Dipratama, Arnoldus Reynaldo (2018) Potensi Metabolit Rhizobacteria Sambiloto (Andrographis Paniculata) Sebagai Antibakteri Salmonella Typhi Atcc 58105535. Sarjana thesis, Universitas Brawijaya.
Abstract
Salmonella Typhi merupakan bakteri penyebab demam tifoid yang dapat diobati dengan pemberian antibiotik, namun bakteri tersebut menunjukkan resisten terhadap beberapa antibiotik. Salah satu tanaman obat yang dapat digunakan untuk mengobati demam tifoid adalah Andrographis paniculata yang mengandung senyawa antibakteri. Tujuan penelitian ini untuk menganalisis daya hambat metabolit rhizobacteria A. paniculata terhadap S. Typhi ATCC 58105535 dan mengidentifikasi spesies bakteri unggul. Metode penelitian meliputi isolasi rhizobacteria A. paniculata menggunakan teknik dilusi, skrining rhizobacteria dan uji potensi metabolit bakteri terhadap S. Typhi ATCC 58105535 menurut metode disc diffusion, dan identifikasi rhizobacteria unggul berdasarkan sekuen 16S rDNA. Tanah rizosfer A. paniculata terdapat 21 isolat bakteri, dua isolat yaitu SL7 dan SL18 menunjukkan daya hambat tertinggi terhadap S. typhi ATCC 58105535 dengan diameter zona hambat secara berurutan adalah 8,31±2,6 dan 8,05±0,58 mm. Metabolit cell-free supernatant (CFS) isolat SL7 lebih unggul menghambat S. typhi ATCC 58105535 dengan diameter zona hambat 7,33±0,08 mm dibandingkan SL18. Isolat SL7 merupakan Bacillus wiedmannii FSL W8-0169 dengan nilai similaritas sekuen 16S rDNA sebesar 100 %. Isolat SL7 dapat dikembangkan sebagai agen penghasil metabolit antibakteri patogen
English Abstract
Salmonella Typhi causes typhoid fever disease which can be treated by using antibiotics, however S. typhi has been resistant to common antibiotics. One of medicinal plants which can be used to treat typhoid fever is Andrographis paniculata that contain antibacteria substance. The purposes of this research were to analyze the inhibiting ability of rhizospheric bacteria metabolites of A. paniculata against S. Typhi ATCC 58105535 and to identify the best bacteria species to inhibit S. Typhi ATCC 58105535. The methods were isolation of A. paniculata’s rhizobacteria using dilution technique, screening test of rhizobacteria and potency assay of bacterial metabolites against S. Typhi ATCC 58105535 using disc diffusion method, and molecular identification of the best rhizobacteria based on 16S rDNA sequence. There were 21 different isolates obtained from rhizosphere of A. paniculata. Two isolates were SL7 and SL18 showed the highest activity (8,31±2,6 and 8,05±0,58 mm diameter) respectively. The metabolite of cell-free supernatant (CFS) of SL7 had better ability than SL18 to inhibit S. Typhi ATCC 58105535, which had 7,33±0,08 mm diameter. Isolate of SL7 with Bacillus wiedmannii FSL W8-0169 had 100 % of similarity value of 16S rDNA sequence. Isolate SL7 can be developed as antibacterial metabolites producing agent against pathogen.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/431/051811084 |
| Uncontrolled Keywords: | Andrographis paniculata, antibiotik, metabolit, rizosfer, Salmonella Typhi, Andrographis paniculata, antibiotic, metabolites, rhizosphere, Salmonella Typhi |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 06 Jun 2020 09:44 |
| Last Modified: | 23 Oct 2021 07:26 |
| URI: | http://repository.ub.ac.id/id/eprint/168810 |
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