Bramantyo, Mochammad Hafid (2018) Penentuan Kondisi Optimum Xilanase dari Trichoderma viride yang Diamobilkan pada Matriks Bentonit Ca-alginat. Sarjana thesis, Universitas Brawijaya.
Abstract
Xilanase tergolong enzim ektrasesluler yang dapat menghidrolisis xilan menjadi xilosa. Enzim Xilanase dapat digunakan secara efisien dan berulang, apabila telah diamobilkan. Metode amobilisasi yang digunakan adalah metode adsorbsi fisik menggunakan bentonit dilanjutkan metode entrapment Ca-alginat. Penelitian ini bertujuan untuk mengetahui kondisi kerja optimum xilanase yang diamobilkan pada matriks bentonit Ca-alginat meliputi pH, temperatur, dan waktu inkubasi. Kondisi optimum ini ditentukan dari pengukuran aktivitas xilanase pada variasi pH ( 3, 4, 5, 6, 7 ), temperatur ( 40, 50, 60, 70, 80 ) °C, dan waktu inkubasi ( 40, 45, 50, 55, 60, 65 ) menit. Data yang diperoleh dianalisis secara statistik dengan menggunakan SPSS 22.0 melalui uji ANOVA one-way dan dilanjutkan dengan uji BNJ (α = 5%). Hasil dari penelitian ini menunjukkan bahwa aktivitas optimum xilanase amobil pada matriks Bentonit Ca-alginat yaitu pada pH 4; temperatur optimum pada 50 °C; dan waktu inkubasi optimum pada 45 menit dengan nilai aktivitas sebesar 17,840 μg/mg.menit.
English Abstract
Xylanase is an extracellular enzyme that capable to hydrolyze xylan to xylose. Xylanase needs to be immobilize to improve it’s efficiency so that can be used in multiple times. The method used are physical adsorption in an activated bentonite, then continued Ca-alginate entrapment. This study aims to determine the optimum conditions of immobilized xylanase in bentonite coated by Ca-alginate including pH, temperature, and incubation time The optimum condition was determined by measuring xylanase activity on pH (3, 4, 5, 6, 7), temperature (40, 50, 60, 70, 80) °C, and incubation time (40, 45, 50, 55, 60, 65) minutesThe data obtained were analyded statistically using SPSS 22.0 through ANOVA one-way test and continued with BNJ test (α = 5%). The results of this study showed that the optimum condition of immobilized xylanase are at pH of 4; with temperature at 50 oC and incubation time at 45 minutes with activity value 17,840 μg/mg.minute.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/MIPA/2018/358/051807411 |
| Uncontrolled Keywords: | Trichoderma viride, Enzim Xilanase, Amobil, Bentonit, Ca-alginat Immobilized Xylanase, Xylanase, Trichoderma viride, Bentonite, Ca-alginate, Ca-alginate Coated Bentonite |
| Subjects: | 500 Natural sciences and mathematics > 579 Natural history of microorganisms, fungi, algae > 579.5 Fungi > 579.56 Ascomycotina ( ascomycetes) > 579.567 Pyrenomycetes > 579.567 7 Hypocreales |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam > Statistika |
| Depositing User: | Budi Wahyono Wahyono |
| Date Deposited: | 17 Jun 2020 07:39 |
| Last Modified: | 18 Oct 2021 03:55 |
| URI: | http://repository.ub.ac.id/id/eprint/168242 |
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