Syahputra, Rizal Pandu (2018) Potensi Terapi Topikal VCO (Virgin Coconut Oil ) pada Tikus (Rattus norvegicus) Model Luka Bakar Derajat II B terhadap Kadar TNF-α dan Ketebalan Epidermis. Sarjana thesis, Universitas Brawijaya.
Abstract
Luka bakar dapat menyebabkan kerusakan secara lokal dan sistemik hingga dalam skala tertentu dapat berujung pada kematian. Pengobatan luka bakar umumnya menggunakan ekstrak plasenta yang memiliki kemampuan mempercepat re-epitelisasi. VCO (Virgin Coconut Oil) memiliki potensi sebagai terapi luka bakar derajat II B karena VCO mengandung phytosterol sebagai antiinflamasi, tokoferol sebagai antioksidan dan asam lemak bebas sebagai antimikrobial serta mampu mempercepat metabolisme. Penelitian ini bertujuan untuk mengetahui perbedaan respon antara sediaan topikal ekstrak plasenta dan VCO dalam penyembuhan luka bakar derajat II B. Penelitian bersifat eksperimental menggunakan post test only two group experimental design, dua perlakuan yaitu kelompok perlakuan terapi ekstrak plasenta dan kelompok perlakuan terapi VCO. Induksi luka bakar dilakukan pada area flank tikus (Rattus norvegicus) dengan luas 2x2cm. Analisa kadar TNF-α dengan Sandwich ELISA sedangkan ketebalan epidermis diwarnai dengan pewarnaan HE kemudian dianalisa secara mikroskopis. Data dianalisa secara kuantitatif menggunakan uji T Independen. Hasil penelitian menunjukan bahwa tidak terdapat perbedaan yang signifikan (p>0,05) antara kelompok terapi salep VCO dan kelompok terapi gel ekstrak plasenta pada luka bakar derajat II B berdasarkan kadar TNF-α dan ketebalan epidermis. Kesimpulan penelitian ini salep VCO dapat digunakan sebagai terapi pada luka bakar derajat II B.
English Abstract
Burn wound can cause local and systemic damage until the certain scale which can lead to death. Currently, the treatment of burns generally use placenta extract which has the ability to accelerate the re-epithelization. VCO (Virgin Coconut Oil) has the potential to be used as second-degree burn therapy because VCO contains phytosterol which serves as anti-inflammatory, tocopherol which has a function as antioxidant and free fatty acid which have antimicrobial activity It is also able to speed up metabolism. The objective of this research was to know the difference response of topical agent between placenta extract and VCO in healing II B degree burn wound. This study used post test only two experimental group design, 2 treatment group those were treated with placenta extract and VCO. The induction of the burn wound was created on the flank of rat (Rattus norvegicus) as wide as 2x2cm. The expression of TNF-α was analyzed with Sandwich ELISA, while the thickness of the epidermis was stained with HE, then analyzed microscopically. The data were analyzed quantitatively using Independent T test. The result showed that was no significant differences (p>0.05) between therapeutic group of VCO ointment and therapeutic group of placenta extract gel on II B degree burn wound toward TNF-α level and the epidermis thickness. It could be concluded that topical therapeutic of VCO ointment could be used as topical therapy of II B degree burn wound.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FKH/2018/70/051805237 |
| Uncontrolled Keywords: | Luka bakar, VCO, TNF-α, Ketebalan Epidermis / Burn wound, VCO, TNF-α, Epidermis Thickness |
| Subjects: | 600 Technology (Applied sciences) > 617 Surgery, regional medicine, dentistry, ophthalmology, otology, audiology > 617.2 Results of injuries and wounds |
| Divisions: | Fakultas Kedokteran Hewan > Kedokteran Hewan |
| Depositing User: | Endang Susworini |
| Date Deposited: | 18 May 2020 03:05 |
| Last Modified: | 18 May 2020 03:05 |
| URI: | http://repository.ub.ac.id/id/eprint/167905 |
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