Uji Kemampuan Bakteri Pseudomonas fluorescens Sebagai Agens Bioremediasi Insektisida Berbahan Aktif Karbofuran

Lestari, Safira Rizka (2019) Uji Kemampuan Bakteri Pseudomonas fluorescens Sebagai Agens Bioremediasi Insektisida Berbahan Aktif Karbofuran. Sarjana thesis, Universitas Brawijaya.

Abstract

Indonesia dalam memenuhi kebutuhan pangan masih menggunakan sistem tanam monokultur. Sistem tanam monokultur dapat menyebabkan ekosistem menjadi tidak seimbang, sehingga hama dan penyakit sering menyerang. Penggunaan pestisida sintetis menjadi solusi bagi petani. Akan tetapi pestisida, khususnya insektisida bahan aktif karbofuran dapat menghasilkan residu yang susah diuraikan. Terdapat satu upaya untuk mengurangi residu karbofuran yakni dengan melakukan bioremediasi. Bioremediasi merupakan penggunaan mikroorganisme yang ditumbuhkan pada polutan tertentu yang bertujuan untuk mengurangi kadar polutan tersebut (Priadie, 2012). Penelitian uji kemampuan bakteri Pseudomonas fluorescens sebagai agens bioremediasi insektisida berbahan aktif karbofuran ini dilaksanakan di Laboratorium Bakteriologi Penyakit Tumbuhan, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Univeristas Brawijaya dari bulan Desember hingga Januari 2019. Alat yang digunakan dalam penelitian adalah LAFC (Laminar Air Flow Cabinet), autoklaf, spektrofotometer UV-Vis Spectroquant Pharo 300 M, kuvet, cawan petri, pinset, jarum ose, bunsen, tabung reaksi, spatula, labu erlenmeyer, kompor, panci, korek api, kulkas, botol Schott, corong, kertas saring, neraca digital. Bahan yang digunakan dalam penelitian adalah bakteri P. fluorescens kode Pf UB 1 koleksi Jurusan Hama dan Penyakit Tanaman dengan kekeruhan 1,4 x 108 cfu/ml, insektisida berbahan aktif karbofuran, kompos yang berasal dari UPT Kompos Universitas Brawijaya, sampel tanah sawah Lahan Percobaan Fakultas Pertanian Universitas Brawijaya, Desa Jatimulyo, Kecamatan Lowokwaru, Kota Malang, aquades, Nutrient Agar (NA), Nutrient Broth (NB), Plate Count Agar (PCA), aseton, kertas Whatmann No. 1, plastik tahan panas, plastic wrap, aluminium foil, dan tisu. Berdasarkan penelitian yang dilakukan diperoleh P. fluorescens mampu bertahan hidup pada media padat NA yang mengandung 8000 ppm karbofuran. P. fluorescens tidak menghasilkan zona bening dala uji kemampuan degradasi karbofuran. P. fluorescens menyerap karbofuran menjadi sumber karbon serta energi, dan dapat mengurangi konsentrasi karbofuran pada 5 perlakuan yakni 0 ppm, 2000 ppm, 4000 ppm, 6000 ppm, dan 8000 ppm. Penurunan konsentrasi karbofuran berbeda nyata antar perlakuan. P. fluorescens mampu mengurangi konsentrasi karbofuran antara 50-86% selama 15 hari pengamatan.

English Abstract

Indonesia in fulfilling food needs still uses a monoculture planting system. Monoculture planting systems can cause ecosystems to become unbalanced, so that pests and diseases often attack. The use of synthetic pesticides is a solution for farmers. However, pesticides, especially carbofuran active ingredients, can produce residues that are difficult to decipher. There is an effort to reduce carbofuran residue by conducting bioremediation. Bioremediation is the use of microorganisms grown on certain pollutants that aim to reduce the levels of these pollutants (Priadie, 2012). The research on the ability of Pseudomonas fluorescens bacteria as an insecticide bioremediation agent based on carbofuran was carried out in the Plant Bacteriology Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Brawijaya University from December to January 2019. The tool used in the study was LAFC (Laminar Air Flow Cabinet), autoclave, Spectroquant Pharo 300 M UV-Vis spectrophotometer, cuvette, petri dish, tweezers, osseum needle, bunsen, test tube, spatula, erlenmeyer flask, stove, pan, lighters, refrigerator, Schott bottle, funnel, filter paper , digital balance. The material used in the study was P. fluorescens code Pf UB 1 collection of Pests and Plant Diseases with turbidity of 1.4 x 108 cfu / ml, insecticides with active carbofuran, compost originating from UPT Kompos Universitas Brawijaya, rice field samples Experimental Field Brawijaya University Faculty of Agriculture, Jatimulyo Village, Lowokwaru District, Malang City, Aquades, Nutrient Agar (NA), Nutrient Broth (NB), Plate Count Agar (PCA), Acetone, Whatmann No. 1, heatresistant plastic, plastic wrap, aluminum foil, and tissue. Based on the research conducted, P. fluorescens was able to survive on NA solid media containing 8000 ppm carbofuran. P. fluorescens does not produce clear zones in the test of carbofuran degradation ability. P. fluorescens absorbs carbofuran into a carbon source and energy, and can reduce the concentration of carbofuran in 5 treatments, namely 0 ppm, 2000 ppm, 4000 ppm, 6000 ppm, and 8000 ppm. Decreasing carbofuran concentration was significantly different between treatments. P. fluorescens is able to reduce the concentration of carbofuran between 50-86% for 15 days of observation.

Item Type: Thesis (Sarjana)
Identification Number: SKR/FP/2019/411/051907149
Uncontrolled Keywords: -
Subjects: 600 Technology (Applied sciences) > 632 Plant injuries, diseases, pests > 632.9 General topics of pest and disease control
Divisions: Fakultas Pertanian > Agroekoteknologi
Depositing User: soegeng sugeng
Date Deposited: 24 Aug 2020 07:15
Last Modified: 21 Apr 2022 05:45
URI: http://repository.ub.ac.id/id/eprint/173468
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