Asri, Khairiza (2018) Studi Perbandingan Tingkat Stres Lutung Jawa dengan Mengukur Kadar Hormon Kortisol, Rasio Neutrosil Per Limfosit di Kandang Perawatan dan Kandang Karantina di Javan Langur Center. Sarjana thesis, Universitas Brawijaya.
Abstract
Lutung Jawa (Trachypithecus auratus) merupakan salah satu satwa yang dilindungi. Lutung Jawa termasuk dalam status vulnerable (terancam punah) dalam IUCN dan terdaftar dalam Appendix II (tidak boleh diperdagangkan) dalam CITES. Javan Langur Center merupakan tempat rehabilitasi lutung Jawa yang bertujuan untuk mencegah penurunan populasi dihabitat aslinya. Lutung Jawa yang hidup di rehabilitasi rentan terhadap kondisi stres. Stres merupakan suatu kondisi yang dapat menimbulkan gangguan homeostasis pada hewan sehingga menyebabkan dampak negatif terhadap kesejahteraan hewan. Penelitian ini bertujuan untuk mengkaji terjadinya gejala stres melalui pengukuran rasio neutrofil:limfosit dan hormon kortisol pada lutung Jawa (Trachypithecus auratus) sebagai indikator stres di kandang perawatan dan kandang karantina. Pada penelitian ini digunakan 5 ekor lutung Jawa betina dan jantan berumur 2 hingga 8 tahun di Kawasan Javan Langur Center (JLC) untuk diambil sampel darahnya. Pengukuran konsentrasi hormon kortisol menggunakan serum darah dengan enzyme-linked immunosorbent assay (ELISA) dan pengukuran rasio neutrofil : limfosit menggunakan whole blood dilakukan dengan uji hematologi. Hasil penelitian menunjukkan bahwa sampel pada kandang perawatan dan kandang karantina memiliki kadar kortisol rata-rata 109,693 ng/mL dan 104,418 ng/mL, dan pengukuran rasio Neutrofil/Limfosit dikandang perawatan dan kandang karantina memiliki rata-rata 8,24 dan 2,4. Terdapat perbedaan kadar kortisol dan rasio neutrofil per limfosit pada kandang perawatan dan kandang karantina.
English Abstract
Javan langur (Trachypithecus auratus) is one of the protected animals. Javan langur is classified as vulnerable (endangered) on the IUCN and listed as Appendix II (prohibited to be traded) on CITES. Javan Langur Center is a rehabilitation place that has the purpose to prevent population decline of native wild animals in their habitat. Javan langurs that live in captivity are susceptible to a stressful condition. Stress is a condition that can cause homeostasis imbalance in animals, causing negative impact on animal welfare. The aim of this study was to review the occurrence of stress symptoms through measurement of the ratio of neutrophils:lymphocytes and cortisol hormones in Javan langur (Trachypithecus auratus) as a stress indicator in treatment cage and quarantine cage. This research used blood sample of 5 female and male Javan langurs age 2 to 8 years old, at the Javan Langur Center (JLC). The measurement of cortisol hormone concentrations in serum by enzyme-linked immunosorbent assay (ELISA) method and the ratio of neutrophil:lymphocytes in whole blood was measured by hematology test. The result showed, average cortisol level in treatment cage and quarantine cage was 109,693 ng/mL and 104,418 ng/mL, and average neutrophil and lymphocyte ratio in treatment cage and quarantine cage was 8,24 and 2,4. There was a difference in cortisol levels and the ratio of neutrophils per lymphocyte between treatment cage and quarantine cage.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/49/051805216 |
| Uncontrolled Keywords: | Stres, Lutung Jawa, Kortisol, Neutrofil per Limfosit, Kandang karantina / Stress, Javan langur, Cortisol, Neutrophil and Lymphocytes, Quarantine cage |
| Subjects: | 600 Technology (Applied sciences) > 636 Animal husbandry > 636.9 Other mammals |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 22 May 2020 11:39 |
| Last Modified: | 22 May 2020 11:39 |
| URI: | http://repository.ub.ac.id/id/eprint/167912 |
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