Kartikasari, Rr. Intan (2018) Eksplorasi Bakteri Asam Laktat Dari Saluran Pencernaan Kerang Pisau (Solen Spp.) Yang Berpotensi Sebagai Probiotik Di Pesisir Selatan Kabupaten Bangkalan, Madura. Sarjana thesis, Universitas Brawijaya.
Abstract
Kerang pisau termasuk dalam Famili Solenidae yang banyak ditemukan di pesisir pantai berlumpur. Kerang tersebut mudah terserang penyakit yang disebabkan oleh bakteri patogen Vibrio harveyi. Salah satu upaya untuk mengatasi hal tersebut dengan pemanfaatan bakteri probiotik yang mampu menghambat pertumbuhan bakteri patogen. Tujuan dari penelitian ini adalah mengkarakterisasi bakteri asam laktat (BAL) dari saluran pencernaan kerang pisau yang berpotensi sebagai probiotik selanjutnya dilakukan identifikasi bakteri tersebut. Isolasi BAL dilakukan menggunakan medium De Man Rogosa Sharpe (MRS) agar yang ditambahkan 0,004% bromocresol purple (BCP), 1% CaCO3 dan 0,1% nystatin (100.000 Unit). Selanjutnya dilakukan karakterisasi meliputi pewarnaan Gram, aktivitas katalase, penghambatan terhadap Vibrio harveyi, toleransi terhadap asam (pH 3 & 4) dan garam empedu (0,3%, 0,5%, & 1%). Isolat terpilih diidentifikasi berdasarkan sekuen 16S rDNA. Hasil yang diperoleh terdapat 22 isolat BAL dengan karakteristik Gram positif & katalase negatif. Kedua isolat (SP5 dan SP8) mampu menghambat Vibrio harveyi dengan zona hambat berturut-turut sebesar 2,5 ± 0,5 dan 2,8 ± 1,9 mm. Kedua isolat tersebut memiliki survival rate >100% pada pH 4. Sedangkan pada pH 3 isolat SP8 yang memiliki survival rate tertinggi yaitu 99%. Uji toleransi terhadap garam empedu, survival rate tertinggi pada konsentrasi 0,3% ditunjukkan oleh isolat SP5 dan SP8 sebesar 99%, sedangkan pada konsentrasi 0,5% dan 1% ditunjukkan oleh isolat SP5. Kedua isolat tersebut memiliki similaritas 100% dengan Lactobacillus plantarum
English Abstract
Razor clams (Solen spp.) belong to Family Solenidae that are found at the muddy coast. Razor clams easily infected by pathogenic bacteria such as Vibrio harveyi. One effort to overcome this infection is the presence of probiotic bacteria that can inhibit the growth of the pathogenic bacteria. The objectives of this research were to characterize lactic acid bacteria (LAB) from digestive tract of razor clams that have potential as probiotics, then identification of the isolates. Lactic acid bacteria isolation was performed using De Man Rogosa Sharpe (MRS) medium with 0.004% bromocresol purple (BCP), 1% CaCO3 and 0.1% nystatin (100.000 Unit). The isolate were tested the Gram staining, catalase activity, inhibition of Vibrio harveyi, tolerance to acids (pH 3 & 4) and bile salts (0.3%, 0.5%, & 1%). Selected LAB were identified by according to the 16S rDNA sequence. There are 22 isolates of LAB that characterized as Gram positive and catalase negative. Two isolates (SP5 and SP8) were able to inhibit Vibro harveyi with inhibition zone of 2,5 ± 0,5 and 2,8 ± 1,9 mm, respectively. The isolates had survival rate > 100% at pH 4 medium. While at pH 3 only isolate S8 has the highest survival rate (99%). At bile-salt tolerant test, the highest survival rate at 0.3% concentration was indicated by SP5 and SP8 isolates that were equal to 99%, while at concentrations of 0.5% and 1% were indicated by SP5 isolate. Both isolates have a similarity of 100% with Lactobacillus plantarum.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/258/051807071 |
| Uncontrolled Keywords: | BAL, garam empedu, pH, Solen spp., Vibrio harveyi, Bile salt, LAB, pH, Solen spp., Vibrio harveyi |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.3 Prokaryotes |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Biologi |
| Depositing User: | Nur Cholis |
| Date Deposited: | 06 Jun 2020 09:35 |
| Last Modified: | 22 Oct 2021 08:05 |
| URI: | http://repository.ub.ac.id/id/eprint/168788 |
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