Aninditha, Dea (2018) Pengaruh Antibodi Molekul Adhesin 49,8 Kda Sub Unit Pili Shigella flexneri Terhadap Efek Aglutinasi Molekul Adhesin Vibrio cholerae. Sarjana thesis, Universitas Brawijaya.
Abstract
Di Indonesia, 14 dari 34 provinsi memiliki prevalensi diare yang lebih besar dibanding prevalensi diare nasional. Etiologi diare bisa bermacam-macam, termasuk Shigella flexneri dan Vibrio cholerae. Pada tahun 2017, terjadi wabah kolera di Yaman yang menyebabkan 2.000 dari 500.000 penderitanya meninggal dunia. Banyaknya kasus diare yang berujung fatal menyebabkan dibutuhkannya suatu usaha pencegahan seperti vaksinasi. Vaksin berbasis komponen patogen dianggap merupakan bentuk vaksin yang paling aman. Molekul adhesin subunit pili 49,8 kDa Shigella flexneri dan molekul adhesin subunit pili 37,8 kDa Vibrio cholerae merupakan protein hemaglutinin yang berperan dalam proses infeksi. Penelitian ini bertujuan untuk mengetahui reaksi silang atau penghambatan efek hemaglutinasi molekul adhesin Vibrio cholerae 37,8 kDa setelah diberi antibodi molekul adhesin 49,8 kDa subunit pili Shigella flexneri dengan desain penelitian eksperimen murni melalui uji antihemaglutinasi. Didapatkan hasil positif hingga titer antibodi 1/64. Berdasarkan penelitian ini, dapat disimpulkan bahwa terjadi reaksi silang antara molekul adhesin subunit pili Shigella flexneri 49,8 kDa dengan molekul adhesin subunit pili Vibrio cholerae 37,8 kDa sehingga dapat dijadikan pertimbangan dalam pembuatan vaksin yang homolog.
English Abstract
In Indonesia, 14 out of 34 provinces had higher prevalence of diarrhea than the national prevalence. The etiology could be various including Shigella flexneri and Vibrio cholerae. In 2017, there was an outbreak of Cholera in Yemen with 500.000 people being infected and 2.000 people died. With an overwhelming number of death, we need to prevent this event through vaccination. Vaccine that consist of pathogen’s component considered the safest form. Adhesion mollecule 49,8 kDa of Shigella flexneri and adhesion mollecule 37,8 kDa of Vibrio cholerae are hemagglutinin protein that have a role in infection process of both bacteria. The aim of this experiment is to identify the cross reaction that can be observed by checking the hemagglutination effect of adhesion mollecule 37,8 kDa from Vibrio cholerae after being given the antibody from adhesion mollecule 49,8 kDa of Shigella flexneri with true experimental design by conducting hemagglutination inhibition test. Positive result was found up to the titer of 1/64. Based on this experiment, it can be concluded that there is a cross reaction between adhesion mollecule 49,8 kDa of Shigella flexneri with adhesion mollecule 37,8 kDa Vibrio cholerae and it could be one of the consideration in the making of homologous vaccine.
Other obstract
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| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/536/051900998 |
| Uncontrolled Keywords: | Shigella flexneri, Vibrio cholerae, molekul adhesin, protein hemaglutinin, antihemaglutinasi, vaksin homolog, Shigella flexneri, Vibrio cholerae, adhesion molecule, hemagglutinin protein, hemagglutination inhibition, homologous vaccine |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.93 Clostridium infections, diphtheria, cholera, dysenteries, protozoan infections > 616.932 Cholera |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | Nur Cholis |
| Date Deposited: | 22 May 2020 11:06 |
| Last Modified: | 21 Oct 2021 07:36 |
| URI: | http://repository.ub.ac.id/id/eprint/167871 |
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