Fukata, Editya (2018) Identifikasi Berat Molekul Protein Biofilm Bakteri Vibrio cholerae. Sarjana thesis, Universitas Brawijaya.
Abstract
Vibrio cholerae merupakan bakteri patogen penyebab penyakit kolera. Peran biofilm dalam patogenesis diare berpotensi untuk dikembangkan menjadi vaksin kolera namun masih didapatkan keterbatasan penelitian tentang protein biofilm V. cholerae terutama di Indonesia. Penelitian ini bertujuan untuk mendapatkan isolat protein biofilm bakteri V. cholerae dan mengamati cirinya dari berat molekulnya. Penelitian ini menggunakan desain penelitian deskriptif eksploratif. Sampel V. cholerae yang digunakan berasal dari stock culture milik Lab. Mikrobiologi Fakultas Kedokteran Universitas Brawijaya. Vibrio cholerae ditanam pada medium trypticase soy broth dengan 1% glukosa. Untuk isolasi protein digunakan metode Chloroform Methanol Precipitation dan dilanjutkan separasi protein dengan SDS-PAGE. Diperoleh 2 band protein biofilm yang tidak ditemukan di sampel protein V. cholerae non-biofilm. Kemudian dihitung jarak migrasi dari 2 protein tersebut dan diukur berat molekulnya menggunakan regresi non-linear dari kurva standar dari protein marker GangNam-STAIN™. Hasil pengukuran menunjukkan 2 protein dengan berat molekul 80,9 kDa dan 14,6 kDa. Kedua protein ini diduga merupakan protein biofilm Bap1 dan Omp seperti yang ditemukan oleh penelitian terdahulu jika ditinjau dari kemiripan berat molekulnya. Bap1 dan Omp telah dikenal fungsinya dalam patogenesis kolera sehingga dua protein tersebut berpotensi untuk dikembangkan menjadi antigen protektif untuk vaksinasi kolera. Untuk membuktikannya perlu diteliti lebih lanjut tentang sifat adhesinya, misal dengan uji hemaglutinasi pada eritrosit tikus.
English Abstract
Vibrio cholerae is a pathogenic bacteria capable of causing cholera. The role of biofilm in pathogenesis of diarrhea indicate major potential to be developed as cholerae vaccine. However, the information regarding the characteristic of biofilm protein of V. cholerae is still relatively low particularly in Indonesia. This research was aimed to isolate the biofilm protein and identify its property based on the molecular weight. Descriptive and explorative design was used in this research. The sample used was obtained from the stock culture of Microbiology Laboratorium of Medical Faculty, Brawijaya University. Vibrio cholerae was cultured in trypticase soy broth medium with 1% glucose. In order to isolate the protein, we performed Chloroform Methanol Precipitation method and continued to protein separation using SDS-PAGE. 2 bands of biofilm protein were obtained. Then we measured the migration distance and calculated the molecular weight using non-linear regression of standard curve of GangNam-STAIN™ protein ladder. The result showed 2 protein with the molecular weight of 80,9 kDa dan 14,6 kDa. These proteins were assumed to be identical with two known biofilm proteins : Bap1 and Omp, considering their similar molecular weight. Bap1 and Omp have already established as their role in the pathogenesis of cholera thus their prospect to be developed as protective antigen for vaccine is high. However there is still further research needed to prove their adhesion ability, such as hemagglutination of mouse erythrocytes.
| Item Type: | Thesis (Sarjana) |
|---|---|
| Identification Number: | SKR/FK/2018/503/051900672 |
| Uncontrolled Keywords: | vibrio cholerae, protein biofilm, vaccine, Bap1, Omp-vibrio cholerae, biofilm protein, vaccine, Bap1, Omp |
| Subjects: | 600 Technology (Applied sciences) > 616 Diseases > 616.9 Other disease > 616.93 Clostridium infections, diphtheria, cholera, dysenteries, protozoan infections > 616.932 Cholera |
| Divisions: | Fakultas Kedokteran > Pendidikan Dokter |
| Depositing User: | soegeng sugeng |
| Date Deposited: | 12 May 2020 06:50 |
| Last Modified: | 12 May 2020 06:50 |
| URI: | http://repository.ub.ac.id/id/eprint/167743 |
Actions (login required)
 |
View Item |