Nurlaili, Retti (2018) Peran Pemberian Kurkumin Terhadap Ekspresi Mmp-2 Dan Timp-2 Pada Hepatic Stellate Cells Tikus Model Fibrosis Hati Akibat Induksi Karbon Tetraklori. Magister thesis, Universitas Brawijaya.
Abstract
Latar Belakang: Fibrosis hati merupakan hasil proses fibrogenesis dan fibrolisis. Pada proses fibrogenesis, HSCs memproduksi MMP-2 dan TIMP-2. Keduanya berfungsi mengatur homeostasis matriks ekstraseluler. Kurkumin meningkatkan ekspresi MMP-2, menurunkan ekspresi TIMP-2 serta mendorong apoptosis HSCs, yang dengannya fibrogenesis menjadi terhambat. Peran kurkumin, ekspresi MMP-2 dan ekspresi TIMP-2 terhadap HSCs dalam proses fibrolisis belum banyak diteliti. Tujuan: Mengetahui hubungan pemberian kurkumin terhadap peningkatan ekspresi MMP-2 dan penurunan ekspresi TIMP-2 di HSCs tikus model fibrosis hati. Metode: Penelitian ini menerapkan metode eksperimental pada tikus jantan strain wistar. Terdapat 8 kelompok tikus yakni KN, KP2, KP5, KP9, dan KK2, KK5, KK9. Masing-masing terdiri dari 4 tikus sampel. KP dipapar CCl4 1cc/kgbb 2x perminggu intraperitoneal selama 9 minggu membentuk fibrosis F3. KN diinjeksi Normal Salin. Setelah diinjeksi CCl4, Kelompok Kontrol (KK2,KK5,KK9) diberikan plasebo dan Kelompok Perlakuan (KP2,KP5,KP9) diberikan kurkumin. Analisis ekspresi MMP-2 dan ekspresi TIMP-2 menggunakan uji t independen, ANOVA dan korelasi. Hasil: Terdapat peningkatan ekspresi MMP-2 pada KK9 dan penurunan ekspresi TIMP- 2 pada KK5 dan KK9; peningkatan ekspresi MMP-2 dan penurunan ekspresi TIMP-2 pada KP2, KP5, dan KP9. Kesimpulan: Peningkatan signifikan ekspresi MMP-2 dan penurunan signifikan ekspresi TIMP-2 pada tikus yang diberikan kurkumin selama 2 minggu, 5 minggu dan 9 minggu. Lama pemberian kurkumin berkorelasi dengan peningkatan ekspresi MMP-2 dan penurunan ekspresi TIMP-2 pada tikus model fibrosis hati
English Abstract
Background: Liver fibrosis result of fibrogenesis and fibrolysis. In fibrogenesis, MMP-2 and TIMP-2 produced by HSCs, regulate ECM homeostastic. Curcumin inhibit MMP-2, TIMP-2 expression and enhance HSCs apoptosis, which is inhibit fibrogenesis. Role of curcumin, MMP- 2 expression, and TIMP-2 expression in a fibrolysis has not been widely studied. Objective: Determine correlation between giving of curcumin to increase MMP-2 expression and decreased TIMP-2 expression rat model of liver fibrosis. Methods: Experimental study in male rats wistar strain. There are 8 groups KN, KP2, KP5, KP9, dan KK2, KK5, KK9; 4 rats for each. KP group was exposed to CCl4 1cc/kg intraperitoneally 2 times per week for 9 weeks to form F3 fibrosis. KN group was injected Normal saline. After CCl4 injected, Control group (KK) was given placebo and intervention group (KP) was given curcumin. Analysis of MMP-2 and TIMP-2 expression at HSCs is conducted by independent t-test, ANOVA and correlation test. Result: Increase MMP-2 expression in KK9 and decrease TIMP-2 expression in KK5 and KK9. Increase MMP-2 expression and decrease TIMP-2 expression in KP2, KP5, and KP9. Conclusion: Remarkable increase MMP-2 and remarkable decrease TIMP-2 expression in HSCs after giving of curcumin 2, 5 and 9 weeks. Duration curcumin correlated with increase MMP-2 expression and decrease TIMP-2 expression in rat model liver fibrosis
| Item Type: | Thesis (Magister) |
|---|---|
| Identification Number: | TES/615.324 39/NUR/p/2018/041804973 |
| Uncontrolled Keywords: | CURCUMA, LIVER, FIBROSIS, CARBON TETRACHLORIDE, METALLOPROTEINASES |
| Subjects: | 600 Technology (Applied sciences) > 615 Pharmacology and therapeutics > 615.3 Organics drugs > 615.32 Drugs derived from plants and mikroorganisms > 615.324 39 Drugs derived from specific plants (Ginger, Musaceae, Zingiberales) |
| Divisions: | Profesi Kedokteran > Spesialis Ilmu Penyakit Dalam, Fakultas Kedokteran |
| Depositing User: | Nur Cholis |
| Date Deposited: | 17 Dec 2018 07:04 |
| Last Modified: | 22 Oct 2021 03:55 |
| URI: | http://repository.ub.ac.id/id/eprint/162195 |
Preview |
Text
dr. Retti Nurlaili.pdf Download (5MB) | Preview |
Actions (login required)
 |
View Item |