Putra, Gumilar Fardhani Ami (2018) Efektivitas Ekstrak Etanol Kulit Salak Pondoh (Salacca Zalacca) Sebagai Antimikroba Terhadap Bakteri Vibrio Cholerae. Sarjana thesis, Universitas Brawijaya.
Abstract
Bakteri Vibrio cholerae adalah penyebab penyakit kolera. Penyakit kolera menyebabkan diare yang dapat menimbulkan rejatan hipovolemik, asidosis metabolik, hingga menyebabkan kematian. Kolera pernah menjadi pandemi di Indonesia pada tahun 1961. Di tahun 2016, 132.121 kasus kolera tercatat oleh World Health Organization (WHO). Pada tahun 2018 sendiri, terjadi wabah kolera di Nigeria dengan 8535 kasus tercatat oleh WHO. Bakteri kolera juga ditemukan resisten terhadap ampicillin, kotrimoksasol, gentamisin, dan tetrasiklin di beberapa negara, sehingga dibutuhkan pengobatan alternatif untuk penyakit kolera. Salak merupakan tanaman buah ketiga terbanyak di Indonesia berdasarkan data dari Kementrian Pertanian Indonesia. Penelitian ini bertujuan untuk mengetahui kadar hambat minimum dari ekstrak etanol kulit salak pondoh terhadap Vibrio cholerae, serta hubungan perbedaan konsentrasi ekstrak dengan pertumbuhan bakteri. Metode yang dipakai adalah dilusi agar dengan 6 konsentrasi berbeda, yaitu 0%, 9%, 10%, 11%, 12%, dan 13%. Hasil penelitian dianalisis dengan uji saphiro-wilk, uji Mann Whitney, uji Kruskal Wallis, dan uji korelasi Spearman. Pemberian ekstrak kulit salak mengakibatkan perbedaan pertumbuhan bakteri (p = 0,000). Pertumbuhan bakteri kolera dihambat pada konsentrasi ekstrak 12%. Uji korelasi menunjukkan korelasi negatif (korelasi = -0,926) yang berarti makin tinggi konsentrasi ekstrak, makin rendah pertumbuhan bakteri. Dapat disimpulkan bahwa ekstrak etanol kulit salak pondoh memberikan efek antimikroba terhadap bakteri Vibrio cholerae.
English Abstract
Vibrio cholerae is the causative agent of cholera. Cholera manifest as diarrhea that leads to hypovolemic shock, metabolic acidosis, and eventually death. Cholera had been a pandemic disease in Indonesia in 1961. In 2016, 132.121 cases of cholera had been recorded by the World Health Organization (WHO). In 2018, cholera outbreak occurs in Nigeria with 8535 cases recorded by WHO. Vibrio cholerae had been reported to be resistant to ampicillin, cotrimoxazole, gentamicin, and tetracyclin in some countries, so alternative treatment is needed. Snake fruit is the third most abundant fruit in Indonesia based on the data by Agriculture Ministry of Indonesia. This study aimed to discover the minimum inhibitory concentration of snake fruit bark ethanol extract against Vibrio cholerae, and also the correlation between different concentrations of extract with bacterial growth. Method used is agar dilution with 6 different concentrations, 0%, 9%, 10%, 11%, 12%, and 13%. Study result analyzed with saphiro wilk test, Mann Whitney test, Kruskal Wallis test, and Spearman correlation test. Snake fruit bark extract caused difference in bacterial growth (p = 0,000). Cholera bacterial growth inhibited at 12%. Correlation test showed negative correlation (value = -0,926) which means the higher the extract’s concentration, the lower bacterial growth will. Snake fruit bark ethanol extract have antimicrobial effect against Vibrio cholerae.
Other obstract
-
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/610/051901705 |
| Uncontrolled Keywords: | Kulit Salak Pondoh, Vibrio cholerae, Kolera, Snake fruit bark, Vibrio cholerae, Cholera |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.93 Clostridium infections, diphtheria, cholera, dysenteries, protozoan infections > 616.932 Cholera |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 24 May 2020 10:21 |
| Last Modified: | 21 Oct 2021 04:34 |
| URI: | http://repository.ub.ac.id/id/eprint/167876 |
Preview |
Text
Gumilar Fardhani Ami Putra.pdf Download (3MB) | Preview |
Actions (login required)
 |
View Item |