C dan vitamin E terhadap jumlah folikel tikus yang diberi rhodamin B namun secara statistik tidak terdapat hasil yang signifikan
English Abstract
Infertility is one of the problems in the reproductive health system. One of the factors that affect infertility comes from something we consume like food. Food producers that mostly mix harmful substances such as Rhodamin B (RB). RB includes xenobiotik compounds (foreign compounds for the body) that have a by-product of free radicals. Solutions to overcome the problem of free radicals require additional antioxidants from outside the body. The aim of this study was to prove the effect of giving Vitamin C (VC), Vitamin E (VE) and a combination of both (mice)). This research is an experimental research in the pharmacology laboratory brawijaya university, using experimental animals female wistar rats which are divided into 5 groups: K (-), K (+), VC, VE, and VCE. Mice were treated for 36 days with a dose of Rhodamin B 90 mg / KgBB, vitamin C 600 mg / KgBB of vitamin E 40 IU/ KgBB then the mice were removed surgically and their ovarian organs were taken to stain HE. Follicles are seen using an Olympus 10 microscope with 400x magnification. The results of the analysis in this study obtained homogeneous data (P> 0.05), data were normally distributed (P> 0.05), ANOVA test results (P> 0.05). the results of this study were that there was an effect of increasing and reducing the administration of vitamin C and vitamin E to the number of follicles given rhodamine B but statistically there were no significant results.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2019/114/051909213 |
| Uncontrolled Keywords: | Rhodamin B, Vitamin C, Vitamin E, Jumlah folikel Infertilitas, Rhodamin B, Vitamin C, Vitamin E, Number of follicles |
| Subjects: | 600 Technology (Applied sciences) > 610 Medicine and health > 610.7 Education, research, nursing, services of allied health personnel |
| Divisions: | Fakultas Kedokteran > Kebidanan |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 19 Jul 2020 04:20 |
| Last Modified: | 11 Mar 2022 07:24 |
| URI: | http://repository.ub.ac.id/id/eprint/175238 |
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