Dewantoro, Bhisma Alfathan (2018) Pengaruh Ekstrak Kulit Jeruk Lemon (Citrus Limon) terhadap Bakteri Escherichia coli secara In Vitro. Sarjana thesis, Universitas Brawijaya.
Abstract
Escherichia coli merupakan bakteri patogen utama infeksi pada pasien rawat jalan maupun rawat inap. Sekitar 85% penyebab ISK (infeksi saluran kemih) dan sekitar 50% infeksi nosokomial di masyarakat penyebabnya adalah Escherichia coli. Infeksi nosokomial yang sering disebabkan oleh Escherichia coli seperti catheter-associated urinary tract infections dan infeksi bekas luka operasi. Penularan bakteri ini sangat mudah, yaitu dari tangan yang menyentuh makanan atau air yang telah terkontaminasi dan menyebabkan adanya transfer gen. Jeruk lemon (Citrus limon) merupakan salah satu tanaman yang banyak dan sering di budidayakan, baik untuk penyedap masakan, kecantikan maupun obat. Jeruk lemon (Citrus limon) kaya akan senyawa alam berupa asam sitrat, asam askorbik, mineral, flavonoid dan minyak esensial. Penelitian ini bertujuan untuk mengetahui potensi antimikroba ekstrak kulit jeruk lemon (Citrus limon) terhadap Escherichia coli dengan menggunakan metode difusi sumuran. Konsentrasi ekstrak kulit jeruk lemon yang digunakan adalah 15%, 20%, 25%,30%, dan 35% dengan empat kali pengulangan. Berdasarkan hasiI peneIitian, didapatkan zona inhibisi terbentuk pada seluruh konsentrasi uji tersebut. Hasil uji One-Way ANOVA menunjukkan ada perbedaan efek yang bermakna pada perubahan konsentrasi ekstrak etanol kulit jeruk lemon terhadap zona inhibisi pertumbuhan bakteri Escherichia coli (p<0.05). Uji korelasi Spearman menunjukkan adanya terdapat hubungan yang bermakna antara pemberian ekstrak dengan diameter zona hambat . Berdasarkan penelitian ini, dapat disimpulkan bahwa ekstrak kulit jeruk lemon memiliki potensi sebagai antimikroba terhadap bakteri Escherichia coli secara in vitro.
English Abstract
Escherichia coli is the major pathogen bacteria infection in outpatient or inpatient. Approximately 85% of the causes of UTI (urinary tract infection) and about 50% of nosocomial infections in the Community cause is Escherichia coli. Nosocomial infections are often caused by Escherichia coli as a catheter-associated urinary tract infections, and infections of bacterial Transmission operation scar is very simple, namely from the hands that touch food or water that has been contaminated and caused the presence of gene transfer. lemon (Citrus limon) is the one of many plant that got a lot of cultivation,like flavoring dishes, beauty or drug. Lemon (Citrus limon) is rich in natural compounds in the form of citric acid, askorbik acid, flavonoids, minerals and essential oils. This research aims to know the potential antimicrobial skin extract, lemon (Citrus limon) against Escherichia coli using sumuran diffusion method. The concentration of extract lemon skin used is 15%, 20%, 25% .30%, and 35% with four repetitions. Based on the result, obtained as a zone of inhibition is formed on all the test concentrations. The results of the test of one-way ANOVA showed no difference in effect meaning on the change of concentration of ethanol extract of lemon skin against the zone of inhibition of growth of bacteria Escherichia coli (p < 0.05). Spearman correlation test showed a relationship between the giving of the extract with the diameter of the zones of drag. Based on this research, it can be concluded that the extract of lemon skin has potential as an antimicrobial against Escherichia coli bacteria
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/631/051902099 |
| Uncontrolled Keywords: | Escherichia coli, kulit jeruk lemon, potensi antimikroba-Escherichia coli, lemon skin, antimicrobial potency |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.323 77 Drugs derived from spesific plants (Citrus) |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 05 Jul 2019 03:18 |
| Last Modified: | 05 Jul 2019 03:18 |
| URI: | http://repository.ub.ac.id/id/eprint/168356 |
Actions (login required)
 |
View Item |