Aktivitas Enzim L-Asparaginase Bakteri Paenibacillus sp. dan Bacillus subtilis RRM-1 Yang Dimutasi dengan Sinar Ultraviolet (UV)

Puspita, Fourina Indah (2018) Aktivitas Enzim L-Asparaginase Bakteri Paenibacillus sp. dan Bacillus subtilis RRM-1 Yang Dimutasi dengan Sinar Ultraviolet (UV). Sarjana thesis, Universitas Brawijaya.

Abstract

L-Asparaginase (E.C.3.5.1.1) merupakan enzim yang mengkatalis reaksi hidrolisis L-Asparagin menjadi asam aspartat dan amonia. Enzim L-Asparaginase dalam bidang kesehatan dapat dimaanfaatkan salah satunya digunakan sebagai agen antineoplastik dalam mengobati penyakit leukemia. L-Asparaginase juga dapat mencegah pembentukan akrilamid, senyawa karsinogenik pemicu kanker yang terdapat dalam produk abon ikan. L-Asparaginase dalam jumlah besar dapat dihasilkan oleh bakteri. Penelitian ini dilakukan pada bakteri endofit mangrove yaitu Paeniacillus sp. dan Bacillus subtilis RRM-1 yang memiliki aktivitas enzim L-Asparaginase tetapi dalam jumlah rendah pada penelitian sebelumnya. Teknik mutasi dapat dimanfaatkan untuk peningkatan produksi enzim pada mikroba yang lebih efisien. UV mutagenesis merupakan metode mutasi fisik yang paling sederhana dan efektif, menyebabkan perubahan struktur DNA, sehingga bakteri dapat menghasilkan enzim lebih maksimal. Pelaksanaan penelitian ini dilakukan di Laboratorium Keamanan Hasil Perikanan dan Laboratorium Parasit dan Penyakit Ikan Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Malang. Laboratorium Sentral Ilmu Hayati Universitas Brawijaya, Malang. Penelitian ini dilakukan mulai bulan Maret – September 2018. Tujuan dari dilakukannya penelitian ini adalah untuk mengetahui aktivitas enzim L-Asparaginase dari bakteri Paenibacillus sp. dan Bacillus subtilis RRM-1 yang dimutasi dengan sinar ultraviolet (UV). Metode yang digunakan pada penelitian ini adalah metode eksploratif deskriptif. Penelitian ini dilakukan dengan tiga tahap yaitu, tahap pertama peremajaan isolat bakteri Paeniacillus sp. dan Bacillus subtilis RRM-1. Tahap kedua melakukan proses mutagenesis bakteri menggunakan sinar ultraviolet (UV). Pada tahap ketiga melakukan uji aktivitas enzim L-Asparaginase dari bakteri mutan dengan survival rate 1% menggunakan media M9 broth modifikasi. Bakteri Paeniacillus sp. dan Bacillus subtilis RRM-1 yang dimutasi dengan sinar ultraviolet (UV) pada jarak 15 cm selama (0, 30, 60, 90 dan 120 menit) nilai survival rate terbaik adalah 1%. Survival rate terbaik yaitu waktu paparan sinar UV selama 120 menit. 8 bakteri mutan Paenibacillus sp. tidak menunjukkan adanya peningkatan aktivitas enzim L-Asparaginase, sedangkan dari 6 bakteri mutan Bacillus subtilis RRM-1 terdapat 2 mutan yang mengalami peningkatan aktivitas enzim L-Asparaginase yaitu mutan (UV-1) dan (UV-6). Saran untuk penelitian ini adalah sebaiknya dilakukan uji lanjut mengenai akitivitas enzim L-Asparaginase secara kuantitatif dan penelitian lanjutan mengenai stabilitas bakteri mutan penghasil enzim L-Asparaginase yang tinggi.

English Abstract

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Item Type: Thesis (Sarjana)
Identification Number: SKR/FPR/2018/908/051811907
Uncontrolled Keywords: -
Subjects: 500 Natural sciences and mathematics > 572 Biochemistry > 572.7 Enzymes
Divisions: Fakultas Perikanan dan Ilmu Kelautan > Manajemen Sumberdaya Perairan
Depositing User: soegeng sugeng
Date Deposited: 22 Oct 2019 02:19
Last Modified: 16 Mar 2022 01:46
URI: http://repository.ub.ac.id/id/eprint/165058
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