Daten, Helena (2018) Potensi Penambahan Probiotik (Lactobacillus Pentosus K50) Untuk Meningkatkan Kualitas Pakan Ikan Air Tawar. Sarjana thesis, Universitas Brawijaya.
Abstract
Pakan merupakan sumber nutrisi bagi ikan, dapat diproses dengan penambahan probiotik. Probiotik merupakan mikroba yang berperan untuk meningkatkan kesehatan inang dan kualitas pakan. Penelitian ini bertujuan untuk mengetahui viabilitas bakteri asam laktat (BAL), jumlah bakteri Salmonella dan coliform, kapang, serta kandungan nutrisi pakan. Perlakuan fermentasi terdiri dari tanpa penambahan BAL (kontrol), penambahan BAL Sp. 1, probiotik (Lactobacillus pentosus K50), dan bakteri konsorsium (Lactobacillus pentosus K50 dan BAL Sp. 1). Jumlah BAL, Salmonella dan coliform serta kapang dideteksi dengan metode Total Plate Count. Kandungan protein diuji menggunakan metode Kjedahl, karbohidrat dengan metode total carbohydrate by difference, dan lemak, abu, serta air dengan metode gravimetri. Data dianalisis menggunakan one way ANOVA selang kepercayaan 95 %. Viabilitas BAL pada perlakuan penambahan BAL Sp.1 mengalami penurunan dari hari ke-0 sampai 20, sedangkan perlakuan dengan penambahan Lactobacillus pentosus K50 dan konsorsium meningkat dari hari ke- 0 sampai 20 berturut-turut 12,6 x 108 CFU/g menjadi 17,4 x 108 CFU/g dan 11,2 x 108 CFU/g menjadi 14,9 x 108 CFU/g. Bakteri Salmonella tidak tumbuh dalam pakan kontrol dan fermentasi. Jumlah bakteri coliform dan kapang dalam pakan terfermentasi dengan perlakuan probiotik dan bakteri konsorsium mengalami penurunan hari ke-5 sampai 20. Jumlah kapang pada perlakuan dengan penambahan BAL Sp. 1 mengalami fluktuasi disebabkan meningkatnya kadar air selama fermentasi. Kandungan protein relatif stabil, sedangkan kandungan lemak, karbohidrat, dan abu mengalami penurunan selama fermentasi. Penambahan probiotik dalam pakan dapat menghambat pertumbuhan bakteri coliform dan kapang, serta menjaga stabilitas nutrisi pakan.
English Abstract
Feed is the nutrition sources for fish which could be processed by apply probiotic. Probiotic is microbe applied to improve the health of host and feed quality. The objective of this study was to observe the viability of lactic acid bacteria (LAB), number of Salmonella, coliform and mold also contain of feed nutrition. Fermentation treatment consist of feed without application of LAB (control), application of LAB Sp. 1, probiotic (Lactobacillus pentosus K50) and consortium bacteria (Lactobacillus pentosus K50 and LAB Sp. 1). Number of LAB, Salmonella, coliform, and mold was detected using Total Plate Count method. Protein content was assayed using Kjedahl method, carbohydrate content was assayed using total carbohydrate by difference method, and fat, ash also water content were assayed using gravimetric method. Data were analyzed using one way ANOVA with confidence level 95 %. Viability of LAB with application of LAB Sp. 1 decreased 0 to 20 days, while application of Lactobacillus pentosus K50 also consortium bacteria increased viability 0 to 20 days from 12,6 x 108 CFU/g to 17,4 x 108 CFU/g and 11,2 x 108 CFU/g to 14,9 x 108 CFU/g, respectively. Salmonella bacteria did not in all treatment fermented feeds. Number of coliform bacteria and mold in fermented feed under treatment of probiotic also consortium bacteria decreased 5 to 20 days. Number of mold in application of LAB Sp. 1 was fluctuated due to the increasing of water content during fermentation. However, protein content was relatively stable. While fat, carbohydrate, and ash content were decreased during fermentation. Application of probiotic inhibits coliform bacteria and mold growth, also maintain the stability of feed nutrition.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/154/051805300 |
| Uncontrolled Keywords: | fermentasi, kualitas pakan, pakan, probiotik, protein/ fermentation, feed quality, feed, probiotic, protein |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.08 Specific topics in animal husbandry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Endang Susworini |
| Date Deposited: | 15 Jul 2020 08:18 |
| Last Modified: | 15 Jul 2020 08:18 |
| URI: | http://repository.ub.ac.id/id/eprint/168988 |
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